Art. -Nr.: HY-N4107

Phyllanthin

1 Cited Publications

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Phyllanthin is an effective oral anticancer agent. Phyllanthin inhibits MOLT-4 cell viability, increases apoptosis, inhibits cell migration and invasion. Phyllanthin exerts anti-fibrotic effects by down-regulating TGF signaling pathway via ALK5 and Smad2/3. Phyllanthin also has anti-inflammatory and antibacterial properties .

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Reinheit: 99.97%
CAS. Nr.: 10351-88-9
Formel: C₂₄H₃₄O₆
Molecular Weight:418.52

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Phyllanthin Chemical Structure

CAS. Nr.: 10351-88-9

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Inhibits TGF-β1 with inhibition constant of 33.15 mM.

Alle TGF-beta/Smad Isoform-spezifische Produkte anzeigen :

Smad1
Smad3

In Vitro

Phyllanthin (1.56, 6.25 and 25μM; 2 h) inhibits LPS (HY-D1056) induced proinflammatory responses in U937 macrophages ^[4].
Phyllanthin (5-100 μM/mL; 24 h) inhibits cell growth with an IC ₅₀ value of 25 µM/mL, and induces apoptosis in MOLT-4 leukemia cancer cells ^[3].
Phyllanthin is predicted to have strong activity against COVID-19 through inhibiting spike glycoprotein and main protease ^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3] ^[4]

Cell Line:

U937 macrophages, MOLT-4 cells and normal MS‐5 cells
Concentration:

1.56, 3.12, 6.25, 12.5, and 25 μM (U937 macrophages);
5, 10, 25, 50, 75, and 100 μM/mL (MOLT-4 cells and normal MS‐5 cells)
Incubation Time:

24 h
Result:

No effect on the viability of U937 macrophages and MS‐5 cells.
Reduced cell viability of MOLT-4 in a dose-dependent manner.

Western Blot Analysis^[3] ^[4]

Cell Line:

LPS (1 μg/mL) (HY- D1056) treated U937 macrophages for 30 min
Concentration:

1.56, 6.25, and 25 μM
Incubation Time:

2 h
Result:

Inhibited phosphorylation levels of IKKα/β, p65 and IκBα in a concentration‐dependent manner.
Suppressed LPS‐stimulated activation of JNK, ERK, p38 and AKT in a concentration‐dependent manner.

Real Time qPCR^[4]

Cell Line:

LPS (1 μg/mL) (HY- D1056) treated U937 macrophages for 1 day
Concentration:

1.56, 6.25 and 25 μM
Incubation Time:

2 h
Result:

Dose-dependently suppressed the increased TNF‐α mRNA level at concentrations of 1.25, 6.25 and 25 μM.
Significantly suppressed IL‐1β gene expression at concentrations of 6.25 and 25 μM.

In Vivo

Phyllanthin (10 mg/kg; oral administration; once a day; 30 days) has protective effects in carbon tetrachloride (HY-Y0298) induced hepatotoxicity and experimental liver fibrosis in mice ^[2].
Phyllanthin (30 mg/kg; oral administration; single dose) exerts anti-tumor effects in diethylnitrosamine (HY-N7434) induced liver carcinogenesis in rats ^[5].
Phyllanthin (20-100 mg/kg; oral administration; once a day; 14 days) inhibits cellular and humoral immune responses in Balb/C mice ^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Carbon tetrachloride (HY-Y0298) treated Swiss strain female mice (32-35 g) ^[2]
Dosage:

10 mg/kg
Administration:

Oral administration (p.o.); once a day; 30 days
Result:

Normalized the level of liver marker enzymes (ALT and AST) and collagen content in hepatotoxicity and experimental liver fibrosis mice.
Restored normal liver architecture, reversed the formation of continuous fibrotic septa between the central and portal veins and nodules.
Reduced the levels of TGF-β1, ALK5, p-Smad2 and p-Smad3 in hepatotoxicity and experimental liver fibrosis mice.

Animal Model:

Diethylnitrosamine (Cat. No. HY-N7434) treated male Wistar albino rats (210-230 g)^[5]
Dosage:

30 mg/kg
Administration:

Oral administration (p.o.); single dose
Result:

Significantly reduced 8-OHdG levels and delayed the oxidative damage in diethylnitrosamine -induced liver cancer rats.
Significantly reduced liver tissue injury biomarkers (ALT, AST, GGT, ALP and LDH) levels and tumor markers (AFP and CEA).
Reduced cellular enlargement, nodules, and hyperplasiaenlargement in diethylnitrosamine-induced liver tissue.

Animal Model:

Male Balb/C mice aged 6-8 weeks old (18-22 g) ^[6]
Dosage:

20 mg/kg, 40 mg/kg, and 100 mg/kg
Administration:

Oral administration (p.o.); once a day; 14 days
Result:

Dose-dependently inhibited CD11b/CD18 adhesion, the engulfment of E.coli by peritoneal macrophages molecules, NO and MPO release.
Dose-dependently inhibited T and B lymphocytes proliferation and down-regulated Th1 (IL-2 and IFN-γ) and Th2 (IL-4) cytokines.
Dose-dependently inhibited the sheep red blood cell (sRBC)-induced swelling rate of mice paw in delayed type hypersensitivity.
Significantly inhibited the serum levels of ceruloplasmin and lysozyme at higher doses (40 and 100 mg/kg).
Dose-dependently down-regulated anti-sRBC immunoglobulins (IgM and IgG) antibody titer.

Chemical Information

CAS. Nr. 10351-88-9
Appearance Solid
Molecular Weight 418.52
Formel C₂₄H₃₄O₆
Color White to off-white
SMILES

COC[C@@H](CC1=CC=C(OC)C(OC)=C1)[C@@H](COC)CC2=CC=C(OC)C(OC)=C2
Structure Classification
- Phenylpropanoids
- Lignans
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

University of Edinburgh

2025 May 29.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 62.5 mg/mL (149.34 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3894 mL

11.9469 mL

23.8937 mL

5 mM

0.4779 mL

2.3894 mL

4.7787 mL

10 mM

0.2389 mL

1.1947 mL

2.3894 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Hassanein EHM, et al. The promising antioxidant effects of lignans: Nrf2 activation comes into view. Naunyn Schmiedebergs Arch Pharmacol. 2024 Sep;397(9):6439-6458. [Content Brief]

[2]. Krithika R, et al. Mechanism of protective effect of phyllanthin against carbon tetrachloride-induced hepatotoxicity and experimental liver fibrosis in mice. Toxicol Mech Methods. 2015;25(9):708-17. [Content Brief]

[3]. Wang H, et al. Phyllanthin inhibits MOLT-4 leukemic cancer cell growth and induces apoptosis through the inhibition of AKT and JNK signaling pathway. J Biochem Mol Toxicol. 2021 Jun;35(6):1-10. [Content Brief]

[4]. Harikrishnan H, et al. Phyllanthin from Phyllanthus amarus inhibits LPS-induced proinflammatory responses in U937 macrophages via downregulation of NF-κB/MAPK/PI3K-Akt signaling pathways. Phytother Res. 2018 Dec;32(12):2510-2519. [Content Brief]

[5]. You Y, et al. Phyllanthin prevents diethylnitrosamine (DEN) induced liver carcinogenesis in rats and induces apoptotic cell death in HepG2 cells. Biomed Pharmacother. 2021 May;137:111335. [Content Brief]

[6]. Ilangkovan M, et al. Phyllanthin from Phyllanthus amarus inhibits cellular and humoral immune responses in Balb/C mice. Phytomedicine. 2016 Nov 15;23(12):1441-1450. [Content Brief]

[7]. Marhaeny HD, et al. Phyllanthin and hypophyllanthin, the isolated compounds of Phyllanthus niruri inhibit protein receptor of corona virus (COVID-19) through in silico approach. J Basic Clin Physiol Pharmacol. 2021 Jun 25;32(4):809-815. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3894 mL	11.9469 mL	23.8937 mL	59.7343 mL
	5 mM	0.4779 mL	2.3894 mL	4.7787 mL	11.9469 mL
	10 mM	0.2389 mL	1.1947 mL	2.3894 mL	5.9734 mL
	15 mM	0.1593 mL	0.7965 mL	1.5929 mL	3.9823 mL
	20 mM	0.1195 mL	0.5973 mL	1.1947 mL	2.9867 mL
	25 mM	0.0956 mL	0.4779 mL	0.9557 mL	2.3894 mL
	30 mM	0.0796 mL	0.3982 mL	0.7965 mL	1.9911 mL
	40 mM	0.0597 mL	0.2987 mL	0.5973 mL	1.4934 mL
	50 mM	0.0478 mL	0.2389 mL	0.4779 mL	1.1947 mL
	60 mM	0.0398 mL	0.1991 mL	0.3982 mL	0.9956 mL
	80 mM	0.0299 mL	0.1493 mL	0.2987 mL	0.7467 mL
	100 mM	0.0239 mL	0.1195 mL	0.2389 mL	0.5973 mL

Keywords

Phyllanthin

1 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Phyllanthin

Alle TGF-beta/Smad Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Phyllanthin Related Classifications

HY-N4107 Related Classifications