Art. -Nr.: HY-B2098

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Lucanthone

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Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).

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Reinheit: 99.63%
CAS. Nr.: 479-50-5
Formel: C₂₀H₂₄N₂OS
Molecular Weight:340.48

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Lucanthone Chemical Structure

CAS. Nr.: 479-50-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).

IC₅₀ & Target

APE-1^[1]

Alle Parasite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
BT-20	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human BT20 cells after 72 hrs by MTT assay Cytotoxicity against human BT20 cells after 72 hrs by MTT assay	[PMID: 25699157]
BT-474	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human BT474 cells after 72 hrs by MTT assay Cytotoxicity against human BT474 cells after 72 hrs by MTT assay	[PMID: 25699157]
HCC1937	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human HCC1937 cells after 72 hrs by MTT assay Cytotoxicity against human HCC1937 cells after 72 hrs by MTT assay	[PMID: 25699157]
HCC1954	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human HCC1954 cells after 72 hrs by MTT assay Cytotoxicity against human HCC1954 cells after 72 hrs by MTT assay	[PMID: 25699157]
MDA-MB-231	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 25699157]
MDA-MB-435	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTT assay	[PMID: 25699157]
SK-BR-3	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 25699157]
Vero 76	IC₅₀	12.4 μM Compound: 19	Cytotoxicity against green monkey Vero 76 cells incubated for 48 to 96 hrs by crystal violet staining assay Cytotoxicity against green monkey Vero 76 cells incubated for 48 to 96 hrs by crystal violet staining assay	[PMID: 36753881]

In Vitro

Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. To investigate the anticancer activity of Lucanthone, cell viability is measured by MTT assay. Lucanthone reduces cell viability to a similar extent in a panel of seven breast cancer cell lines. In addition, a direct comparison reveals that Lucanthone is significantly more potent than Chloroquine (CQ) at reducing breast cancer cell viability with a mean IC₅₀ of 7.2 μM versus 66 μM for CQ. Measurement of cell viability in two representative cell lines (MDA-MB-231 and BT-20) by ATPlite assay and trypan blue exclusion reveals comparable results^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 479-50-5
Appearance Solid
Molecular Weight 340.48
Formel C₂₀H₂₄N₂OS
Color Off-white to orange
SMILES

O=C1C2=C(SC3=C1C=CC=C3)C(C)=CC=C2NCCN(CC)CC
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (73.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9370 mL

14.6852 mL

29.3703 mL

5 mM

0.5874 mL

2.9370 mL

5.8741 mL

10 mM

0.2937 mL

1.4685 mL

2.9370 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Protokoll

Cell Assay^[2]

Cell viability is assessed by MTT assay. Cells are seeded into 96-well microculture plates at 10,000 cells per well and allowed to attach for 24 h. Cells are then treated with Lucanthone (0, 0.5, 1, 5, 10, 20 and 40 μM), Chloroquine, Vorinostat, or combinations for 72 h. Following drug treatment, MTT is added and cell viability is quantified using a BioTek microplate reader. Effects on cell viability are also determined by measuring ATP levels using the ATPlite assay system and by trypan blue exclusion. Pro-apoptotic effects following in vitro drug exposure are quantified by propidium iodide (PI) staining and fluorescence-activated cell sorting (FACS) analysis of sub-G₀/G₁ DNA content^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Chowdhury SM, et al. Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme. Nanomedicine. 2015 Jan;11(1):109-18. [Content Brief]

[2]. Carew JS, et al. Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. J Biol Chem. 2011 Feb 25;286(8):6602-13. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9370 mL	14.6851 mL	29.3703 mL	73.4257 mL
	5 mM	0.5874 mL	2.9370 mL	5.8741 mL	14.6851 mL
	10 mM	0.2937 mL	1.4685 mL	2.9370 mL	7.3426 mL
	15 mM	0.1958 mL	0.9790 mL	1.9580 mL	4.8950 mL
	20 mM	0.1469 mL	0.7343 mL	1.4685 mL	3.6713 mL
	25 mM	0.1175 mL	0.5874 mL	1.1748 mL	2.9370 mL
	30 mM	0.0979 mL	0.4895 mL	0.9790 mL	2.4475 mL
	40 mM	0.0734 mL	0.3671 mL	0.7343 mL	1.8356 mL
	50 mM	0.0587 mL	0.2937 mL	0.5874 mL	1.4685 mL
	60 mM	0.0490 mL	0.2448 mL	0.4895 mL	1.2238 mL

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Lucanthone

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Alle Parasite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Lucanthone

Anti-Infection Compound Library

FDA-Approved Drug Library

Anti-Cancer Compound Library

Autophagy Compound Library

Drug Repurposing Compound Library

FDA Approved & Pharmacopeial Drug Library

Anti-Breast Cancer Compound Library

Antiparasitic Compound library

Targeted Diversity Library

FDA-Approved Anticancer Drug Library

Human Metabolite Library

Off-patent Drug Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

Anti-Brain Cancer Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Alle Parasite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Lucanthone Related Classifications

HY-B2098 Related Classifications