2. Cell Cycle/DNA Damage
    Epigenetics
    Vitamin D Related/Nuclear Receptor
    Metabolic Enzyme/Protease
    Apoptosis
    Anti-infection
  3. Sirtuin
    PPAR
    Fatty Acid Synthase (FASN)
    c-Myc
    Bacterial
  4. Agrimol B

Agrimol B 

Cat. No.: HY-N0704 Purity: 99.72%
COA Handling Instructions

Agrimol B, a polyphenol, is an orally active and potent SIRT1 activator. Agrimol B shows anti-adipogenic and anticancer activity. Agrimol B shows antibacterial activity against plant pathogens. Agrimol B dramatically inhibits 3T3-L1 adipocyte differentiation by reducing PPARγ, C/EBPα, FAS, UCP-1, and apoE expression. The action of Agrimol B on the cancer cells is likely derived from its effect on c-MYC, SKP2 and p27.

For research use only. We do not sell to patients.

Agrimol B Chemical Structure

Agrimol B Chemical Structure

CAS No. : 55576-66-4

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5 mg USD 403 In-stock
Estimated Time of Arrival: December 31
10 mg USD 686 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Agrimol B, a polyphenol, is an orally active and potent SIRT1 activator. Agrimol B shows anti-adipogenic and anticancer activity. Agrimol B shows antibacterial activity against plant pathogens. Agrimol B dramatically inhibits 3T3-L1 adipocyte differentiation by reducing PPARγ, C/EBPα, FAS, UCP-1, and apoE expression. The action of Agrimol B on the cancer cells is likely derived from its effect on c-MYC, SKP2 and p27[1][2][3].

IC50 & Target[1]

SIRT1

 

PPARγ

 

In Vitro

Agrimol B blocks adipogenesis at the early stage of differentiation in a dose-dependent manner, with an IC50 of 3.35 ± 0.32 μM[1].
Agrimol B induces SIRT1 (silent information regulator 2 homolog 1) translocation and expression in 3T3-L1 adipocytes[1].
Agrimol B inhibits PC-3 and A549 cells growth, with GI50 (growth inhibition 50%) values of 29 and 19 μM, GI75 values of 49 and 50 μM, and GI90 values of 63 and 76 μM, respectively[2].
Agrimol B (0-76 μM) dose-dependently increases cells at G0 in both cell lines[2].
Agrimol B (0-76 μM, 3 days) reduces the protein expression of c-MYC and SKP2 (S-phase kinase-associated protein 2), increases p27 (cyclin-dependent kinase inhibitor 1B), and down-regulates SPT16 (Suppressor of Ty Homolog-16) and SSRP1 (Structure-Specific Recognition Protein 1)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis[2]

Cell Line: PC-3 and A549 cells
Concentration: 29 and 63 μM (PC-3), 19 and 76 μM (A549)
Incubation Time: 10 min
Result: Dose-dependently increased cells at G0 in both cell lines. Reduced percentage of cells positive for Ki-67, and increased p27 positive cell population in PC-3 and A549 cells.

Western Blot Analysis[2]

Cell Line: PC-3 and A549 cells
Concentration: 0, 29, 49, 63 μM (PC-3); 0, 19, 50, and 76 μM (A549)
Incubation Time: 3 days
Result: Reduced c-MYC, SKP2 and increased p27 in both cell lines, and down-regulated SPT16 and SSRP1 in A549 cells with no effect on CRM1 in both cell lines.

Immunofluorescence[1]

Cell Line: 3T3-L1 preadipocyte
Concentration: 0, 3, and 10 μM
Incubation Time: 6 days
Result: Significantly increased nuclear positive rate of SIRT1; Markedly increased SIRT1 expression at 10 μM, the effect vanished at 3 μM. Down-regulated PPARγ and C/EBPα (CCAAT/enhancer-binding protein α) expression; Significantly decreased FAS (fatty acid synthesis), UCP-1 (uncoupling protein-1), and apoE (apolipoprotein E) expression at 10 μM.
In Vivo

Agrimol B (10 mg/kg, Orally, daily) reduces growth of prostate cancer cell xenograft in mice[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice (6-week-old, male, injected with PC-3 cells in 0.2 mL PBS subcutaneously)[2]
Dosage: 10 mg/kg
Administration: Orally, daily, after 15 days of cancer cell implantation
Result: Inhibited tumor growth in a mouse model of human prostate cancer, reduced the tumor volume at day 31 and day 32.
Molecular Weight

682.75

Appearance

Solid

Formula

C37H46O12
CAS No.
SMILES

CC[[email protected]](C)C(C1=C(O)C(CC2=C(O)C(C(CCC)=O)=C(OC)C(C)=C2O)=C(O)C(CC3=C(O)C(C(CCC)=O)=C(OC)C(C)=C3O)=C1O)=O
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (12.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4647 mL 7.3233 mL 14.6467 mL
5 mM 0.2929 mL 1.4647 mL 2.9293 mL
10 mM 0.1465 mL 0.7323 mL 1.4647 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.43 mg/mL (2.09 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.75%

COA (254 KB) HNMR (303 KB) RP-HPLC (255 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Agrimol B55576-66-4SirtuinPPARFatty Acid Synthase (FASN)c-MycBacterialPeroxisome proliferator-activated receptorsMycAgrimonia pilosa Ledeb.Lipid dropletObesityPPARγSIRT1Anti-adipogenicAntibacterialQuiescent cancer cellsProstate cancerLung cancerPolyphenolsadipogenesisInhibitorinhibitorinhibit

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