Amorfrutin B
Amorfrutin B is a highly potent natural peroxisome proliferation-activated receptor γ (PPARγ) agonist with oral activity with Ki values of 19 nM and EC50 values of 73 nM, respectively. Amorfrutin B has hypoglycemic and neuroprotective activities.
For research use only. We do not sell to patients.
- CAS No.: 78916-42-4
- Formula: C26H32O4
- Molecular Weight:408.53
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
0.05 μM
Compound: amorfrutin B
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Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay
Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay
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[PMID: 23286787] |
| HEK293 | EC50 |
73 nM
Compound: 2
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Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay
Agonist at human PPARgamma LBD expressed in human HEK293 cells cotransfected with GAL4-Luc assessed as transcriptional activation by luciferase reporter gene assay
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[PMID: 25938459] |
| HT-29 | IC50 |
20 μM
Compound: 1
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Antiproliferative activity against human HT-29 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay
Antiproliferative activity against human HT-29 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay
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[PMID: 26731300] |
| HT-29 | IC50 |
30.5 μM
Compound: 2
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Reduction in TNFalpha-induced PPARgamma expression in human HT-29 cells after 48 hrs by Western blotting analysis
Reduction in TNFalpha-induced PPARgamma expression in human HT-29 cells after 48 hrs by Western blotting analysis
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[PMID: 25938459] |
| MCF7 | IC50 |
33 μM
Compound: 1
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Antiproliferative activity against human MCF7 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay
Antiproliferative activity against human MCF7 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay
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[PMID: 26731300] |
| PC-3 | IC50 |
32 μM
Compound: 1
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Antiproliferative activity against human PC3 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay
Antiproliferative activity against human PC3 cells assessed as cellular DNA content after 72 hrs by CyQUANT NF fluorescence assay
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[PMID: 26731300] |
| T84 | IC50 |
13 μM
Compound: 1
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Antiproliferative activity against human T84 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay
Antiproliferative activity against human T84 cells assessed as cellular DNA content after 96 hrs by CyQUANT NF fluorescence assay
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[PMID: 26731300] |
In Vitro
Amorfrutin B (1,5 μM, 6 h) inhibits apoptosis and autophagy by gene methylation and miRNA-dependent regulation, and protects mouse brain neurons from hypoxia/ischemia[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:High-fat diet mice model[2].
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Dosage:100 mg/kg
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Administration:Add to food，2 weeks
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Result:Reduced fasting plasma levels of triacylglycerols by 25% and the concentrations of deleterious plasma NEFA by 29%.
Chemical Information
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CAS No. 78916-42-4
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Appearance Solid
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Molecular Weight 408.53
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Formula C26H32O4
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Color White to yellow
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SMILES
O=C(O)C1=C(CCC2=CC=CC=C2)C=C(OC)C(C/C=C(C)/CC/C=C(C)\C)=C1O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Przepiórska K, et al. Amorfrutin B Protects Mouse Brain Neurons from Hypoxia/Ischemia by Inhibiting Apoptosis and Autophagy Processes Through Gene Methylation- and miRNA-Dependent Regulation. Mol Neurobiol. 2023 Feb;60(2):576-595. [Content Brief]
[2]. Weidner C, et al. Amorfrutin B is an efficient natural peroxisome proliferator-activated receptor gamma (PPARγ) agonist with potent glucose-lowering properties. Diabetologia. 2013 Aug;56(8):1802-12. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Amorfrutin B Related Classifications
HY-113631 Related Classifications
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