2. Neuronal Signaling Anti-infection
  3. Huntingtin Parasite
  4. TPE-MI

TPE-MI  (Synonyms: Tetraphenylethene maleimide)

Cat. No.: HY-143218 Purity: 98.93%
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TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells (the excitation wavelength is 350 nm and the emission wavelength is 470 nm). TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin research of the malaria parasites Plasmodium falciparum .

For research use only. We do not sell to patients.

CAS No. : 1245606-71-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
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500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of TPE-MI:

TPE-MI Related Antibodies

Top Publications Citing Use of Products

    TPE-MI purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Nov 10.  [Abstract]

    MFI of TPE‐MI in single CTCs and CTC clusters, as determined by flow cytometry (n = 5).

    TPE-MI purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821.  [Abstract]

    TPE-MI (50 μM, 30 min). Misfolded proteins in ERG11/erg11Δ mutants were analyzed after treatment with 16 μg/mL ellipticine and 4 μg/mL FLC.

    TPE-MI purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 May 25:S2090-1232(25)00370-4.  [Abstract]

    TPE-MI (50 µM, 2h). Mitochondrial unfolded protein loading was measured by TPE-MI fluorescence for 2 h, and the fluorescence intensity at 2 h was compared among the groups (three independent replicates).

    TPE-MI purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Dec 5;11(49):eaea7451.  [Abstract]

    Misfolded/unfolded protein contents in control and Rbfox2-deficient HSCs assessed with TPE-MI (150 μM, at 37°C for 45 min) dye. Representative histograms of TPE-MI intensity are shown in. Quantification is shown in.

    TPE-MI purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Sep 3;23(1):393.  [Abstract]

    Effect of MC on cellular protein homeostasis in MDA-MB-231 cells. Each bar indicates the mean ± SEM of fluorescence intensity of TPE-MI-stained (50 μM) cells from three replicates and 2 independent experiments were performed.

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    Description

    TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells (the excitation wavelength is 350 nm and the emission wavelength is 470 nm). TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin research of the malaria parasites Plasmodium falciparum [1][2].

    IC50 & Target

    Plasmodium

     

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Preparation of Dye Stock Solution[1]
    1.1 Dissolve TPE-MI in DMSO to prepare a stock solution with a concentration of 50 mM.
    Note: It is recommended to aliquot the TPE-MI stock solution and store it at -20℃ or -80℃ protected from light.
    2. Preparation of Dye Working Solution
    2.1 Dilute the TPE-MI stock solution to a working concentration of 50 μM with PBS.
    Note: Please adjust the concentration of the TPE-MI working solution according to the actual situation, and prepare it fresh for each use.
    3. Specific Staining Steps
    3.1 Cell Culture Preparation: Seed the cells in a suitable culture container.
    3.2 Washing: Wash cells with PBS.
    3.3 Staining: Add 50 μM TPE-MI working solution to the cells and incubate at 37 °C for 30 min.
    4. Detection
    Flow Cytometry Detection: Remove the TPE-MI solution, digest the cells, resuspend in PBS, centrifuge, discard the supernatant, and resuspend the cells in PBS for analysis.
    SDS-PAGE Detection: Lyse stained cells with cell lysis buffer, determine protein concentration, and perform SDS-PAGE electrophoresis followed by fluorescence and Coomassie Brilliant Blue staining analysis.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TPE-MI Related Antibodies
    Molecular Weight

    441.52

    Formula

    C31H23NO2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    Emission (Em)

    470
    Excitation (Ex)

    350
    SMILES

    O=C(C=C1)N(C2=CC=C(/C(C3=CC=CC=C3)=C(C4=CC=C(C)C=C4)/C5=CC=CC=C5)C=C2)C1=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 8.57 mg/mL (19.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2649 mL 11.3245 mL 22.6490 mL
    5 mM 0.4530 mL 2.2649 mL 4.5298 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2649 mL 11.3245 mL 22.6490 mL 56.6226 mL
    5 mM 0.4530 mL 2.2649 mL 4.5298 mL 11.3245 mL
    10 mM 0.2265 mL 1.1325 mL 2.2649 mL 5.6623 mL
    15 mM 0.1510 mL 0.7550 mL 1.5099 mL 3.7748 mL
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    TPE-MI Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TPE-MI1245606-71-6Tetraphenylethene maleimideHuntingtinParasiteunfolded proteinfluorescent probeHttex1HeLa cellsmalaria parasitesInhibitorinhibitorinhibit

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