Art. -Nr.: HY-149007

STAT3-IN-11

Name: STAT3-IN-11
Brand: MedChemExpress
SKU: HY-149007
Price: 270.00 USD
Availability: InStock
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STAT3-IN-11 (7a) is a selective STAT3 inhibitor that inhibits the phosphorylation of STAT3 at site pTyr705. STAT3-IN-11 inhibits the phosphorylation of downstream genes (Survivin and Mcl-1) without affecting its upstream tyrosine kinases (Src and JAK2) levels and p-STAT1 expression. STAT3-IN-11 can induce cancer cell apoptosis, which is potential for the discovery of effective STAT3 inhibitors and antitumor agents against cancers.

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Reinheit: 98.29%
CAS. Nr.: 2503096-50-0
Formel: C₂₀H₁₇NO₄
Molecular Weight:335.35

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

STAT3-IN-11 Chemical Structure

CAS. Nr.: 2503096-50-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC₅₀: 1.93 μM (SIP), ≥ 30 μM (SphK1), ≈ 30 μM (SphK2)^[1].

Alle STAT Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	7.02 μM Compound: 147	Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay	[PMID: 38458106]
HepG2	IC₅₀	7.2 μM Compound: 147	Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay	[PMID: 38458106]
MDA-MB-231	IC₅₀	6.01 μM Compound: 147	Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay	[PMID: 38458106]

In Vitro

STAT3-IN-11 (20 μM, 48 h) has 97.86% inhibitory effect on MDA-MB-231 cells^[1].
STAT3-IN-11 (0-30 μM, 48 h) inhibits several cancer cells with IC₅₀ values of 6.01 μM (MDA-MB-231), 7.02μM (HepG2, A549), and normal human cells with IC₅₀ values of 26.54 μM (MDA-MB-10A), 26.69 μM (PBMCs), 12.52 μM (HFL-1) ^[1].
STAT3-IN-11 (2.5-10 μM, 6 h) inhibits the phosphorylation of STAT3 (stimulated by IL-6 in MDA-MB-231) at site pTyr705 in a dose-dependent manner^[1].
STAT3-IN-11 (2.5-10 μM, 6 h) inhibits the expression of the downstream gene (Survivin and Mcl-1) of STAT3 in a concentration-dependent manner^[1].
STAT3-IN-11 (2.5-10 μM, 6 h) has no effects on the typical upstream kinases of STAT3 such as p-JAK2 and p-Src and the phosphorylation of STAT1 (a STAT isoform) ^[1].
STAT3-IN-11 (2.5-10 μM, 48 h) can induce cell apoptosis in a dose-manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

20 μM
Incubation Time:

48 hours
Result:

The antiproliferative activity reached 97.86%.

Cell Cytotoxicity Assay^[1]

Cell Line:

MDA-MB231, A549, MDA-MB-10A, PBMCs and HFL-1 cells
Concentration:

20 μM
Incubation Time:

48 hours
Result:

Inhibited with IC₅₀ values of 6.01, 7.20, 7.02, 26.54, 25.69 and 12.52 μM for MDA-MB231, A549, MDA-MB-10A, PBMCs and HFL-1 cells, respectively.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

2.5,5 and 10 μM
Incubation Time:

6 hours
Result:

Decrease the level of p-STAT3 at site pTyr705 in a concentration dependent manner.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

2.5,5 and 10 μM
Incubation Time:

6 hours
Result:

Down-regulated the level of STAT3 downstream genes.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

5, 10 and 15 μM
Incubation Time:

48 hours
Result:

The percentage of apoptotic cells was 24.4% at a concentration of 15 μM.

Chemical Information

CAS. Nr. 2503096-50-0
Appearance Solid
Molecular Weight 335.35
Formel C₂₀H₁₇NO₄
Color Yellow to orange
SMILES

O=C(NCCC(C1=O)=C(C)C(C2=C1C(O)=CC=C2)=O)C3=CC=CC=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

J Nutr Biochem

Calorie restriction mimetic, resveratrol, attenuates hepatic ischemia and reperfusion injury through enhancing efferocytosis of macrophages via AMPK/STAT3/S1PR1 pathway. [Abstract]

2024 Apr:126:109587. PMID: 38262562

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (298.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9820 mL

14.9098 mL

29.8196 mL

5 mM

0.5964 mL

2.9820 mL

5.9639 mL

10 mM

0.2982 mL

1.4910 mL

2.9820 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (7.45 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Li N, et al. Design, synthesis and biological evaluation of novel plumbagin derivatives as potent antitumor agents with STAT3 inhibition. Bioorg Chem. 2020 Nov;104:104208. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9820 mL	14.9098 mL	29.8196 mL	74.5490 mL
	5 mM	0.5964 mL	2.9820 mL	5.9639 mL	14.9098 mL
	10 mM	0.2982 mL	1.4910 mL	2.9820 mL	7.4549 mL
	15 mM	0.1988 mL	0.9940 mL	1.9880 mL	4.9699 mL
	20 mM	0.1491 mL	0.7455 mL	1.4910 mL	3.7274 mL
	25 mM	0.1193 mL	0.5964 mL	1.1928 mL	2.9820 mL
	30 mM	0.0994 mL	0.4970 mL	0.9940 mL	2.4850 mL
	40 mM	0.0745 mL	0.3727 mL	0.7455 mL	1.8637 mL
	50 mM	0.0596 mL	0.2982 mL	0.5964 mL	1.4910 mL
	60 mM	0.0497 mL	0.2485 mL	0.4970 mL	1.2425 mL
	80 mM	0.0373 mL	0.1864 mL	0.3727 mL	0.9319 mL
	100 mM	0.0298 mL	0.1491 mL	0.2982 mL	0.7455 mL

Bioz

Keywords

STAT3-IN-11

1 Cited Publications

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STAT3-IN-11

Publications Citing Use of MedChemExpress (MCE) STAT3-IN-11

Alle STAT Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

STAT3-IN-11 Related Classifications

HY-149007 Related Classifications

Bioz