Art. -Nr.: HY-120657

9-PAHSA

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9-PAHSA is an orally active endogenous GPR120 agonist (EC₅₀=18 μM). 9-PAHSA significantly inhibits LPS-induced inflammatory responses by blocking the NF-κB pathway. 9-PAHSA induces adipocyte browning, enhances glucose uptake and reduces lipid accumulation, while improving mitochondrial function and the survival rate of steatotic hepatocytes. In terms of neuroprotection, 9-PAHSA regulates the expression of REST and BDNF in the prefrontal cortex of diabetic mice, and effectively prevents spatial working memory deficits and abnormal social behaviors. 9-PAHSA does not directly regulate insulin secretion or improve systemic insulin sensitivity, and possesses specific anti-inflammatory, metabolic regulatory and neuroprotective properties. 9-PAHSA can be used in the research of diabetes-related cognitive impairment, obesity and non-alcoholic fatty liver disease.

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Reinheit: 99.88%
CAS. Nr.: 1481636-31-0
Formel: C₃₄H₆₆O₄
Molecular Weight:538.89

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

9-PAHSA Chemical Structure

CAS. Nr.: 1481636-31-0

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle NF-κB Isoform-spezifische Produkte anzeigen :

In Vitro

(R)-9-PAHSA and (S)-9-PAHSA (20 μM; 30 min pre-incubation) do not significantly increase basal or insulin-stimulated glucose uptake in human in vitro differentiated adipocytes^[1].
Racemic 9-PAHSA (1-20 μM; 2 h) does not stimulate glucose-dependent insulin secretion in dispersed mouse pancreatic islets^[1].
9-PAHSA (5-30 μM; 72 h) exhibits no significant cytotoxicity toward 3T3-L1 pre-adipocytes following 72 h of treatment^[3].
9-PAHSA (5-10 μM; 6 h) increases the viability of HepG2 cells with steatosis, with up to 140% relative viability observed in 500 μM oleic acid-treated cells, while higher 9-PAHSA concentrations reduce viability^[4].
9-PAHSA (10-40 μM; 6 h) reduces intracellular lipid accumulation in oleic acid-induced steatotic HepG2 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

HepG2
Concentration:

5 μM, 10 μM, 20 μM, 40 μM; tested in cells pre-treated with 250 μM, 500 μM, or 1000 μM oleic acid
Incubation Time:

6 h
Result:

Increased relative cell viability to up to 120% in HepG2 cells treated with 250 μM oleic acid at 5 μM, while reduced viability at 10 μM, 20 μM, and 40 μM.
Increased relative cell viability to up to 140% in HepG2 cells treated with 500 μM oleic acid at 5 μM and 10 μM, while reduced viability at 20 μM and 40 μM.
Increased relative cell viability slightly in HepG2 cells treated with 1000 μM oleic acid at 5 μM and 10 μM, while reduced viability at 20 μM and 40 μM.

In Vivo

9-PAHSA (22.5 mg/kg; oral gavage; single acute dose) does not improve glucose tolerance or stimulate insulin or GLP-1 secretion in male C57BL/6J mice fed VS-LFD or VS-HFD for 18 weeks^[1].
9-PAHSA (45 mg/kg; oral gavage; single acute dose) does not improve glucose tolerance or stimulate insulin or GLP-1 secretion in male C57BL/6J mice fed BT-HFD for 18 weeks^[1].
9-PAHSA (15 mg/kg; oral gavage; single acute dose) does not improve glucose tolerance or stimulate glucose-stimulated insulin secretion in male C57BL/6J mice fed LARD-HFD for 20 weeks^[1].
9-PAHSA (50 mg/kg; i.g.; daily; 28 days) improves type 2 diabetes-associated cognitive impairment in db/db mice by reducing cortical REST expression and upregulating cortical BDNF expression, while also restoring spatial working memory and social novelty preference^[2].
9-PAHSA (50 mg/kg; p.o.; once daily; 28 days) promotes browning of subcutaneous inguinal white adipose tissue in both wild-type and ob/ob mice via upregulation of brown fat-specific genes and proteins, with more pronounced effects in ob/ob mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J mice with Type 2 diabetes (male; 18-week diet-induced obesity via BT-HFD feeding)^[1]
Dosage:

45 mg/kg
Administration:

oral gavage; single acute dose
Result:

Increased plasma levels significantly to around 145 nM.
Detected no significant differences in glucose excursion during oGTT, baseline insulin secretion, glucose-stimulated insulin secretion, or active GLP-1 release compared to vehicle-treated mice.

Animal Model:

C57 mice with Obesity (20-week-old male, wild-type); C57BL ob/ob (20-week-old male, transgenic obesity model)^[3]
Dosage:

50 mg/kg
Administration:

p.o.; once daily; 28 days
Result:

Did not significantly alter body weight or food intake in either WT or ob/ob mice.
Reduced triglyceride levels (no specific numerical value provided).
Significantly increased mRNA expression of brown fat-specific markers UCP1, PGC1α, Cidea, and PRDM16 in sWAT, with greater increases observed in ob/ob mice compared to WT mice.
Significantly elevated protein levels of UCP1 and PGC1α in both mouse strains.
Induced smaller, multilocular fat cells in sWAT of treated mice via H&E staining.
Increased UCP1 and PGC1α staining in sWAT via immunohistochemistry.

Chemical Information

CAS. Nr. 1481636-31-0
Appearance Solid
Molecular Weight 538.89
Formel C₃₄H₆₆O₄
Color White to off-white
SMILES

OC(CCCCCCCC(CCCCCCCCC)OC(CCCCCCCCCCCCCCC)=O)=O
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 5 mg/mL (9.28 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8557 mL

9.2783 mL

18.5567 mL

5 mM

0.3711 mL

1.8557 mL

3.7113 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 0.5 mg/mL (0.93 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 0.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 0.5 mg/mL (0.93 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 0.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 0.5 mg/mL (0.93 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 0.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (647 KB)
- English - EN (647 KB)
- Français - FR (647 KB)
- Deutsch - DE (647 KB)
- Norwegian - NO (647 KB)
- Español - ES (647 KB)
- Swedish - SV (647 KB)
- Italian - IT (647 KB)
- Korean - KR (647 KB)
- Portuguese - PT (647 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Pflimlin E, et al. Acute and Repeated Treatment with 5-PAHSA or 9-PAHSA Isomers Does Not Improve Glucose Control in Mice. Cell Metab. 2018;28(2):217-227.e13. [Content Brief]

[2]. Wen XH, et al. Effect of 9 - PAHSA on cognitive dysfunction in diabetic mice and its possible mechanism. Biochem Biophys Res Commun. 2020;524(3):525-532. [Content Brief]

[3]. Wang Y M, et al. 9-PAHSA promotes browning of white fat via activating G-protein-coupled receptor 120 and inhibiting lipopolysaccharide/NF-kappa B pathway[J]. Biochemical and biophysical research communications, 2018, 506(1): 153-160.

[4]. Schultz Moreira AR, et al. 9-PAHSA Prevents Mitochondrial Dysfunction and Increases the Viability of Steatotic Hepatocytes. Int J Mol Sci. 2020;21(21):8279. Published 2020 Nov 5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8557 mL	9.2783 mL	18.5567 mL	46.3917 mL
DMSO	5 mM	0.3711 mL	1.8557 mL	3.7113 mL	9.2783 mL

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9-PAHSA

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Neuronal Signaling Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

CNS-Penetrant Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Anti-diabetic Compound Library

Lipid Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Antidepressant Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Fatty Acids Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Alle NF-κB Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

9-PAHSA Related Classifications

HY-120657 Related Classifications