Art. -Nr.: HY-N0072

Brazilin

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

Brazilin is a red dye precursor obtained from the heartwood of several species of tropical hardwoods. Brazilin inhibits the cells proliferation, promotes apoptosis, and induces autophagy through the AMPK/mTOR pathway. Brazilin shows chondroprotective and anti-inflammatory activities.

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Reinheit: 99.17%
CAS. Nr.: 474-07-7
Formel: C₁₆H₁₄O₅
Molecular Weight:286.28

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Brazilin Chemical Structure

CAS. Nr.: 474-07-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	15.56 μg/mL Compound: 2, Brazilin	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 20036537]
A549	IC₅₀	54.41 μM Compound: 3, CS-3	Cytotoxicity against human A549 cells after 3 days by MTT assay Cytotoxicity against human A549 cells after 3 days by MTT assay	[PMID: 21800859]
Ca9-22	IC₅₀	34.2 μM Compound: 3, CS-3	Cytotoxicity against human Ca9-22 cells after 3 days by MTT assay Cytotoxicity against human Ca9-22 cells after 3 days by MTT assay	[PMID: 21800859]
Ca9-22	IC₅₀	9.78 μg/mL Compound: 2, Brazilin	Cytotoxicity against human Ca9-22 cells Cytotoxicity against human Ca9-22 cells	[PMID: 20036537]
Hep 3B2	IC₅₀	15.87 μM Compound: 3, CS-3	Cytotoxicity against human Hep3B cells after 3 days by MTT assay Cytotoxicity against human Hep3B cells after 3 days by MTT assay	[PMID: 21800859]
Hep 3B2	IC₅₀	4.54 μg/mL Compound: 2, Brazilin	Cytotoxicity against human Hep3B cells Cytotoxicity against human Hep3B cells	[PMID: 20036537]
HepG2	IC₅₀	12.03 μM Compound: 3, CS-3	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 21800859]
HepG2	IC₅₀	3.47 μg/mL Compound: 2, Brazilin	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 20036537]
MCF7	IC₅₀	21.99 μM Compound: 3, CS-3	Cytotoxicity against human MCF7 cells after 3 days by MTT assay Cytotoxicity against human MCF7 cells after 3 days by MTT assay	[PMID: 21800859]
MCF7	IC₅₀	6.29 μg/mL Compound: 2, Brazilin	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 20036537]
MCF7	IC₅₀	7.23 μM Compound: Brazilien	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 68 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 68 hrs by MTT assay	[PMID: 29306837]
MDA-MB-231	IC₅₀	10.8 μM Compound: 3, CS-3	Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay Cytotoxicity against human MDA-MB-231 cells after 3 days by MTT assay	[PMID: 21800859]
MDA-MB-231	IC₅₀	3.09 μg/mL Compound: 2, Brazilin	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 20036537]
Neutrophil	IC₅₀	16.7 μM Compound: 2, Brazilin	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced elastase release Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced elastase release	[PMID: 20036537]
Neutrophil	IC₅₀	4.6 μM Compound: 2, Brazilin	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced superoxide anion generation Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/cytochalasin B-induced superoxide anion generation	[PMID: 20036537]
RAW264.7	IC₅₀	43.5 μM Compound: 5	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 23127886]

In Vitro

Brazilin (40 µM, 24 h) shows no significant cytotoxic effect on PC12 cells^[4].
Brazilin (10 μM, 1 h) reduces H2O2-induced (200 μM, 24 h) cytotoxicity in PC12 Cells^[4].
Brazilin (10 μM, 1 h) antagonists H2O2-induced (200 μM, 24 h) cytotoxicity in PC12 Cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

PC12 cell line
Concentration:

10, 20 μM
Incubation Time:

1 h
Result:

Attenuated 200 µM H2O2-induced decrease in cell viability at the concentration of 10 µM and 20 µM.(47.83 and 91.51% of the control value, respectively)

In Vivo

Brazilin (10 mg/kg, i.p, 28 d) alleviates the CMS-induced depressive- and anxiety-like behaviors in adult mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Chronic mild stress (CMS) mice model^[4]
Dosage:

10 mg/kg
Administration:

Intraperitoneally injected (i.p.), once a day for 28 d
Result:

Decreased the prolonged latency to feeding significantly.

Chemical Information

CAS. Nr. 474-07-7
Appearance Solid
Molecular Weight 286.28
Formel C₁₆H₁₄O₅
Color Yellow to brown
SMILES

OC1=CC=C([C@]2([H])[C@](CC3=C2C=C(O)C(O)=C3)(O)CO4)C4=C1
Structure Classification
- Phenols
- Polyphenols
- Others
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

Apoptosis

Brazilin alleviates acute lung injury via inhibition of ferroptosis through the SIRT3/GPX4 pathway. [Abstract]

2025 Apr;30(3-4):768-783. PMID: 39720978
Histol Histopathol

Brazilin attenuates kidney ischemia-reperfusion injury by regulating inflammation, oxidative stress, and mitochondrial dysfunction. [Abstract]

2025 Sep 9:18982. PMID: 40922656

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 33.33 mg/mL (116.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4931 mL

17.4654 mL

34.9308 mL

5 mM

0.6986 mL

3.4931 mL

6.9862 mL

10 mM

0.3493 mL

1.7465 mL

3.4931 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Dapson RW, et al. Brazilwood, sappanwood, brazilin and the red dye brazilein: from textile dyeing and folk medicine to biological staining and musical instruments. Biotech Histochem. 2015;90(6):401-23. [Content Brief]

[2]. Jia Y, et al. [Effect of brazilin on apoptosis and autophagy of tongue cancer Tca8113 cells and its molecular mechanism]. Nan Fang Yi Ke Da Xue Xue Bao. 2019 Mar 30;39(3):351-356. [Content Brief]

[3]. Weinmann D, et al, Brazilin blocks catabolic processes in human osteoarthritic chondrocytes via inhibition of NFKB1/p50. J Orthop Res. 2018 Sep;36(9):2431-2438. [Content Brief]

[4]. Xi Wang, et al. Brazilin Treatment Produces Antidepressant- and Anxiolytic-Like Effects in Mice. Biol Pharm Bull. 2019, 42, 8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4931 mL	17.4654 mL	34.9308 mL	87.3271 mL
	5 mM	0.6986 mL	3.4931 mL	6.9862 mL	17.4654 mL
	10 mM	0.3493 mL	1.7465 mL	3.4931 mL	8.7327 mL
	15 mM	0.2329 mL	1.1644 mL	2.3287 mL	5.8218 mL
	20 mM	0.1747 mL	0.8733 mL	1.7465 mL	4.3664 mL
	25 mM	0.1397 mL	0.6986 mL	1.3972 mL	3.4931 mL
	30 mM	0.1164 mL	0.5822 mL	1.1644 mL	2.9109 mL
	40 mM	0.0873 mL	0.4366 mL	0.8733 mL	2.1832 mL
	50 mM	0.0699 mL	0.3493 mL	0.6986 mL	1.7465 mL
	60 mM	0.0582 mL	0.2911 mL	0.5822 mL	1.4555 mL
	80 mM	0.0437 mL	0.2183 mL	0.4366 mL	1.0916 mL
	100 mM	0.0349 mL	0.1747 mL	0.3493 mL	0.8733 mL

Keywords

Brazilin

2 Cited Publications

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Brazilin

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Publications Citing Use of MedChemExpress (MCE) Brazilin

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Brazilin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Phenols Library

Traditional Chinese Medicine Active Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Cell Death Library

Bioactive Compound Library Max

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

3D Diverse Fragment Library

Publications Citing Use of MedChemExpress (MCE) Brazilin

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Brazilin Related Classifications

HY-N0072 Related Classifications