2. PROTAC
  3. Molecular Glues Ligands for E3 Ligase
  4. CC-3060

CC-3060 is a molecular glue modulator targeting Cereblon (CRBN) E3 ubiquitin ligase and ZBTB16 degrader with a DC50 of 0.47 nM in HT-1080 cells. CC-3060 engages structural degrons on ZBTB16’s ZnF1 or ZnF3 domains for fusion protein substrates. CC-3060 can be used for the research of acute promyelocytic leukemia.

For research use only. We do not sell to patients.

CC-3060

CC-3060 Chemical Structure

CAS No. : 444288-86-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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CC-3060 Related Antibodies

Top Publications Citing Use of Products

View All Ligands for E3 Ligase Isoform Specific Products:

View All Isoforms
von Hippel-Lindau (VHL) MDM2 Cereblon IAP KLHDC2 Fbxo3 RNF4 DCAF11 DCAF1 UHRF1 TRIM21 NEURL1B KLHL41

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Description

CC-3060 is a molecular glue modulator targeting Cereblon (CRBN) E3 ubiquitin ligase and ZBTB16 degrader with a DC50 of 0.47 nM in HT-1080 cells. CC-3060 engages structural degrons on ZBTB16’s ZnF1 or ZnF3 domains for fusion protein substrates. CC-3060 can be used for the research of acute promyelocytic leukemia[1].

IC50 & Target[1]

Cereblon

 

In Vitro

CC-3060 (0.0001-10 μM; 6-16 h) potently and efficaciously induces CRBN- and proteasome-dependent degradation of wild-type ZBTB16 in HT-1080 ZBTB16-ePL cells, with a DC50 of 0.47 nM and a minimum remaining protein level of 28%[1].
CC-3060 (100 pM-10 μM; 6 h) drives CRBN- and proteasome-dependent degradation of endogenous ZBTB16 in HEL 92.1.7 cells, with complete degradation observed at 10 μM after 6 h[1].
CC-3060 (10 μM; 6 h) induces selective degradation of ZBTB16, IKZF1, ZFP91, and ZNF276 (but not GSPT1 or CSNK1A1) in KG-1a cells, with a significant log2 fold change of −1.73 for ZBTB16[1].
CC-3060 promotes CRL4CRBN-dependent ubiquitination of ZBTB16 ZnF1 and ZnF3 domains in vitro, with activity dependent on the critical glycine residues in each domain[1].
CC-3060 (0.0001-10 μM; 16 h) primarily utilizes the ZnF1 structural degron to promote ZBTB16 degradation in HT-1080 cells, with ZnF3 acting as a secondary, less efficient degron[1].
CC-3060 (0.0001-10 μM; 6-16 h) potently induces CRBN- and proteasome-dependent degradation of ZBTB16-RARα in HT-1080 cells via the ZnF1 structural degron, with a minimum remaining protein level of 20%[1].
CC-3060 stimulates CRL4CRBN-dependent ubiquitination of ZBTB16-RARα in vitro, with activity dependent on the critical glycine residue in the ZBTB16 ZnF1 domain[1].
CC-3060 (0.0001-10 μM; 6-16 h) induces CRBN- and proteasome-dependent degradation of RARα-ZBTB16 in HT-1080 cells via the ZnF3 structural degron, with a minimum remaining protein level of 77%[1].
CC-3060 stimulates CRL4CRBN-dependent ubiquitination of RARα-ZBTB16 in vitro, with activity dependent on the critical glycine residue in the ZBTB16 ZnF3 domain[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CC-3060 Related Antibodies

Western Blot Analysis[1]

Cell Line: human erythroleukemia HEL 92.1.7 cells (endogenous ZBTB16); CRBN knockout HEL 92.1.7 cells
Concentration: 100 pM; 1 nM; 10 nM; 100 nM; 1 μM; 10 μM
Incubation Time: 6 h
Result: Induced a dose-dependent decrease in endogenous ZBTB16 levels, with the highest tested concentration (10 μM) reducing ZBTB16 to 0% of DMSO control levels.
Exhibits blocked ZBTB16 degradation when cotreated with MLN4924 or MG132.
Shows completely suppressed downregulation of endogenous ZBTB16 in CRBN knockout cells.
Molecular Weight

403.39

Formula

C22H17N3O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC(C(CC1)N2C(C3=C(C2=O)C(NCC4=CC5=C(C=CC=C5)O4)=CC=C3)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (247.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4790 mL 12.3950 mL 24.7899 mL
5 mM 0.4958 mL 2.4790 mL 4.9580 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.7%

SDS (252 KB)

COA (256 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4790 mL 12.3950 mL 24.7899 mL 61.9748 mL
5 mM 0.4958 mL 2.4790 mL 4.9580 mL 12.3950 mL
10 mM 0.2479 mL 1.2395 mL 2.4790 mL 6.1975 mL
15 mM 0.1653 mL 0.8263 mL 1.6527 mL 4.1317 mL
20 mM 0.1239 mL 0.6197 mL 1.2395 mL 3.0987 mL
25 mM 0.0992 mL 0.4958 mL 0.9916 mL 2.4790 mL
30 mM 0.0826 mL 0.4132 mL 0.8263 mL 2.0658 mL
40 mM 0.0620 mL 0.3099 mL 0.6197 mL 1.5494 mL
50 mM 0.0496 mL 0.2479 mL 0.4958 mL 1.2395 mL
60 mM 0.0413 mL 0.2066 mL 0.4132 mL 1.0329 mL
80 mM 0.0310 mL 0.1549 mL 0.3099 mL 0.7747 mL
100 mM 0.0248 mL 0.1239 mL 0.2479 mL 0.6197 mL
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CC-3060 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CC-3060444288-86-2CC3060CC 3060Molecular GluesLigands for E3 LigaseE3 ligase-recruiting MoietyHT-1080 cellsIKZF1CRBNcereblonZBTB16-RARαZFP91acute promyelocytic leukemiaRARα-ZBTB16ZBTB16ZNF276Inhibitorinhibitorinhibit

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