TPE-MI (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1 Stock solution^[1]
Dissolve TPE-MI in DMSO to make a 1 mM to 50 mM stock solution.
2 Preparation of dye working solution
1) Dilute the stock solution to a working concentration (typically 50 μM) in PBS before use.
Note: Please adjust the concentration of TPE-MI working solution according to the actual situation and prepare it before use.
3 Specific staining steps
1) Cell culture preparation: Seed cells (e.g. HeLa, Neuro2a cells) in a suitable culture container (e.g. 8-well μ-slides).
2) Washing: Wash cells with PBS and remove culture medium.
3) Staining: Add 50 μM TPE-MI working solution to the cells and incubate at 37 °C for 30 min.
4) Post-staining treatment:
Flow cytometry: Remove TPE-MI solution, digest the cells using appropriate methods (such as trypsin digestion, Accutase) , resuspend in PBS and centrifuge (120 g, 6 min) , discard the supernatant, and resuspend the cells in PBS for analysis.
SDS-PAGE detection: Lyse the stained cells with cell lysis buffer, determine the protein concentration, and perform fluorescence and Coomassie brilliant blue staining analysis after 12% polyacrylamide SDS-PAGE electrophoresis.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.