GI-Y2

GI-Y2 suppresses LPS+Nigericin (HY-127019)-induced pyroptosis in PMA-differentiated THP-1 cells with an IC₅₀ of 35.40 μM by binding to the Arg10 residue of GSDMD via hydrogen bonding to reduce GSDMD-NT membrane localization^[1][2].
GI-Y2 (2.5-40 μM) directly binds to purified GST-GSDMD protein with a K_d value of 36.0 μM^[1].
GI-Y2 (10 μM; 1 h) directly interacts with Flag-GSDMD expressed in HEK/293T cells, as evidenced by reduced pronase-mediated degradation of GSDMD at 10 μM^[1].
GI-Y2 inhibits GSDMD-mediated pyroptosis in human AC16, HEK-293T, and HepG2 cell lines^[1].
GI-Y2 (10 μM; 1 h) reduces GSDMD-N membrane binding and pyroptotic LDH release in HEK/293T cells expressing wild-type Flag-GSDMD, but not in cells expressing the GSDMD^R10A mutant, confirming Arg10 as the critical binding site^[1].
GI-Y2 (10-20 μM; 1 h) dose-dependently inhibits ox-LDL-induced pyroptosis in ApoE^−/− mouse primary peritoneal macrophages, reducing lipid uptake, inflammatory cytokine production, and GSDMD-N activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.