Ac-DMLD-CMK TFA
Based on 1 Customer Validation
Ac-DMLD-CMK TFA is a caspase 3 inhibitor and a GSDME inhibitor. Ac-DMLD-CMK TFA binds directly to the catalytic domain of caspase-3, blocks caspase-3-mediated cleavage of GSDME, inhibits the activation of caspase 3 and Gsdme in the caspase 3-Gsdme signaling pathway, and reduces the levels of pyroptosis and apoptosis as well as the expression of LDH, IL-6, IL-1β and IL-18. Ac-DMLD-CMK TFA alleviates renal function deterioration, renal tubular epithelial cell injury, inflammatory cytokine secretion, pulmonary structural damage, and chemotherapy-induced nephrotoxicity.
For research use only. We do not sell to patients.
- Purity: 98.44%
- Formula: C24H36ClF3N4O11S
- Molecular Weight:681.08
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Anti-Cancer Compound Library
HY-L025
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Pyroptosis Compound Library
HY-L059
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Neurodegenerative Disease-related Compound Library
HY-L086
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Peptide Library
HY-L105
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Antidepressant Compound Library
HY-L108
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Anti-Prostate Cancer Compound Library
HY-L124
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Pain-Related Compound Library
HY-L139
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Protease Inhibitor Library
HY-L147
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Peptide Compound Library
HY-L105S
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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High-Throughput Bioactive Compound Library
HY-L205
All Caspase Isoforms
More
Biological Activity
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Caspase 3 |
Ac-DMLD-CMK TFA (5 μM; 6 h pre-incubation) inhibits simvastatin-induced pyroptosis and cell viability reduction in MGC-803 human gastric cancer cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Ac-DMLD-CMK TFA (5 mg/kg; i.p.; single dose 3 h pre-CLP) significantly reduces CLP-induced acute lung injury in male C57BL/6 J mice by inhibiting caspase-3/GSDME-mediated pyroptosis and apoptosis, as evidenced by reduced lung injury scores, inflammatory mediator levels, and cell death markers[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (male, 6-8 weeks old, 20-25 g, cisplatin-induced)[1]
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Dosage:5 mg/kg/day
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Administration:i.p.; single injection 1 hour prior to cisplatin administration
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Result:Reduced serum creatinine and blood urea nitrogen levels compared to cisplatin-only treated mice.
Alleviated renal tubular epithelial cell death.
Reduced levels of cleaved mouse Gsdme (Gsdme-N) and cleaved caspase 3, with no significant effect on full-length mouse Gsdme (Gsdme-FL).
Suppressed renal mRNA expression of Ngal, Il6,Tnfa, and Il1b; did not affect Kim1 expression.
Chemical Information
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Appearance Solid
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Molecular Weight 681.08
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Formula C24H36ClF3N4O11S
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Color White to off-white
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Sequence
Ac-Asp-Met-Leu-{Asp-CMK}
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Sequence Shortening
Ac-DML-{Asp-CMK}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (146.83 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Shen X, et al. Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity. Cell Death Dis. 2021;12(2):186. Published 2021 Feb 15. [Content Brief]
[2]. Qin H, et al. Inhibiting caspase-3/GSDME-mediated pyroptosis ameliorates septic lung injury in mice model. Mol Immunol. 2024;172:96-104. [Content Brief]
[3]. Xia Y, et al. Antitumor Effect of Simvastatin in Combination With DNA Methyltransferase Inhibitor on Gastric Cancer via GSDME-Mediated Pyroptosis. Front Pharmacol. 2022;13:860546. Published 2022 Apr 20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4683 mL | 7.3413 mL | 14.6826 mL | 36.7064 mL |
| 5 mM | 0.2937 mL | 1.4683 mL | 2.9365 mL | 7.3413 mL | |
| 10 mM | 0.1468 mL | 0.7341 mL | 1.4683 mL | 3.6706 mL | |
| 15 mM | 0.0979 mL | 0.4894 mL | 0.9788 mL | 2.4471 mL | |
| 20 mM | 0.0734 mL | 0.3671 mL | 0.7341 mL | 1.8353 mL | |
| 25 mM | 0.0587 mL | 0.2937 mL | 0.5873 mL | 1.4683 mL | |
| 30 mM | 0.0489 mL | 0.2447 mL | 0.4894 mL | 1.2235 mL | |
| 40 mM | 0.0367 mL | 0.1835 mL | 0.3671 mL | 0.9177 mL | |
| 50 mM | 0.0294 mL | 0.1468 mL | 0.2937 mL | 0.7341 mL | |
| 60 mM | 0.0245 mL | 0.1224 mL | 0.2447 mL | 0.6118 mL | |
| 80 mM | 0.0184 mL | 0.0918 mL | 0.1835 mL | 0.4588 mL | |
| 100 mM | 0.0147 mL | 0.0734 mL | 0.1468 mL | 0.3671 mL |
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