Boserolimab
Based on 1 Customer Validation
Boserolimab (MK-5890) is a humanized agonist monoclonal antibody that binds to CD27 to provide a costimulatory signal that enhances T-cell–mediated responses.
For research use only. We do not sell to patients.
- Purity : 99.00%
- CAS No.: 2444297-08-7
- Molecular Weight:144.91 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
CD27
In Vitro
Boserolimab (0-20 µg/mL, 3 days) inhibits the binding of CD27 to CD70 with an IC50 of 0.24 nM, activates NF-κB signaling pathway, thereby promoting the activation of T cell, improving the cytokine (IFNγ) secretion[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse MC38 xenograft models[1]
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Dosage:5 mg/kg
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Administration:ip, 3 times weekly for 3-7 doses
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Result:Inhibited tumor growth.
Reduced the number of CD4+, CD8+ T cells and Treg cells in peripheral blood.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Human IgG1 kappa
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1X106 Raji cells with Boserolimab (HY-P990018, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Loaded CD27/TNFRSF7 Protein (HEK293,His-Fc, HY-P72742) on NTA biosensor, can bind Boserolimab with an affinity constant of 8.578E-10 M as determined in BLI assay.
Chemical Information
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CAS No. 2444297-08-7
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Appearance Liquid
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Molecular Weight 144.91 kDa
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Color Colorless to light yellow
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SMILES
[Boserolimab]
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Synonyms
MK-5890
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (231 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)