Dynasore
Based on 90 publication(s) in Google Scholar
Dynasore is a cell-permeable dynamin inhibitor with an IC50 of 15 μM. Dynasore blocks cell migration.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.77%
- CAS. Nr.: 304448-55-3
- Formel: C18H14N2O4
- Molecular Weight:322.31
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Publications Citing Use of MedChemExpress (MCE) Dynasore
More- Signal Transduct Target Ther. 2024 Sep 26;9(1):254. [Abstract]
- Science. 2025 Feb 21;387(6736):892-900. [Abstract]
- Nat Nanotechnol. 2020 Dec;15(12):1043-1052. [Abstract]
- Mol Cancer. 2026 Feb 10;25(1):73. [Abstract]
- Adv Mater. 2025 Jan 10:e2415030. [Abstract]
- Adv Mater. 2022 Jul 28;e2204287. [Abstract]
- Immunity. 2025 Apr 8:S1074-7613(25)00128-1. [Abstract]
- Sci Bull. 2020 Nov 15;65(21):1796-1799. [Abstract]
- Adv Funct Mater. 2024 May 1.
- Adv Funct Mater. 2023 Jan 15.
- Cell Host Microbe. 2025 Sep 30:S1931-3128(25)00375-0. [Abstract]
- Nat Commun. 2025 Nov 13;16(1):9973. [Abstract]
- Nat Commun. 2025 Aug 27;16(1):7994. [Abstract]
- Nat Commun. 2021 Jun 9;12(1):3481. [Abstract]
- J Am Chem Soc. 2018 Dec 12;140(49):17234-17240. [Abstract]
- Cell Death Differ. 2024 Nov;31(11):1519-1533. [Abstract]
- J Extracell Vesicles. 2025 Aug;14(8):e70146. [Abstract]
- J Extracell Vesicles. 2021 Jul;10(9):e12117. [Abstract]
- Autophagy. 2025 May 31:1-21. [Abstract]
- Adv Sci (Weinh). 2025 Dec 23:e17792. [Abstract]
- Adv Sci (Weinh). 2025 Sep 3:e02788. [Abstract]
- Adv Sci (Weinh). 2023 Feb;10(4):e2204871. [Abstract]
- Nat Chem Biol. 2026 Feb 13. [Abstract]
- Nat Plants. 2025 Sep 12. [Abstract]
- Biomaterials. 2025 Dec 18:329:123925. [Abstract]
- Biomaterials. 2022 May;284:121529. [Abstract]
- Redox Biol. 2025 May:82:103581. [Abstract]
- Redox Biol. 2022 Apr;50:102257. [Abstract]
- J Hazard Mater. 2026 Mar 1:505:141468. [Abstract]
- Environ Sci Technol. 2023 Jun 13;57(23):8514-8523. [Abstract]
- J Hazard Mater. 2021 Sep 5;417:126092. [Abstract]
- Alzheimers Dement. 2025 Aug;21(8):e70331. [Abstract]
- EBioMedicine. 2022 Aug:82:104181. [Abstract]
- Plant Biotechnol J. 2025 Aug;23(8):3237-3253. [Abstract]
- Int J Biol Sci. 2024 Sep 3;20(12):4713-4730. [Abstract]
- Food Chem. 2024 Sep 8;463(Pt 1):141196. [Abstract]
- Adv Healthc Mater. 2024 Jul 16:e2401460. [Abstract]
- Sci China Life Sci. 2022 Feb;65(2):341-361. [Abstract]
- Genes Dis. 2025 Aug 14.
- Small Methods. 2021 Apr;5(4):e2001047. [Abstract]
- Nano Lett. 2019 Nov 13;19(11):8010-8020. [Abstract]
- Cell Commun Signal. 2020 Nov 4;18(1):176. [Abstract]
- Int J Biol Macromol. 2022 Aug 31:215:23-35. [Abstract]
- Cancer Immunol Res. 2022 Jul 1;10(7):844-855. [Abstract]
- Drug Deliv. 2018 Nov;25(1):1066-1079. [Abstract]
- Mov Disord. 2024 Jan;39(1):40-52. [Abstract]
- Oncogene. 2018 Jan 4;37(1):116-127. [Abstract]
- Cell Rep. 2022 Jun 14;39(11):110943. [Abstract]
- Anal Chem. 2020 Jan 21;92(2):2103-2111. [Abstract]
- Antioxidants (Basel). 2022 Nov 22;11(12):2307. [Abstract]
- Elife. 2022 Jun 28;11:e78044. [Abstract]
- EMBO Rep. 2025 Sep 15. [Abstract]
- Environ Health. 2025 Nov 6.
- Int J Pharm. 2024 Apr 25:655:124072. [Abstract]
- Arab J Chem. 2020, 13 (1): 2558-2567.
- Int J Mol Sci. 2025 Mar 29;26(7):3172. [Abstract]
- PLoS Pathog. 2021 Jul 21;17(7):e1009752. [Abstract]
- ACS Appl Polym Mater. 2026 Feb 25.
- Int Immunopharmacol. 2025 Sep 23:162:115167. [Abstract]
- Eur J Pharmacol. 2021 Sep 15:907:174203. [Abstract]
- Biol Res. 2023 Mar 11;56(1):10. [Abstract]
- Front Cell Dev Biol. 2021 Jul 7;9:687322. [Abstract]
- Eur J Cell Biol. 2019 Jan;98(1):36-50. [Abstract]
- FEBS J. 2025 Mar;292(5):1052-1069. [Abstract]
- Comput Struct Biotechnol J. 2021;19:1933-1943. [Abstract]
- Toxins. 2023 Feb 6;15(2):132. [Abstract]
- AAPS PharmSciTech. 2023 Feb 15;24(2):69. [Abstract]
- Sci Rep. 2023 May 25;13(1):8479. [Abstract]
- J Virol. 2025 Aug 25:e0022125. [Abstract]
- J Virol. 2021 Nov 23;95(24):e0134521. [Abstract]
- Neurotox Res. 2019 Jul;36(1):108-116. [Abstract]
- J Cancer. 2025 Jan 27;16(5):1466-1478. [Abstract]
- Virus Res. 2020 Jan 15;276:197806. [Abstract]
- Virology. 2024 Dec:600:110233. [Abstract]
- Northwestern University. 2026.
- Icahn School of Medicine at Mount Sinai. 2026.
- Cell Biomater. 2026 Mar 19.
- bioRxiv. 2026 Mar 12.
- bioRxiv. 2026 Mar 17:2026.03.13.711603. [Abstract]
- Res Sq. 2026 Feb 5.
- bioRxiv. 2026 Jan 9.
- SSRN. 2025 Sep 30.
- bioRxiv. 2025 Oct 24:2025.10.24.684008. [Abstract]
- bioRxiv. 2025 Jul 29.
- SSRN. 2023 Sep 15.
- Research Square Preprint. 2023 Aug 7.
- Research Square Preprint. 2023 Apr 19.
- Research Square Print. 2022 May.
- SSRN. 14 Feb 2022.
- Research Square Preprint. 2021 Aug.
Customer Validation & Images
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Flow Cytometry
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IF
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IP
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WB
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Cell Imaging/Staining
Biologische Aktivität
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HSV-1 |
HSV-2 |
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Cell Line
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Type | Value | Description | References |
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| COS-7 | IC50 |
15 μM
Compound: DD-4, Dynasore
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Inhibition of transferrin uptake in african green monkey COS7 cells by microscopic TexasRed imaging
Inhibition of transferrin uptake in african green monkey COS7 cells by microscopic TexasRed imaging
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[PMID: 20621477] |
Dynasore interferes with the GTPase activity of dynamin1, dynamin2, and Drp1, the mitochondrial dynamin, but not of other small GTPases. Dynasore acts as a potent inhibitor of endocytic pathways known to depend on dynamin by rapidly blocking coated vesicle formation within seconds of dynasore addition. Two types of coated pit intermediates accumulate during dynasore treatment, g-shaped, half formed pits and O-shaped, fully formed pits, captured while pinching off[1]. Dynasore inhibits HSV-1 and HSV-2 infection of human epithelial and neuronal cells, including primary genital tract cells and human fetal neurons and astrocytes. Dynasore reduces the number of viral capsids reaching the nuclear pore if added at the time of viral entry and that, when added as late as 8 h postentry, dynasore blocks the transport of newly synthesized viral proteins from the nucleus to the cytosol[2]. Dynasore prevents ischemia/reperfusion induced elevation of left ventricular end diastolic pressure. Dynasore also decreases cardiac troponin I efflux during reperfusion and reduces infarct size. In cultured adult mouse cardiomyocytes subjected to oxidative stress, dynasore increases cardiomyocyte survival and viability[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 304448-55-3
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Appearance Solid
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Molecular Weight 322.31
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Formel C18H14N2O4
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Color Light brown to brown
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SMILES
O=C(C1=C(O)C=C2C=CC=CC2=C1)N/N=C/C3=CC=C(O)C(O)=C3
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (90)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Palmitoylation regulates myelination by modulating the ZDHHC3-Cadm4 axis in the central nervous system. [Abstract]2024 Sep 26;9(1):254. PMID: 39327467
Dynasore purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):254. [Abstract]
N2a cells expressing Cadm4 or Cadm4-C347A were treated with Dynasore(80 μM, 12 h) for preparing plasma membrane fractions, which were evaluated by WB.
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Science
2025 Feb 21;387(6736):892-900. PMID: 39977508 -
Nat Nanotechnol
A general strategy towards personalized nanovaccines based on fluoropolymers for post-surgical cancer immunotherapy. [Abstract]2020 Dec;15(12):1043-1052. PMID: 33139933 -
Mol Cancer
Tumour-infiltrating microplastics disrupt the JAK-STAT-microbiota axis to promote immunotherapy resistance in colorectal cancer. [Abstract]2026 Feb 10;25(1):73. PMID: 41664174 -
Adv Mater
Self-Sustained Biophotocatalytic Nano-Organelle Reactors with Programmable DNA Switches for Combating Tumor Metastasis. [Abstract]2025 Jan 10:e2415030. PMID: 39797479
Dynasore purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030. [Abstract]
Dynasore （50 µg/mL， 7 h）. FCM analysis of F/G/H‐GNC internalization by 4T1 cells pre‐treated with various endocytosis inhibitors (n = 3).
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Adv Mater
A Tetrahedral Framework DNA-Based Bioswitchable miRNA Inhibitor Delivery System: Application to Skin Anti-Aging. [Abstract]2022 Jul 28;e2204287. PMID: 35901292 -
Immunity
Alcaligenes faecalis induces intestinal T helper-17 cells by enhancing Rorc transcription through E3 ligase Trim21-mediated Fbxw7 degradation. [Abstract]2025 Apr 8:S1074-7613(25)00128-1. PMID: 40215968 -
Sci Bull
Dyngo-4a protects mice from rotavirus infection by affecting the formation of dynamin 2 oligomers. [Abstract]2020 Nov 15;65(21):1796-1799. PMID: 36659118 -
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Cell Host Microbe
Immunometabolic reprogramming of macrophages by gut microbiota-derived cadaverine controls colon inflammation. [Abstract]2025 Sep 30:S1931-3128(25)00375-0. PMID: 41033313 -
Nat Commun
Heat shock protein DNAJA2 controls insulin signaling and glucose homeostasis by preventing spontaneous insulin receptor endocytosis. [Abstract]2025 Nov 13;16(1):9973. PMID: 41233317
Dynasore purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 13;16(1):9973. [Abstract]
Immunofluorescence (IF) analysis of GFP-tagged IR (green) and RAB7 (red) in HepG2 cells transfected with various siRNAs or treated with dynasore (50 μM for 4 h) as indicated.
Dynasore purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 13;16(1):9973. [Abstract]
Co-immunoprecipitation (co-IP) assay showing the interaction between IRβ and AP2B1 in WT and DJ2−/− 4T1 cells treated with 50 μM dynasore for 4 h. The red arrow indicates DNAJA2 band.
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Nat Commun
Microglial replacement in a Sandhoff disease mouse model reveals myeloid-derived β-hexosaminidase is necessary for neuronal health. [Abstract]2025 Aug 27;16(1):7994. PMID: 40866328 -
Nat Commun
TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4. [Abstract]2021 Jun 9;12(1):3481. PMID: 34108482 -
J Am Chem Soc
2018 Dec 12;140(49):17234-17240. PMID: 30398334
Dynasore purchased from MedChemExpress. Usage Cited in: J Am Chem Soc. 2018 Dec 12;140(49):17234-17240. [Abstract]
HEK 293 cells are treated with different concentrations of Dynasore for indicated time. Then, the treated cells are collected and Western blot analysis is performed with indicated antibodies to detect LC3-I and LC3-II. GAPDH serves as the loading control.
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Cell Death Differ
Vps34 sustains Treg cell survival and function via regulating intracellular redox homeostasis. [Abstract]2024 Nov;31(11):1519-1533. PMID: 39117783 -
J Extracell Vesicles
Exomeres From Adventitial Fibroblasts of Spontaneously Hypertensive Rats Promote Vascular Remodelling via Transferring Osteopontin. [Abstract]2025 Aug;14(8):e70146. PMID: 40767027 -
J Extracell Vesicles
Extracellular vesicle-mediated endothelial apoptosis and EV-associated proteins correlate with COVID-19 disease severity. [Abstract]2021 Jul;10(9):e12117. PMID: 34262673
Dynasore purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2021 Jul;10(9):e12117. [Abstract]
Fluorescence images showing uptake of labelled SEVs by HPMECs in the presence or absence of the EV uptake inhibitor Dynasore (10 μM, 30 min).
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Autophagy
2025 May 31:1-21. PMID: 40394978 -
Adv Sci (Weinh)
Biomimetic Nanomedicine for Senescence-Modulated Immune Activation Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma. [Abstract]2025 Dec 23:e17792. PMID: 41432006 -
Adv Sci (Weinh)
Physiological pH Transition-Driven Protein Corona Dynamics Regulate Cellular Uptake and Inflammatory Responses of Silica Nanoparticles. [Abstract]2025 Sep 3:e02788. PMID: 40902197 -
Adv Sci (Weinh)
2023 Feb;10(4):e2204871. PMID: 36507570 -
Nat Chem Biol
2026 Feb 13. PMID: 41688711 -
Nat Plants
An apoplastic fungal effector disrupts N-glycosylation of ZmLecRK1, inducing its degradation to suppress disease resistance in maize. [Abstract]2025 Sep 12. PMID: 40940425 -
Biomaterials
2025 Dec 18:329:123925. PMID: 41453350 -
Biomaterials
Targeted immunomodulation therapy for cardiac repair by platelet membrane engineering extracellular vesicles via hitching peripheral monocytes. [Abstract]2022 May;284:121529. PMID: 35447403 -
Redox Biol
AGR2-mediated cell-cell communication controls the antiviral immune response by promoting the thiol oxidation of TRAF3. [Abstract]2025 May:82:103581. PMID: 40085973 -
Redox Biol
T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2. [Abstract]2022 Apr;50:102257. PMID: 35149342 -
J Hazard Mater
Mechanism of macrophage mitochondrial transfer in CBNPs induced EndMT of pulmonary microvascular endothelial cells. [Abstract]2026 Mar 1:505:141468. PMID: 41702162 -
Environ Sci Technol
2023 Jun 13;57(23):8514-8523. PMID: 37252706 -
J Hazard Mater
Systematic toxicity evaluation of polystyrene nanoplastics on mice and molecular mechanism investigation about their internalization into Caco-2 cells. [Abstract]2021 Sep 5;417:126092. PMID: 34015712 -
Alzheimers Dement
Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning. [Abstract]2025 Aug;21(8):e70331. PMID: 40731189 -
EBioMedicine
Intestinal epithelial β Klotho is a critical protective factor in alcohol-induced intestinal barrier dysfunction and liver injury. [Abstract]2022 Aug:82:104181. PMID: 35908416 -
Plant Biotechnol J
RabE1a- and SEC10b-mediated exocytosis and AP2β-mediated endocytosis are involved in the intracellular transport of tobamoviruses. [Abstract]2025 Aug;23(8):3237-3253. PMID: 40408557 -
Int J Biol Sci
Neutrophil extracellular traps trigger alveolar epithelial cell necroptosis through the cGAS-STING pathway during acute lung injury in mice. [Abstract]2024 Sep 3;20(12):4713-4730. PMID: 39309425 -
Food Chem
Absorption and transport mechanism of colloidal nanoparticles (CNPs) in lamb soup based on Caco-2 cell. [Abstract]2024 Sep 8;463(Pt 1):141196. PMID: 39260179 -
Adv Healthc Mater
Enhanced Tissue Regeneration Through Immunomodulatory Extracellular Vesicles Encapsulated in Diversified Silk-Based Injectable Hydrogels. [Abstract]2024 Jul 16:e2401460. PMID: 39011805 -
Sci China Life Sci
2022 Feb;65(2):341-361. PMID: 34047913 -
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Small Methods
2021 Apr;5(4):e2001047. PMID: 34927842 -
Nano Lett
2019 Nov 13;19(11):8010-8020. PMID: 31639306 -
Cell Commun Signal
Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor. [Abstract]2020 Nov 4;18(1):176. PMID: 33148272 -
Int J Biol Macromol
Investigation of the internalization and transport mechanism of Codonopsis Radix polysaccharide both in mice and Caco-2 cells. [Abstract]2022 Aug 31:215:23-35. PMID: 35718143 -
Cancer Immunol Res
High Mobility Group Protein B1 Decreases Surface Localization of PD-1 to Augment T-cell Activation. [Abstract]2022 Jul 1;10(7):844-855. PMID: 35580259 -
Drug Deliv
Bispecific antibodies (anti-mPEG/anti-HER2) for active tumor targeting of docetaxel (DTX)-loaded mPEGylated nanocarriers to enhance the chemotherapeutic efficacy of HER2-overexpressing tumors. [Abstract]2018 Nov;25(1):1066-1079. PMID: 29718725 -
Mov Disord
Erythrocytic α-Synuclein and the Gut Microbiome: Kindling of the Gut-Brain Axis in Parkinson's Disease. [Abstract]2024 Jan;39(1):40-52. PMID: 37798868 -
Oncogene
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) promotes EGF receptor signaling of oral squamous cell carcinoma metastasis via the complex N-glycosylation. [Abstract]2018 Jan 4;37(1):116-127. PMID: 28892050 -
Cell Rep
Vps33B controls Treg cell suppressive function through inhibiting lysosomal nutrient sensing complex-mediated mTORC1 activation. [Abstract]2022 Jun 14;39(11):110943. PMID: 35705052 -
Anal Chem
Chemoenzymatic Labeling of Extracellular Vesicles for Visualizing Their Cellular Internalization in Real Time. [Abstract]2020 Jan 21;92(2):2103-2111. PMID: 31876137 -
Antioxidants (Basel)
Oxybaphus himalaicus Mitigates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting TLR4/MD2 Complex Formation. [Abstract]2022 Nov 22;11(12):2307. PMID: 36552516 -
Elife
Domain fusion TLR2-4 enhances the autophagy-dependent clearance of Staphylococcus aureus in the genetic engineering goat. [Abstract]2022 Jun 28;11:e78044. PMID: 35762728 -
EMBO Rep
CDC42 supports HBV entry by NTCP translocation to the plasma membrane and macropinocytosis. [Abstract]2025 Sep 15. PMID: 40954218 -
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Int J Pharm
Integration of caveolin-mediated cytosolic delivery and enzyme-responsive releasing of squalenoyl nanoparticles enhance the anti-cancer efficacy of chidamide in pancreatic cancer. [Abstract]2024 Apr 25:655:124072. PMID: 38561133 -
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Int J Mol Sci
D-M159 Synergistically Induces Apoptosis in HeLa Cells Through Endoplasmic Reticulum Stress and Mitochondrial Dysfunction. [Abstract]2025 Mar 29;26(7):3172. PMID: 40243937 -
PLoS Pathog
Pore-forming alpha-hemolysin efficiently improves the immunogenicity and protective efficacy of protein antigens. [Abstract]2021 Jul 21;17(7):e1009752. PMID: 34288976 -
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Int Immunopharmacol
2025 Sep 23:162:115167. PMID: 40614595 -
Eur J Pharmacol
Biased agonists with less glucagon-like peptide-1 receptor-mediated endocytosis prolong hypoglycaemic effects. [Abstract]2021 Sep 15:907:174203. PMID: 34048741 -
Biol Res
DYRK1-mediated phosphorylation of endocytic components is required for extracellular lumen expansion in ascidian notochord. [Abstract]2023 Mar 11;56(1):10. PMID: 36899423 -
Front Cell Dev Biol
CD44v6-O-MWNTS-Loaded Gemcitabine and CXCR4 siRNA Improves the Anti-tumor Effectiveness of Ovarian Cancer. [Abstract]2021 Jul 7;9:687322. PMID: 34307366 -
Eur J Cell Biol
Overexpression of Toll-like receptor 4 enhances LPS-induced inflammatory response and inhibits Salmonella Typhimurium growth in ovine macrophages. [Abstract]2019 Jan;98(1):36-50. PMID: 30522781 -
FEBS J
A high cholesterol diet aggravates experimental colitis through SREBP2-modulated endocytosis and degradation of occludin and Zo-1 proteins. [Abstract]2025 Mar;292(5):1052-1069. PMID: 39279038 -
Comput Struct Biotechnol J
Dependence of SARS-CoV-2 infection on cholesterol-rich lipid raft and endosomal acidification. [Abstract]2021;19:1933-1943. PMID: 33850607 -
Toxins
Absorption and Transport Mechanism of Red Meat-Derived N-glycolylneuraminic Acid and Its Damage to Intestinal Barrier Function through the NF-κB Signaling Pathway. [Abstract]2023 Feb 6;15(2):132. PMID: 36828446 -
AAPS PharmSciTech
Transcellular Transport Behavior of the Intact Polymeric Mixed Micelles with Different Polymeric Ratios. [Abstract]2023 Feb 15;24(2):69. PMID: 36792796 -
Sci Rep
Rab27a-mediated extracellular vesicle secretion contributes to osteogenesis in periodontal ligament-bone niche communication. [Abstract]2023 May 25;13(1):8479. PMID: 37231020 -
J Virol
SFTSV utilizes AXL/GAS6 for entry via PI3K-PLC-dependent macropinocytosis activated by AXL-kinase. [Abstract]2025 Aug 25:e0022125. PMID: 40853130 -
J Virol
Porcine Deltacoronavirus Enters Porcine IPI-2I Intestinal Epithelial Cells via Macropinocytosis and Clathrin-Mediated Endocytosis Dependent on pH and Dynamin. [Abstract]2021 Nov 23;95(24):e0134521. PMID: 34586858 -
Neurotox Res
Dynasore Suppresses mTORC1 Activity and Induces Autophagy to Regulate the Clearance of Protein Aggregates in Neurodegenerative Diseases. [Abstract]2019 Jul;36(1):108-116. PMID: 30924108 -
J Cancer
Tumor cell-derived hyaluronan fragments induce endocytosis of S1PR1 to promote lymphangiogenesis through LYVE-1-Src pathway. [Abstract]2025 Jan 27;16(5):1466-1478. PMID: 39991586 -
Virus Res
Muscovy duck reovirus enters susceptible cells via a caveolae-mediated endocytosis-like pathway. [Abstract]2020 Jan 15;276:197806. PMID: 31704247 -
Virology
2024 Dec:600:110233. PMID: 39255726 -
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bioRxiv
Ischemia-Induced Post-Translational Modifications of GLT-1 Mediate Aberrant Trafficking and Impaired Glutamate Uptake. [Abstract]2026 Mar 17:2026.03.13.711603. PMID: 41890068 -
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bioRxiv
Ribosome remodeling drives translation adaptation during viral infection and cellular stress. [Abstract]2025 Oct 24:2025.10.24.684008. PMID: 41279401 -
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Lösungsmittel & Löslichkeit
DMSO : 50 mg/mL (155.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
Mouse ventricular myocytes are isolated from male adult C6/Black mouse. Cardiomyocytes subjects to 2 hours of drug treatment followed by oxidative stress (30 µM H2O2 for 35 min). For ATP supplement experiments, the cells are treated with 3 mM ATP for 30 min before exposure to H2O2. Cardiomyocyte survival and viability are analyzed by trypan blue exclusion (TBE) assay[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats: In the dynasore groups, the rats are given dynasore immediately at a dose of 1, 10, or 30 mg/kg through intraperitoneal injection after SCI, while the rats in the sham and SCI groups receive DMSO (same volume as dynasore groups) through intraperitoneal injection[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (758 KB)
- English - EN (758 KB)
- Français - FR (758 KB)
- Deutsch - DE (758 KB)
- Norwegian - NO (758 KB)
- Español - ES (758 KB)
- Swedish - SV (758 KB)
- Italian - IT (758 KB)
- Portuguese - PT (758 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Macia E, et al. Dynasore, a cell-permeable inhibitor of dynamin. Dev Cell. 2006 Jun;10(6):839-50. [Content Brief]
[2]. Mues MB, et al. Dynasore disrupts trafficking of herpes simplex virus proteins. J Virol. 2015 Jul;89(13):6673-84. [Content Brief]
[3]. Gao D, et al. Dynasore protects mitochondria and improves cardiac lusitropy in Langendorff perfused mouse heart. PLoS One. 2013 Apr 15;8(4):e60967. [Content Brief]
[4]. Li G, et al. Dynasore Improves Motor Function Recovery via Inhibition of Neuronal Apoptosis and Astrocytic Proliferation after Spinal Cord Injury in Rats. Mol Neurobiol. 2016 Nov 7. [Content Brief]
[5]. Liang Ma, et al. Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res. 2015 Jan;25(1):24-38. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1026 mL | 15.5130 mL | 31.0260 mL | 77.5651 mL |
| 5 mM | 0.6205 mL | 3.1026 mL | 6.2052 mL | 15.5130 mL | |
| 10 mM | 0.3103 mL | 1.5513 mL | 3.1026 mL | 7.7565 mL | |
| 15 mM | 0.2068 mL | 1.0342 mL | 2.0684 mL | 5.1710 mL | |
| 20 mM | 0.1551 mL | 0.7757 mL | 1.5513 mL | 3.8783 mL | |
| 25 mM | 0.1241 mL | 0.6205 mL | 1.2410 mL | 3.1026 mL | |
| 30 mM | 0.1034 mL | 0.5171 mL | 1.0342 mL | 2.5855 mL | |
| 40 mM | 0.0776 mL | 0.3878 mL | 0.7757 mL | 1.9391 mL | |
| 50 mM | 0.0621 mL | 0.3103 mL | 0.6205 mL | 1.5513 mL | |
| 60 mM | 0.0517 mL | 0.2586 mL | 0.5171 mL | 1.2928 mL | |
| 80 mM | 0.0388 mL | 0.1939 mL | 0.3878 mL | 0.9696 mL | |
| 100 mM | 0.0310 mL | 0.1551 mL | 0.3103 mL | 0.7757 mL |
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