Ac-FR-AFC TFA
Based on 1 Customer Validation
Ac-FR-AFC TFA is a fluorometric substrate for cathepsin L. Ac-FR-AFC TFA can be used for the research of glioblastoma.
For research use only. We do not sell to patients.
- Purity: 99.51%
- Formula: C29H30F6N6O7
- Molecular Weight:688.57
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Cathepsin Isoforms
More
Biological Activity
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cathepsin L |
Ac-FR-AFC TFA (incubated for 1.5 h) serves as an effective fluorescent substrate for detecting cathepsin L activity in cell lysates of human glioblastoma U251 and U87 cells[1].
Cells are lysed after treatment with PBS (control group) or 5 μM TFP for 24 h. The supernatant is collected, mixed with the cathepsin L substrate (AC-FR-AFC), and incubated at 37°C for 1.5 h. Samples are then detected using a fluorescence microplate reader, with the excitation/emission wavelengths set at 400 nm/505 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 688.57
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Formula C29H30F6N6O7
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Color White to light yellow
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Sequence
Ac-Phe-Arg-AFC
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Sequence Shortening
Ac-FR-AFC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (145.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4523 mL | 7.2614 mL | 14.5229 mL | 36.3071 mL |
| 5 mM | 0.2905 mL | 1.4523 mL | 2.9046 mL | 7.2614 mL | |
| 10 mM | 0.1452 mL | 0.7261 mL | 1.4523 mL | 3.6307 mL | |
| 15 mM | 0.0968 mL | 0.4841 mL | 0.9682 mL | 2.4205 mL | |
| 20 mM | 0.0726 mL | 0.3631 mL | 0.7261 mL | 1.8154 mL | |
| 25 mM | 0.0581 mL | 0.2905 mL | 0.5809 mL | 1.4523 mL | |
| 30 mM | 0.0484 mL | 0.2420 mL | 0.4841 mL | 1.2102 mL | |
| 40 mM | 0.0363 mL | 0.1815 mL | 0.3631 mL | 0.9077 mL | |
| 50 mM | 0.0290 mL | 0.1452 mL | 0.2905 mL | 0.7261 mL | |
| 60 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6051 mL | |
| 80 mM | 0.0182 mL | 0.0908 mL | 0.1815 mL | 0.4538 mL | |
| 100 mM | 0.0145 mL | 0.0726 mL | 0.1452 mL | 0.3631 mL |
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