2. Metabolic Enzyme/Protease Others
  3. Cathepsin Fluorescent Dye
  4. Ac-FR-AFC TFA

Ac-FR-AFC TFA is a fluorometric substrate for cathepsin L. Ac-FR-AFC TFA can be used for the research of glioblastoma.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-FR-AFC TFA

Ac-FR-AFC TFA Chemical Structure

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Based on 1 publication(s) in Google Scholar

Ac-FR-AFC TFA Related Antibodies

Top Publications Citing Use of Products

View All Cathepsin Isoform Specific Products:

View All Isoforms
Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ac-FR-AFC TFA is a fluorometric substrate for cathepsin L. Ac-FR-AFC TFA can be used for the research of glioblastoma[1].

IC50 & Target

cathepsin L

 

In Vitro

Ac-FR-AFC TFA (incubated for 1.5 h) serves as an effective fluorescent substrate for detecting cathepsin L activity in cell lysates of human glioblastoma U251 and U87 cells[1].
Cells are lysed after treatment with PBS (control group) or 5 μM TFP for 24 h. The supernatant is collected, mixed with the cathepsin L substrate (AC-FR-AFC), and incubated at 37°C for 1.5 h. Samples are then detected using a fluorescence microplate reader, with the excitation/emission wavelengths set at 400 nm/505 nm[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-FR-AFC TFA Related Antibodies
Molecular Weight

688.57

Formula

C29H30F6N6O7
Appearance

Solid

Color

White to light yellow

Sequence

Ac-Phe-Arg-AFC
Sequence Shortening

Ac-FR-AFC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (145.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4523 mL 7.2614 mL 14.5229 mL
5 mM 0.2905 mL 1.4523 mL 2.9046 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4523 mL 7.2614 mL 14.5229 mL 36.3071 mL
5 mM 0.2905 mL 1.4523 mL 2.9046 mL 7.2614 mL
10 mM 0.1452 mL 0.7261 mL 1.4523 mL 3.6307 mL
15 mM 0.0968 mL 0.4841 mL 0.9682 mL 2.4205 mL
20 mM 0.0726 mL 0.3631 mL 0.7261 mL 1.8154 mL
25 mM 0.0581 mL 0.2905 mL 0.5809 mL 1.4523 mL
30 mM 0.0484 mL 0.2420 mL 0.4841 mL 1.2102 mL
40 mM 0.0363 mL 0.1815 mL 0.3631 mL 0.9077 mL
50 mM 0.0290 mL 0.1452 mL 0.2905 mL 0.7261 mL
60 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6051 mL
80 mM 0.0182 mL 0.0908 mL 0.1815 mL 0.4538 mL
100 mM 0.0145 mL 0.0726 mL 0.1452 mL 0.3631 mL
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Ac-FR-AFC TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-FR-AFCCathepsinFluorescent Dyehuman glioblastoma U87 cell lysateshuman glioblastoma U251 cell lysatesglioblastomacathepsin LInhibitorinhibitorinhibit

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