SRT6
Based on 1 Customer Validation
SRT6 is a CD44 inhibitor. SRT6 exerts antiproliferative activity in CD44+ breast cancer and lung cancer cells. SRT6 inhibits CD44-associated SRC kinase, as well as EGFR, ERBB2, ERBB4, MAP3K10 and MAPKAPK2. SRT6 can be used for the research of breast cancer and lung cancer.
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- Pureté : 99.90%
- Formule: C25H26N2O8S
- Masse moléculaire:514.55
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Stockage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
SRT6 (0.01-100 μM; 5 days) inhibits the proliferation of CD44+ MDA-MB-231, A549, and NCI-H23 cells, with EC50 values of 7.57, 5, and 1.37 μM, respectively[1].
The antiproliferative activity of SRT6 (7.57 μM; 5 days) against MDA-MB-231 breast cancer cells decreases by 13% after blocking CD44 with an anti-CD44 antibody, confirming that this activity is mediated by CD44[1].
SRT6 (5 μM-50 μM) potently inhibits CD44-associated SRC kinase, and also inhibits EGFR, ERBB2, ERBB4, MAP3K10 and MAPKAPK2 at a concentration of 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:CD44+ MDA-MB-231 human breast adenocarcinoma cells
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Concentration:0.01-100 μM
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Incubation Time:5 days
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Result:Inhibited MDA-MB-231 cell proliferation with an EC50 of 7.57 μM, as calculated from 10-point semilog dose-response curves.
Inhibited A549 cell proliferation with an EC50 of 5.00 μM, as calculated from 10-point semilog dose-response curves.
Inhibited NCI-H23 cell proliferation with an EC50 of 1.37 μM, as calculated from 10-point semilog dose-response curves.
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Cell Line:CD44+ MDA-MB-231 human breast adenocarcinoma cells
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Concentration:7.57 μM
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Incubation Time:5 days (SRT6 incubation); 30 min (anti-CD44 antibody pre-incubation)
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Result:Had its antiproliferative activity reduced by 13% compared to cells treated with SRT6 alone, confirming CD44 as a primary target.
Chemical Information
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Appearance Solid
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Masse moléculaire 514.55
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Formule C25H26N2O8S
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Color Off-white to light yellow
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SMILES
O=S(C1=CC=C([N+]([O-])=O)C=C1)(OC2=CC=CC3=C2CCN(CC4=CC(OC)=C(OC)C(OC)=C4)C3)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (194.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9434 mL | 9.7172 mL | 19.4345 mL | 48.5861 mL |
| 5 mM | 0.3887 mL | 1.9434 mL | 3.8869 mL | 9.7172 mL | |
| 10 mM | 0.1943 mL | 0.9717 mL | 1.9434 mL | 4.8586 mL | |
| 15 mM | 0.1296 mL | 0.6478 mL | 1.2956 mL | 3.2391 mL | |
| 20 mM | 0.0972 mL | 0.4859 mL | 0.9717 mL | 2.4293 mL | |
| 25 mM | 0.0777 mL | 0.3887 mL | 0.7774 mL | 1.9434 mL | |
| 30 mM | 0.0648 mL | 0.3239 mL | 0.6478 mL | 1.6195 mL | |
| 40 mM | 0.0486 mL | 0.2429 mL | 0.4859 mL | 1.2147 mL | |
| 50 mM | 0.0389 mL | 0.1943 mL | 0.3887 mL | 0.9717 mL | |
| 60 mM | 0.0324 mL | 0.1620 mL | 0.3239 mL | 0.8098 mL | |
| 80 mM | 0.0243 mL | 0.1215 mL | 0.2429 mL | 0.6073 mL | |
| 100 mM | 0.0194 mL | 0.0972 mL | 0.1943 mL | 0.4859 mL |
SRT6 Related Classifications
HY-175809 Related Classifications
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