Anti-Human/Mouse GRP78 Antibody (N88)
Anti-Human/Mouse GRP78 Antibody (N88) is an anti-human/mouse GRP78 IgG1 monoclonal antibody. Anti-Human/Mouse GRP78 Antibody (N88) can activate the Akt signaling pathway. Anti-Human/Mouse GRP78 Antibody (N88) can inhibit the expression of inflammatory factors and plasminogen activator inhibitors induced by TLR agonists. Anti-Human/Mouse GRP78 Antibody (N88) can be used for research on inflammation conditions. Anti-Human/Mouse GRP78 Antibody (N88) can be used for immunoprecipitation.
For research use only. We do not sell to patients.
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human/Mouse
IC50 & Target
[1]|
IL-6 |
IL-1β |
In Vitro
Anti-Human/Mouse GRP78 Antibody (N88) (1 μg/mL, 1 h or 6 h) significantly inhibits the expression of TNF α, IL-6, IL-1β, CCL2, CCL3, and CCL4 induced by LPS (HY-D1056) and reduces the phosphorylation of IκBα in bone marrow-derived macrophages (BMDMs)[1].
Anti-Human/Mouse GRP78 Antibody (N88) (1 μg/mL, 1 h or 6 h) widely inhibits the inflammatory response and inhibits the expression of PAI1 and PAI2 induced by multiple TLR agonists (Lipoteichoic acid (LTA) (HY-N9481), Imiquimod (IMQ) (HY-B0180), or ODN 1826 (HY-146245)) in BMDMs[1].
Anti-Human/Mouse GRP78 Antibody (N88) (1 μg/mL, 15 min) activates Akt phosphorylation in a dose-dependent manner in B16F1 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BMDMs
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Concentration:1 μg/mL, or co-incubation with LTA (1 μg/mL), IMQ (3 μg/mL) or ODN 1826 (1 μg/mL)
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Incubation Time:6 h
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Result:Significantly reduced the mRNA level of TNF α, IL-6, IL-1β, CCL2, CCL3, and CCL4.
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Cell Line:BMDMs
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Concentration:1 μg/mL, or co-incubation with LTA (1 μg/mL), IMQ (3 μg/mL) or ODN 1826 (1 μg/mL)
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Incubation Time:1 h
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Result:Reduced the phosphorylation of IκBα.
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Cell Line:BMDMs
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Concentration:1 μg/mL, or co-incubation with LTA (1 μg/mL), IMQ (3 μg/mL) or ODN 1826 (1 μg/mL)
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Incubation Time:1 h
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Result:Reduced the mRNA level of PAI1 and PAI2.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5000 B16F1 cells injected female C57BL/6 mice (4-6 weeks)[3]
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Dosage:200 μg
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Administration:Intraperitoneal injection (i.p.), once every 14 days
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Result:Significantly accelerated tumor growth.
Shortened median survival to 19 days.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Product Image
Application
in vivo administration; Functional assays
Chemical Information
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Molecular Weight 150 kDa
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SMILES
[Anti-Human/Mouse GRP78 Antibody (N88)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Gunner CB, et al. An antibody that targets cell-surface glucose-regulated protein-78 inhibits expression of inflammatory cytokines and plasminogen activator inhibitors by macrophages. J Cell Biochem. 2023 May;124(5):743-752. [Content Brief]
[2]. Trink J, et al. Integrin β1/Cell Surface GRP78 Complex Regulates TGFβ1 and Its Profibrotic Effects in Response to High Glucose. Biomedicines. 2022 Sep 10;10(9):2247. [Content Brief]
[3]. de Ridder GG, et al. A murine monoclonal antibody directed against the carboxyl-terminal domain of GRP78 suppresses melanoma growth in mice. Melanoma Res. 2012 Jun;22(3):225-35. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Human/Mouse GRP78 Antibody (N88) Related Classifications
HY-P990239 Related Classifications
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