2,4-D
Based on 1 publication(s) in Google Scholar
2,4-D (2, 4-dichlorophenoxyacetic acid) is a selective herbicide that can be used for the control of broad-leaved weeds. 2,4-D can induce apoptosis. 2,4-D inhibits DNA and protein synthesis, thereby preventing normal plant growth and development.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 94-75-7
- Formula: C8H6Cl2O3
- Molecular Weight:221.04
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 2,4-D
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
2, 4-D (25-200 μM, 72 h) inhibits the cell viability of A549 and WI38 with IC50 values of 126 µM and 115 µM, respectively. Induces apoptosis by influencing the cell cycle[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549, W138
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Concentration:25, 50, 75, 100, 150, 200 μM
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Incubation Time:72 h
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Result:Inhibited cell viability in a dose-dependent manner.
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Cell Line:A549
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Concentration:50, 100, 150 μM
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Incubation Time:72 h
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Result:The G0/G1 population decreased to about 57%, 43% and 31%, respectively.
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Cell Line:A549, W138
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Concentration:100, 150 μM
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Incubation Time:72 h
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Result:The early apoptotic population increased to 44% in A549 cells and 57% in WI38 cells, and the late apoptotic population increased to 18% in A549 cells and 10% in WI38 cells.
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Cell Line:A549, W138
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Concentration:100, 150 μM
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Incubation Time:72 h
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Result:Up-regulated p53 and Bax proteins, down-regulated Bcl-2 and procaspase 3.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult Wistar male rats[3]
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Dosage:100 or 200 mg/kg
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Administration:i.g.
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Result:Decreased the testis, seminal vesicles and prostate relative weight. Decreased the number of spermatozoa and sperm motility. Increased the level of FSH and LH.
Chemical Information
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CAS No. 94-75-7
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Appearance Solid
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Molecular Weight 221.04
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Formula C8H6Cl2O3
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Color White to off-white
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SMILES
O=C(O)COC1=CC=C(Cl)C=C1Cl
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Synonyms
2,4-Dichlorophenoxyacetic acid
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576
Solvent & Solubility
DMSO : 100 mg/mL (452.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.31 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (701 KB)
- English - EN (701 KB)
- Français - FR (701 KB)
- Deutsch - DE (701 KB)
- Norwegian - NO (701 KB)
- Español - ES (701 KB)
- Swedish - SV (701 KB)
- Italian - IT (701 KB)
- Portuguese - PT (701 KB)
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Handling Instructions (2659 KB)
References
[1]. Germaine KJ, et al. Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid. FEMS Microbiol Ecol. 2006 Aug;57(2):302-10. [Content Brief]
[3]. Marouani N, et al. Effects of oral administration of 2,4-dichlorophenoxyacetic acid (2,4-D) on reproductive parameters in male Wistar rats. Environ Sci Pollut Res Int. 2017 Jan;24(1):519-526. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.5241 mL | 22.6203 mL | 45.2407 mL | 113.1017 mL |
| 5 mM | 0.9048 mL | 4.5241 mL | 9.0481 mL | 22.6203 mL | |
| 10 mM | 0.4524 mL | 2.2620 mL | 4.5241 mL | 11.3102 mL | |
| 15 mM | 0.3016 mL | 1.5080 mL | 3.0160 mL | 7.5401 mL | |
| 20 mM | 0.2262 mL | 1.1310 mL | 2.2620 mL | 5.6551 mL | |
| 25 mM | 0.1810 mL | 0.9048 mL | 1.8096 mL | 4.5241 mL | |
| 30 mM | 0.1508 mL | 0.7540 mL | 1.5080 mL | 3.7701 mL | |
| 40 mM | 0.1131 mL | 0.5655 mL | 1.1310 mL | 2.8275 mL | |
| 50 mM | 0.0905 mL | 0.4524 mL | 0.9048 mL | 2.2620 mL | |
| 60 mM | 0.0754 mL | 0.3770 mL | 0.7540 mL | 1.8850 mL | |
| 80 mM | 0.0566 mL | 0.2828 mL | 0.5655 mL | 1.4138 mL | |
| 100 mM | 0.0452 mL | 0.2262 mL | 0.4524 mL | 1.1310 mL |
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