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Y-27632 dihydrochloride

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Based on 607 publication(s) in Google Scholar

Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM). Y-27632 dihydrochloride shows antiepileptic effects.

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  • Pureté : 99.94%
  • CAS No.: 129830-38-2
  • Formule: C14H23Cl2N3O
  • Masse moléculaire:320.26
  • Stockage:

    4°C, sealed storage, away from moisture

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

  • Y-27632 dihydrochloride Chemical Structure
    Y-27632 dihydrochloride Chemical Structure
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
  • Experimental Validation Results for Y-27632 dihydrochloride
CAS No.: 129830-38-2
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  • Y-27632 dihydrochloride Chemical Structure
    Y-27632 dihydrochloride Chemical Structure
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    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Science. 2025 Jun 5;388(6751):eadu9375. [Abstract]

    Customer Validation
     On day 0, hPSCs were seeded onto new Matrigel- coated 6-well plates in StemMACS iPS-Brew XF medium + 10 μM Y-27632. On days 1 to 4, NC induction medium was added and changed daily. On days 5 and 6, NC induction medium + 1 μM retinoic acid was added daily. On day 7, cells were dissociated and split 1:6 in NC maintenance medium + 1 μM retinoic acid + 10 μM Y-27632. On days 8 and 9, NC maintenance medium + 1 μM retinoic acid was refreshed daily. On day 10, cells were dissociated and split. From days 11 to 21, SMC induction medium was refreshed daily. On days 22 to 28, cells were maintained in Medium 231+ Smooth Muscle Growth Supplement. Confocal fluorescence imaging and immunostaining confirms the differentiation of hESC-3R into CMs (coexpression of troponin-T with TNNT2-GFP), ECs (coexpression of VE-cadherin with CDH5-mOrange), and SMCs (coexpression of SM22α with TAGLN-CFP).

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    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Jun 10;42(6):1086-1105.e13. [Abstract]

    Customer Validation
     Y-27632 (GBCs; 10 uM). Representative H&E of primary RPM tumor (top) vs GBC-derived RPM allograft (bottom). Scale bars: 50 μm.

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell. 2024 Nov 14;187(23):6566-6583.e22. [Abstract]

    Customer Validation
     Naive hPSCs were seeded into 300 or 400-micrometer hydrogel microwells in N2B27 with Y-27632 (10 μM). The next day, the medium was changed to PALLY medium, and half of the medium was replaced with fresh PALLY daily. IF staining for the EPI (SOX2), HYPO (GATA4), and TE markers (GATA3) on control blastoids (day 4). TE, trophectoderm; EPI, epiblast; HYPO, hypoblast.

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Nature. 2022 Jan;601(7894):600-605. [Abstract]

    Customer Validation
     Upon exposure to lysophosphatidic acid (LPA) (a Hippo pathway inhibitor), A83-01 (an inhibitor of TGFβ family receptors) and PD0325901 (an ERK inhibitor) in a chemically defined medium containing the STAT activator leukaemia inhibitory factor (LIF) and Y-27632 (a ROCK inhibitor) (10 µM, 7 days),blastocyst-like structures formed efficiently from naive PSCs.

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Nature. 2022 Jan;601(7894):600-605. [Abstract]

    Customer Validation
     Upon exposure to lysophosphatidic acid (LPA) (a Hippo pathway inhibitor), A83-01 (an inhibitor of TGFβ family receptors) and PD0325901 (an ERK inhibitor) in a chemically defined medium containing the STAT activator leukaemia inhibitory factor (LIF) and Y-27632 (a ROCK inhibitor) (10 µM, 7 days), blastocyst-like structures formed efficiently from naive PSCs. trophectoderm (TE) cell analogues (identified as GATA2+GATA3+CDX2+TROP2+) formed, proliferated, and established adherens junctions (marked by CDH1), apical-basal polarity (indicated by aPKC) and tight junctions (marked by ZO-1).

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Oct 6;29(10):1475-1490.e6. [Abstract]

    Customer Validation
     The iPSCs were differentiated into lung airway organoids under s series of reagents which contains 10 μM Y-27632. The differentiation was confirmed by immunostaining with antibodies directed against airway markers, including E-Cadherin, FOXJ1 and acetylated α-tubulin, and MUC5AC

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2021 Sep 17;7(38):eabb5933. [Abstract]

    Customer Validation
     Y27632 dihydrochloride (5 mg/kg/day; i.p.) increases both IFN-I and IFN-III production in VSV-infected intestine organoids.

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]

    Customer Validation
     Fluorescence imaging of LNCaP cells treated with the F-actin inhibitor cytoskeleton B (CB), microtubule inhibitor nocodazole (NO), FAK inhibitor PF573288 (PF), ROCK inhibitor Y-27632 (Y), and myosin II (Myo-II) inhibitor (-)-blebbistatin ((-)Bl). After the cells were cultured on different substrates for 3 days, inhibitors were added and incubated for 24 h. (green, F-actin; scale bar, 25 µm).

  • 1/16

    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]

    Customer Validation
     Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).

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    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2018 Jun 28;37(1):128. [Abstract]

    Customer Validation
     MHCC-97H and Huh-7 cells co-transfected with control or supervillin-specific siRNA and a RhoA(WT), RhoA(V14), or RhoA(N19) plasmids for 48 h are treated with PBS or Y27632 2HCl (10 μM) for 16 h during hypoxia, and then assayed for phosphorylated p38 and ERK by immunoblotting. β-actin is used as the loading control

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    Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jul 31;8(47):82506-82530. [Abstract]

    Customer Validation
     SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) cells were either untreated (-) or treated (+) with 10mM Y27632 over a period of 48 hr. SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) clones were either untreated (-) or treated (+) with 10 mM Y27632 followed by stimulation without (-) or with (+) 5 ng/mL of TGFβ1 for 48 hr.

  • Y-27632 dihydrochloride