ccc_R08
Based on 1 publication(s) in Google Scholar
ccc_R08 is a non-cytotoxic and orally active cccDNA inhibitor that reduces cccDNA levels in the liver of HBV-infected mice. ccc_R08 can be used in the study of HBV virus (hepatitis B virus) infection.
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- Purity: 98.87%
- CAS No.: 2919019-72-8
- 화학식: C22H19ClO6
- 분자량:414.84
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보관:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) ccc_R08
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
ccc_R08 (0.3, 1.0, 3.2, 10, 32 μM; 5 days) significantly reduces the level of cccDNA, protein-free RC-DNA, and double stranded linear DNA (DL-DNA) in HepDES19 cells[1].
ccc_R08 (0-100 μM) dose-dependently reduces the level of extracellular HBeAg from HepDES19 cells, with IC50 of ~0.1 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepDES19 cells
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Concentration:0.3, 1.0, 3.2, 10, 32 µM
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Incubation Time:5 days
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Result:Reduced the level of cccDNA, protein-free RC-DNA, and double stranded linear DNA (DL-DNA).
ccc_R08 (10, 15, 20, 30 mg/kg; p.o.; twice per day for 2 weeks) significantly decreases the serum level of pgRNA in a dose-dependent manner, and the reduction in pgRNA is quantitatively correlated with that in liver cccDNA at different doses[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HBVcircle mouse model [1].
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Dosage:20 mg/kg
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Administration:Oral administration; twice per day for 2 weeks
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Result:Led to the clearance of cccDNA from HBVcircle mouse livers.
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Animal Model:HBVcircle mouse model [1].
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Dosage:10, 15, 20, 30 mg/kg
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Administration:Oral administration; twice per day for 42 days
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Result:Led to a sustained reduction in the serum levels of pgRNA.
Chemical Information
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CAS No. 2919019-72-8
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Appearance Solid
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분자량 414.84
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화학식 C22H19ClO6
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Color White to yellow
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SMILES
O=C1C=C(C2=CC=C(OCCOC3CC(C(O)=O)C3)C=C2)OC4=C1C=CC=C4Cl
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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J Med Virol
Drug Repurposing: In Vitro Evaluation of Simeprevir as a Novel Antiviral Drug Against Severe Fever With Thrombocytopenia Syndrome Virus. [Abstract]2025 Oct;97(10):e70655. PMID: 41117261
용액&용해도
DMSO : 100 mg/mL (241.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.03 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4106 mL | 12.0528 mL | 24.1057 mL | 60.2642 mL |
| 5 mM | 0.4821 mL | 2.4106 mL | 4.8211 mL | 12.0528 mL | |
| 10 mM | 0.2411 mL | 1.2053 mL | 2.4106 mL | 6.0264 mL | |
| 15 mM | 0.1607 mL | 0.8035 mL | 1.6070 mL | 4.0176 mL | |
| 20 mM | 0.1205 mL | 0.6026 mL | 1.2053 mL | 3.0132 mL | |
| 25 mM | 0.0964 mL | 0.4821 mL | 0.9642 mL | 2.4106 mL | |
| 30 mM | 0.0804 mL | 0.4018 mL | 0.8035 mL | 2.0088 mL | |
| 40 mM | 0.0603 mL | 0.3013 mL | 0.6026 mL | 1.5066 mL | |
| 50 mM | 0.0482 mL | 0.2411 mL | 0.4821 mL | 1.2053 mL | |
| 60 mM | 0.0402 mL | 0.2009 mL | 0.4018 mL | 1.0044 mL | |
| 80 mM | 0.0301 mL | 0.1507 mL | 0.3013 mL | 0.7533 mL | |
| 100 mM | 0.0241 mL | 0.1205 mL | 0.2411 mL | 0.6026 mL |
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