Art. -Nr.: HY-175324

MG-HuR2

Name: MG-HuR2
Brand: MedChemExpress
SKU: HY-175324
Price: 80.00 USD
Availability: InStock
Rating: 5 (1 reviews)

1 Cited Publications

Kundenbewertung

Based on 1 publication(s) in Google Scholar

MG-HuR2 is a molecular glue degrader targeting HuR (ELAVL1) with a K_d of 0.2018 μM. MG-HuR2 recruits the E3 ubiquitin ligase RNF126 and induces proteasome-dependent degradation of HuR. MG-HuR2 exhibits a unique biphasic degradation profile. MG-HuR2 reduces the expression of HuR and its downstream targets Bcl2/FOXQ1, inhibits breast cancer cell proliferation, enhances cytotoxicity and apoptosis (apoptosis), and suppresses the growth of 3D breast cancer tumor spheres. MG-HuR2 can be used in studies related to breast cancer and HuR-targeted protein degradation.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 98.70%
CAS. Nr.: 3098773-64-6
Formel: C₂₂H₂₀N₂O₅
Molecular Weight:392.40

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

MG-HuR2 Chemical Structure

CAS. Nr.: 3098773-64-6

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[1]

HuR

0.2018 μM (Kd)

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

In Vitro

MG-HuR2 binds tightly to purified HuR RRM1/2 protein in fluorescence polarization displacement assays, with a K_d value of 0.2018 μM^[1].
MG-HuR2 (10 μM; 24 h) effectively degrades GFP-tagged HuR in HEK-293 cells^[1].
MG-HuR2 binds to two distinct RNA-binding pockets on HuR. Calculations of binding energy via MM-GBSA show that its affinity for the P1 pocket (-46.76 kcal/mol) is higher than that for the P2 pocket (-39.04 kcal/mol)^[1].
MG-HuR2 (1 nM-10 μM; 24 h) induces proteasome-dependent biphasic degradation of HuR in MCF-7 and MDA-MB-231 breast cancer cells. After 24 h, HuR expression is reduced by up to 85% at 10 nM and up to 80% at 10 μM^[1].
MG-HuR2 (1-100 nM) reduces the expression of HuR-associated Bcl2 and FOXQ1 mRNA in MCF-7 cells; Bcl2 mRNA rebounds at 1 μM, which is consistent with the hook effect of MG-HuR2^[1].
MG-HuR2 (10 nM; 24 h) reduces Bcl2 protein expression in MCF-7 and MDA-MB-231 cells by 88% and 45%, respectively^[1].
MG-HuR2 (10 nM or 10 μM) reduces the viability of MCF-7 breast cancer cells, whereas it causes no significant cytotoxicity or change in cell viability in non-tumorigenic mammary epithelial MCF-10A cells^[1].
MG-HuR2 (0.1 nM-100 μM; 24 or 48 h) significantly inhibits the proliferation of MCF-7 and MDA-MB-231 breast cancer cells, with a more pronounced inhibitory effect at 48 h^[1].
MG-HuR2 (10 nM) inhibits the growth of tumorspheres starting from day 2 and continuously reduces the surface area of tumorspheres during the subsequent observation period in 3D breast cancer tumorsphere models of MCF-7 and MDA-MB-231^[1].
MG-HuR2 (1 nM-1 μM; 24 or 48 h) enhances caspase-3/7 activity in MCF-7 cells and significantly induces apoptosis at 10 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MCF-7 breast cancer cells, MDA-MB-231 breast cancer cells
Concentration:

10 nM-10 μM
Incubation Time:

24 h
Result:

Reduced HuR by 85% and 56% at 10 nM and by 80% and 59% at 10 μM in MCF-7 and MDA-MB-231 cells, respectively, with a biphasic degradation profile.

Western Blot Analysis^[1]

Cell Line:

MCF-7 breast cancer cells
Concentration:

MG-HuR2 10 μM; MG132 or bortezomib 1 μM
Incubation Time:

5 h
Result:

Proteasome inhibition blocked HuR degradation, supporting a proteasome-dependent degradation mechanism.

RT-PCR^[1]

Cell Line:

MCF-7 breast cancer cells
Concentration:

1 nM-1 μM
Incubation Time:

24 h
Result:

Reduced Bcl2 mRNA at low nanomolar concentrations, with rebound at 1 μM consistent with the hook effect.

Cell Proliferation Assay^[1]

Cell Line:

MCF-7 breast cancer cells, MDA-MB-231 breast cancer cells
Concentration:

0.1 nM-100 μM
Incubation Time:

24 h; 48 h
Result:

Significantly reduced cell proliferation, with stronger inhibition at 48 h.

Apoptosis Analysis^[1]

Cell Line:

MCF-7 breast cancer cells
Concentration:

1 nM-1 μM
Incubation Time:

24 h; 48 h
Result:

Increased caspase-3/7-dependent apoptosis, with a significant increase at 10 nM.

Chemical Information

CAS. Nr. 3098773-64-6
Appearance Solid
Molecular Weight 392.40
Formel C₂₂H₂₀N₂O₅
Color Light yellow to orange
SMILES

CCOC(C1=CC2=CC(NC(/C=C/C(C3=CC=C(C=C3)OC)=O)=O)=CC=C2N1)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

All

Sort by:

Journal Impact Factor

Res Sq

2026 Jun 15.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (254.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5484 mL

12.7421 mL

25.4842 mL

5 mM

0.5097 mL

2.5484 mL

5.0968 mL

10 mM

0.2548 mL

1.2742 mL

2.5484 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (12.74 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kassabri L, et al. Druglike Molecular Degraders of the Oncogenic RNA-Binding Protein HuR. JACS Au. 2025 Aug 25;5(8):3879-3891. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5484 mL	12.7421 mL	25.4842 mL	63.7105 mL
	5 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	10 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	15 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	20 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	25 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	30 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	40 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	50 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL
	60 mM	0.0425 mL	0.2124 mL	0.4247 mL	1.0618 mL
	80 mM	0.0319 mL	0.1593 mL	0.3186 mL	0.7964 mL
	100 mM	0.0255 mL	0.1274 mL	0.2548 mL	0.6371 mL

Bioz

Keywords

MG-HuR2

1 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

MG-HuR2

Publications Citing Use of MedChemExpress (MCE) MG-HuR2

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen