Bid BH3 peptide
Bid BH3 peptide is a pro-apoptotic peptide derived from the Bid protein. Through its α-helical conformation, Bid BH3 peptide inserts into the hydrophobic grooves of anti-apoptotic Bcl-2 family proteins (Bcl-xl, Bcl-2, Bcl-w and Bax), competitively displaces and releases the pro-apoptotic proteins Bax/Bak, thereby initiating mitochondrial outer membrane permeabilization (MOMP) and the caspase cascade for apoptosis. Bid BH3 peptide can be used in cancer-related research.
For research use only. We do not sell to patients.
- CAS No.: 300349-78-4
- Formula: C74H127N29O24S
- Molecular Weight:1839.05
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Bid BH3 peptide (10 μM; 6 h) does not induce apoptosis in Jurkat E6.1 cells, while bivalent and pentavalent dextran-conjugated Bid BH3 peptide increase caspase-3 activity by 12-fold and 21-fold, respectively, following nucleofection[1].
Monomeric Bid BH3 peptide (10 μM) conjugated to nonaarginine does not induce apoptosis in Jurkat E6.1 cells, while bivalent and pentavalent dextran-conjugated Bid BH3 peptide with nonaarginine increase caspase-3 activity by 7-fold and 17-fold, respectively[1].
Pentavalent dextran-conjugated Bid BH3 peptide (10 μM; 8 h) induces early apoptosis in Jurkat E6.1 cells, as evidenced by Annexin-V staining and intact cell membrane integrity[1].
Bid BH3 peptide (0.05-0.07 mM) is released as an unfolded species from its complex with truncated human BCL-w when BCL-w binds to 5-6 mM DPC micelles, and the released peptide binds to DPC micelles at concentrations up to 50 mM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Jurkat E6.1 lymphoma cells
-
Concentration:10 μM
-
Incubation Time:6 h
-
Result:Induced no significant apoptosis (no measurable increase in caspase-3 activity; monomeric form).
Increased caspase-3 activity by 12-fold (bivalent dextran-conjugated form).
Increased caspase-3 activity by 21-fold (pentavalent dextran-conjugated form).
-
Cell Line:Jurkat E6.1 lymphoma cells
-
Concentration:10 μM
-
Incubation Time:8 h
-
Result:Induced strong Annexin-V staining (indicative of early apoptosis) with intact cell membranes (trypan blue excluded from cells; pentavalent dextran-conjugated form).
Chemical Information
-
CAS No. 300349-78-4
-
Molecular Weight 1839.05
-
Formula C74H127N29O24S
-
Sequence
Arg-Asn-Ile-Ala-Arg-His-Leu-Ala-Gln-Val-Gly-Asp-Ser-Met-Asp-Arg
-
Sequence Shortening
RNIARHLAQVGDSMDR
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Richter M, et al. Multivalent design of apoptosis-inducing bid-BH3 peptide-oligosaccharides boosts the intracellular activity at identical overall peptide concentrations. Chemistry (Weinheim an der Bergstrasse, Germany). 2012 Dec 21;18(52):16708-15. [Content Brief]
[2]. Denisov AY, et al. Structural model of the BCL-w-BID peptide complex and its interactions with phospholipid micelles. Biochemistry. 2006 Feb 21;45(7):2250-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bid BH3 peptide Related Classifications
HY-P10614 Related Classifications
Powered by Bioz