Spadin
Based on 1 publication(s) in Google Scholar
Spadin, a natural peptide derived from a propeptide released in blood, is a potent TREK-1 channel blocker with IC50 value of 10 nM. Spadin enhances dorsal raphe nucleus 5-HT neurotransmission in mice and induces hippocampal CREB activation and neurogenesis. Spadin can be used for antidepressant research.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.84%
- CAS. Nr.: 1270083-24-3
- Formel: C96H142N26O22
- Molecular Weight:2012.34
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Speicherung:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Spadin
MoreAlle 5-HT Receptor Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Spadin (100 nM; COS-7 cells) has inhibitory effect of spadin on the TREK-1 channel and blocks 63% of the TREK-1 current stimulated by arachidonic acid[1].
Spadin (100 nM) blocks the TREK-1 channels activity in CA3 hippocampal neurons on brain slices of wild-type mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Spadin (0.01-100 μM; ICV, i.p. and i.v.; daily, for 7 days; male C57Bl/6J and TREK-1 deficient mice) has anti-depressant behavior in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57Bl/6J and TREK-1 deficient mice[2]
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Dosage:10 μM
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Administration:Intraperitoneal injection; for 30 min
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Result:Increased of the 5-HT neuron firing rate in the dorsal raphe nucleus (DRN).
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Animal Model:Male C57Bl/6J and TREK-1 deficient mice[2]
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Dosage:0.01-100 μM
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Administration:Intracerebroventrical injection, intraperitoneal injection and intravenous injection; daily, for 7 days
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Result:Had any effect on mouse locomotion analyzed in short- or long-time after the drug injection.
Chemical Information
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CAS. Nr. 1270083-24-3
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Appearance Solid
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Molecular Weight 2012.34
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Formel C96H142N26O22
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Color White to off-white
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Sequence Shortening
YAPLPRWSGPIGVSWGLR
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Biol Chem
Validation of TREK1 ion channel activators as an immunomodulatory and neuroprotective strategy in neuroinflammation. [Abstract]2023 Feb 14;404(4):355-375. PMID: 36774650
Lösungsmittel & Löslichkeit
H2O : ≥ 100 mg/mL (49.69 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Borsotto M, et, al. Targeting two-pore domain K(+) channels TREK-1 and TASK-3 for the treatment of depression: a new therapeutic concept. Br J Pharmacol. 2015 Feb;172(3):771-84. [Content Brief]
[2]. Mazella J, et, al. Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design. PLoS Biol. 2010 Apr 13;8(4):e1000355. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4969 mL | 2.4847 mL | 4.9693 mL | 12.4233 mL |
| 5 mM | 0.0994 mL | 0.4969 mL | 0.9939 mL | 2.4847 mL | |
| 10 mM | 0.0497 mL | 0.2485 mL | 0.4969 mL | 1.2423 mL | |
| 15 mM | 0.0331 mL | 0.1656 mL | 0.3313 mL | 0.8282 mL | |
| 20 mM | 0.0248 mL | 0.1242 mL | 0.2485 mL | 0.6212 mL | |
| 25 mM | 0.0199 mL | 0.0994 mL | 0.1988 mL | 0.4969 mL | |
| 30 mM | 0.0166 mL | 0.0828 mL | 0.1656 mL | 0.4141 mL | |
| 40 mM | 0.0124 mL | 0.0621 mL | 0.1242 mL | 0.3106 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Spadin Related Classifications
HY-P1422 Related Classifications
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