Box5

Name: Box5
Brand: MedChemExpress
SKU: HY-123071
Rating: 5.0 (11 reviews)

Cat. No.: HY-123071 Purity: 98.81%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Box5 is a potent Wnt5a antagonist. Box5 inhibits Wnt5a signaling and inhibits Wnt5a-mediated Ca²⁺ release. Box5 inhibits cell migration. Box5 has the potential for the research of melanoma.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 1206604-29-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Box5:

Box5 TFA In-stock

11 Publications Citing Use of MCE Box5

Cell Proliferation/Viability Assay

Cell Imaging/Staining

Histological Imaging/Staining

: Box5 purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2026 Feb 1:503:141085. [Abstract]; Protein level of p21, p16 and IL-6 after inhibiting Wnt5a with Box5 (100 μM; 24 h) in THLE-2 cells, and statistical analysis, α-Tubulin were used as control. The results showed that Box5 decreased the senescence related proteins.

: Box5 purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2026 Feb 1:503:141085. [Abstract]; Representative images of SA-β-Gal staining after inhibiting Wnt5a with Box5 (100 μM; 24 h) in THLE-2 cells. SA-β-Galactosidase staining confirmed that inhibition of Wnt5a decreased the senescence level of hepatocytes induced by PFPeA.

: Box5 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2024 Mar 26;21(1):75. [Abstract]; The cell viability of ARPE-19 cells examined with CCK-8 assay after treatment with the indicated doses of Box5 for 48 hours. The Vehicle Control groups for Box5 contained DMSO concentrations of 0.2% and 2%, respectively.

: Box5 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2024 Mar 26;21(1):75. [Abstract]; The protein levels of fibronectin, Wnt5a, Dvl2, and Naked1 in TGFβ1 (10 ng/mL)-treated ARPE-19 cells with or without different concentrations of Box5 (10, 45 and 90 μmol/L) for 48 h. Box5 suppressed the TGFβ1-mediated up-regulation of fibronectin, Wnt5a, Dvl2, and Naked1 in a dose-dependent manner.

: Box5 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2024 Mar 26;21(1):75. [Abstract]; The effects of intravitreal injections of FH535 or Box5 (90 μmol/L) on β-catenin and α-SMA expression within the CNV lesions in mice at day 21 after laser induction, along with the corresponding quantitative analysis. Box5 (90 μmol/L) treatment significantly reduced the immunostaining areas of active β-catenin by 47.39% (p = 0.00002, n = 10).

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Wnt Isoform Specific Products:

View All Isoforms

Wnt1 Wnt3 Wnt5 Wnt7 Wnt11

Biological Activity
Purity & Documentation
References
Customer Review

Description

Box5 is a potent Wnt5a antagonist. Box5 inhibits Wnt5a signaling and inhibits Wnt5a-mediated Ca²⁺ release. Box5 inhibits cell migration. Box5 has the potential for the research of melanoma^[1].

IC₅₀ & Target

Wnt5A

In Vitro

Box5 (100 μM) decreases the expression of rWnt5a (0.1 μg/mL) stimulated p-MARCKS in A2058 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	A2058 cells
Concentration:	100 μM
Incubation Time:	Overnight
Result:	Decreased the expression of rWnt5a (0.1 μg/mL) stimulated p-MARCKS.

Molecular Weight

766.88

Formula

C₃₀H₅₀N₆O₁₃S₂

CAS No.

1206604-29-6

Appearance

Solid

Color

White to off-white

Sequence

{t-Boc}-Met-Asp-Gly-Cys-Glu-Leu

Sequence Shortening

{t-Boc}-MDGCEL

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (130.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3040 mL	6.5199 mL	13.0399 mL
5 mM	0.2608 mL	1.3040 mL	2.6080 mL
10 mM	0.1304 mL	0.6520 mL	1.3040 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.81%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (247 KB) RP-HPLC (267 KB) MS (68 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)

References

[1]. Jenei V, et al. A t-butyloxycarbonyl-modified Wnt5a-derived hexapeptide functions as a potent antagonist of Wnt5a-dependent melanoma cell invasion. Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19473-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3040 mL	6.5199 mL	13.0398 mL	32.5996 mL
	5 mM	0.2608 mL	1.3040 mL	2.6080 mL	6.5199 mL
	10 mM	0.1304 mL	0.6520 mL	1.3040 mL	3.2600 mL
	15 mM	0.0869 mL	0.4347 mL	0.8693 mL	2.1733 mL
	20 mM	0.0652 mL	0.3260 mL	0.6520 mL	1.6300 mL
	25 mM	0.0522 mL	0.2608 mL	0.5216 mL	1.3040 mL
	30 mM	0.0435 mL	0.2173 mL	0.4347 mL	1.0867 mL
	40 mM	0.0326 mL	0.1630 mL	0.3260 mL	0.8150 mL
	50 mM	0.0261 mL	0.1304 mL	0.2608 mL	0.6520 mL
	60 mM	0.0217 mL	0.1087 mL	0.2173 mL	0.5433 mL
	80 mM	0.0163 mL	0.0815 mL	0.1630 mL	0.4075 mL
	100 mM	0.0130 mL	0.0652 mL	0.1304 mL	0.3260 mL

Box5 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Box51206604-29-6Box 5Box-5WntA2058 cellsrWnt5amelanomaanti-metastaticInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Box5

Customer Review

Other Forms of Box5:

Box5 Related Antibodies

11 Publications Citing Use of MCE Box5

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Wnt Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Box5 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Box5 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report