Anti-inflammatory agent 112 

Anti-inflammatory agent 112 (compound 3f) is an inducible nitric oxide synthase (iNOS) inhibitor, a cyclooxygenase-2 (COX-2) inhibitor, and an anti-inflammatory agent. Anti-inflammatory agent 112 suppresses i-NOS and COX-2 protein expression, reduces nitric oxide, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) production, and attenuates lipopolysaccharide (LPS)-induced inflammatory responses. Anti-inflammatory agent 112 can be used for the research of inflammatory disorders^[1].

Anti-inflammatory agent 112 (compound 3f) (1-10 μM; 48 h) maintains over 90% cell viability in RAW264.7 macrophages^[1].
Anti-inflammatory agent 112 (compound 3f) (1-10 μM; 1 h pre-treatment before 24 h LPS stimulation) inhibits LPS-induced NO production in RAW264.7 macrophages with 54.3% inhibition at 10 μM, acting in a concentration-dependent manner^[1].
Anti-inflammatory agent 112 (1-10 μM; pre-treated before 24 h LPS induction) significantly reduces LPS-induced IL-6 and TNF-α release in RAW264.7 macrophages, with a concentration-dependent effect^[1].
Anti-inflammatory agent 112 (5-10 μM; 1 h pre-treatment before 24 h LPS stimulation) suppresses LPS-induced i-NOS and COX-2 protein expression in RAW264.7 macrophages^[1].
Anti-inflammatory agent 112 binds to 1DD7, 1NFK, 2Z64, and 5COX with binding energies ranging from -5.16 to -9.17 kcal/mol, showing favorable interactions with most targets compared to compound 3d^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

392.45

C₂₂H₂₄N₄O₃

O=C(OC)CCNC1=C(N(C)C)N=C(C2=CC=CC=C2)N(C3=CC=CC=C3)C1=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Naikoo RA, et al. Design, Synthesis, and Anti-Inflammatory Activity of Functionalized 1,2,4,5-Tetrasubstituted-Pyrimidinone. Chem Biodivers. 2026;23(1):e02861.  [Content Brief]