1. GPCR/G Protein
    TGF-beta/Smad
    Stem Cell/Wnt
  2. Prostaglandin Receptor
    TGF-beta/Smad
  3. Butaprost

Butaprost 

Cat. No.: HY-100448A Purity: ≥99.0%
Handling Instructions

Butaprost is a selective prostaglandin E receptor (EP2) agonist with an EC50 of 33 nM and a Ki of 2.4 μM for murine EP2 receptor. Butaprost is less activity against murine EP1, EP3 and EP4 receptors. Butaprost attenuates fibrosis by hampering TGF-β/Smad2 signalling.

For research use only. We do not sell to patients.

Butaprost Chemical Structure

Butaprost Chemical Structure

CAS No. : 69685-22-9

Size Price Stock Quantity
Solution
5 mg (12.24 mM * 1 mL in Methyl acetate) USD 1168 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Butaprost is a selective prostaglandin E receptor (EP2) agonist with an EC50 of 33 nM and a Ki of 2.4 μM for murine EP2 receptor. Butaprost is less activity against murine EP1, EP3 and EP4 receptors. Butaprost attenuates fibrosis by hampering TGF-β/Smad2 signalling[1][2][3].

IC50 & Target[3]

m-EP2 receptor

2400 nM (Ki)

m-EP2 receptor

33 nM (EC50)

In Vitro

Butaprost (1-100 nM; 0.5-24 hours) induces about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells in a dose- and time-dependent. Butaprost upregulated Nur77 gene expression through the PKC pathway[1].
Butaprost (50 μM; 24 hours) reduces TGF-β-induced fibronectin (FN) expression, Smad2 phosphorylation and epithelial-mesenchymal transition in Madin-Darby Canine Kidney (MDCK) cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HEK 293/EBNA cells stably expressing the human EP2 receptor (hEP2-HEK 293/EBNA cells)
Concentration: 1 nM, 10 nM, 100 nM
Incubation Time: 0.5 hours, 1 hours, 6 hours, 24 hours
Result: Induced about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells.
In Vivo

Butaprost (1-4 mg/kg; intraperitoneal injection; twice daily; for 7 days) treatment attenuates the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery, as illustrated by a reduction in the gene and protein expression of α-smooth muscle actin, fibronectin and collagen 1A1[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (8 weeks of age; 21 g) bearing unilateral ureteral obstruction surgery[2]
Dosage: 1 mg/kg, 2 mg/kg, 4 mg/kg
Administration: Intraperitoneal injection; twice daily; for 7 days
Result: Attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery.
Molecular Weight

408.57

Formula

C₂₄H₄₀O₅

CAS No.
SMILES

COC(CCCCCC[[email protected]@H](C(C[[email protected]]1O)=O)[[email protected]]1/C=C/C[[email protected]@H](C2(CCC2)CCC)O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

References

Purity: ≥99.0%

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

ButaprostProstaglandin ReceptorTGF-beta/SmadTransforming growth factor betaNur77EP2TGF-βSmad2fibronectinfibrogenesisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Butaprost
Cat. No.:
HY-100448A
Quantity:
MCE Japan Authorized Agent: