YW1128
Based on 1 Customer Validation
YW1128 (compound 3a) is a potent Wnt/β-Catenin inhibitor. YW1128 induces the proteasome degradation of β-catenin and subsequent inhibits the Wnt/β-catenin signaling in cells. YW1128 significantly decreases hepatic lipid accumulation. YW1128 improves glucose tolerance of high fat diet-fed mice without noticeable toxicity. YW1128 down regulates the genes involved in the glucose and fatty acid anabolism.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.38%
- CAS. Nr.: 2131223-64-6
- Formel: C20H17N5O
- Molecular Weight:343.38
-
Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biologische Aktivität
-
Chemical Information
-
Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
-
Help & FAQs
Biologische Aktivität
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
20 nM
Compound: 3a
|
Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring siRNA after 24 hrs by luciferase reporter gene assay
Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring siRNA after 24 hrs by luciferase reporter gene assay
|
[PMID: 30605343] |
| HEK293 | IC50 |
34 nM
Compound: 3a
|
Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring GSK3B siRNA after 24 hrs by luciferase reporter gene assay
Inhibition of Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring GSK3B siRNA after 24 hrs by luciferase reporter gene assay
|
[PMID: 30605343] |
| HEK293 | IC50 |
4.1 nM
Compound: 3a
|
Inhibition of Wnt3a activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by Wnt3a addition and measured after 24 hrs by luciferase reporter gene assay
Inhibition of Wnt3a activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by Wnt3a addition and measured after 24 hrs by luciferase reporter gene assay
|
[PMID: 30605343] |
| HEK293 | IC50 |
6 nM
Compound: 3a
|
Inhibition of LiCl activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by LiCl addition and measured after 24 hrs by luciferase reporter gene assay
Inhibition of LiCl activated Wnt/beta-catenin signaling (unknown origin) expressed in HEK293 cells harboring TCF/LEF-responsive luciferase preincubated with cells followed by LiCl addition and measured after 24 hrs by luciferase reporter gene assay
|
[PMID: 30605343] |
| K562 | IC50 |
>50 μM
Compound: 1
|
Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
Anti-leukemia activity in human K562 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
|
[PMID: 35777269] |
Chemical Information
-
CAS. Nr. 2131223-64-6
-
Appearance Solid
-
Molecular Weight 343.38
-
Formel C20H17N5O
-
Color White to off-white
-
SMILES
O=C(C1=C(C)N(C2=CC=CC=C2C)N=N1)NC3=NC4=CC=CC=C4C=C3
-
Versand
Room temperature in continental US; may vary elsewhere.
-
Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (291.22 mM; ultrasonic and warming and heat to 160°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
-
Data Sheet (272 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9122 mL | 14.5611 mL | 29.1223 mL | 72.8056 mL |
| 5 mM | 0.5824 mL | 2.9122 mL | 5.8245 mL | 14.5611 mL | |
| 10 mM | 0.2912 mL | 1.4561 mL | 2.9122 mL | 7.2806 mL | |
| 15 mM | 0.1941 mL | 0.9707 mL | 1.9415 mL | 4.8537 mL | |
| 20 mM | 0.1456 mL | 0.7281 mL | 1.4561 mL | 3.6403 mL | |
| 25 mM | 0.1165 mL | 0.5824 mL | 1.1649 mL | 2.9122 mL | |
| 30 mM | 0.0971 mL | 0.4854 mL | 0.9707 mL | 2.4269 mL | |
| 40 mM | 0.0728 mL | 0.3640 mL | 0.7281 mL | 1.8201 mL | |
| 50 mM | 0.0582 mL | 0.2912 mL | 0.5824 mL | 1.4561 mL | |
| 60 mM | 0.0485 mL | 0.2427 mL | 0.4854 mL | 1.2134 mL | |
| 80 mM | 0.0364 mL | 0.1820 mL | 0.3640 mL | 0.9101 mL | |
| 100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.7281 mL |
YW1128 Related Classifications
HY-133180 Related Classifications
Powered by Bioz