FIDAS-3
Based on 1 Customer Validation
FIDAS-3 is a stilbene derivative and is a potent Wnt inhibitor with an IC50 of 4.9 μM for methionine S-adenosyltransferase 2A (MAT2A). FIDAS-3 effectively competes against S-adenosylmethionine (SAM) for MAT2A binding. FIDAS-3 has anticancer activities.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.40%
- CAS. Nr.: 1266684-01-8
- Formel: C16H15F2N
- Molecular Weight:259.29
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Stem Cell Signaling Compound Library
HY-L017
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Wnt/Hedgehog/Notch Compound Library
HY-L020
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Differentiation Inducing Compound Library
HY-L038
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HY-L060
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Cancer Stem Cells Compound Library
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Biologische Aktivität
IC50: 4.9 μM (Methionine S-adenosyltransferase 2A (MAT2A))[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
32.1 μM
Compound: 1a
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Inhibition of human ERG over-expressed in HEK293 cell membrane by [3H]dofetilide binding assay
Inhibition of human ERG over-expressed in HEK293 cell membrane by [3H]dofetilide binding assay
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[PMID: 24950374] |
| LS174T | IC50 |
26.9 nM
Compound: 1a
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Antiproliferative activity against human LS 174T cells by cell viability analyzer
Antiproliferative activity against human LS 174T cells by cell viability analyzer
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[PMID: 24950374] |
| LS174T | IC50 |
59 nM
Compound: 1
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Antiproliferative activity against human LS 174T cells after 4 days by Vi-Cell cell viability analyzer
Antiproliferative activity against human LS 174T cells after 4 days by Vi-Cell cell viability analyzer
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[PMID: 24930834] |
FIDAS-3 (3 μM; 7 days; LS174T cells) treatment significantly inhibits the proliferation of LS174T cells[1].
FIDAS-3 (3-10 μM) treatment inhibits the expression of c-Myc and cyclinD1 in LS174T CRC cells. And FIDAS-3 induces the expression of cell cycle inhibitor, p21WAF1/CIP1[1].
FIDAS-3 (10 μM; 36 h) treatment reduces the levels of both S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in LS174T cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LS174T colorectal cancer (CRC) cells
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Concentration:3 μM
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Incubation Time:7 days
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Result:Significantly inhibited the proliferation of LS174T cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J athymic nude mice (6-8 week) injected with LS174 cells[2]
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Dosage:20 mg/kg
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Administration:Intraperitoneal injection; daily; for one months
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Result:Significantly inhibited the growth of xenograft tumors.
Chemical Information
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CAS. Nr. 1266684-01-8
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Appearance Solid
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Molecular Weight 259.29
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Formel C16H15F2N
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Color Off-white to light green
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SMILES
CN(C)C1=CC=C(/C=C/C2=C(F)C=CC=C2F)C=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (385.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Zhang W, et al. Fluorinated N,N-dialkylaminostilbenes repress colon cancer by targeting methionine S-adenosyltransferase 2A. ACS Chem Biol. 2013 Apr 19;8(4):796-803. [Content Brief]
[2]. Zhang W, et al. Fluorinated N,N-dialkylaminostilbenes for Wnt pathway inhibition and colon cancer repression. J Med Chem. 2011 Mar 10;54(5):1288-97. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8567 mL | 19.2834 mL | 38.5669 mL | 96.4171 mL |
| 5 mM | 0.7713 mL | 3.8567 mL | 7.7134 mL | 19.2834 mL | |
| 10 mM | 0.3857 mL | 1.9283 mL | 3.8567 mL | 9.6417 mL | |
| 15 mM | 0.2571 mL | 1.2856 mL | 2.5711 mL | 6.4278 mL | |
| 20 mM | 0.1928 mL | 0.9642 mL | 1.9283 mL | 4.8209 mL | |
| 25 mM | 0.1543 mL | 0.7713 mL | 1.5427 mL | 3.8567 mL | |
| 30 mM | 0.1286 mL | 0.6428 mL | 1.2856 mL | 3.2139 mL | |
| 40 mM | 0.0964 mL | 0.4821 mL | 0.9642 mL | 2.4104 mL | |
| 50 mM | 0.0771 mL | 0.3857 mL | 0.7713 mL | 1.9283 mL | |
| 60 mM | 0.0643 mL | 0.3214 mL | 0.6428 mL | 1.6070 mL | |
| 80 mM | 0.0482 mL | 0.2410 mL | 0.4821 mL | 1.2052 mL | |
| 100 mM | 0.0386 mL | 0.1928 mL | 0.3857 mL | 0.9642 mL |
FIDAS-3 Related Classifications
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