BIBO3304
Based on 4 publication(s) in Google Scholar
BIBO3304, a chemical probe, is a potent, orally active, and selective neuropeptide Y (NPY) Y1 receptor antagonist, with subnanomolar affinity for both the human and the rat Y1 receptor (IC50=0.38 and 0.72 nM, respectively).
For research use only. We do not sell to patients.
The BIBO3304 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.
- Purity: 99.96%
- CAS No.: 191868-14-1
- Formula: C31H36F3N7O5
- Molecular Weight:643.66
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) BIBO3304
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Others
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In Vivo Efficacy Study
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Others
All Neuropeptide Y Receptor Isoforms
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Biological Activity
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NPY Y1 receptor |
BIBO3304 (15-60 μg) dose-dependently inhibits the feeding reponse mediated by 1 μg NPY[1].
BIBO3304 (0.5 μM; p.o.) significantly increases serum insulin levels[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult male Chbb:Thom rats weighing between 300 and 340 g[1]
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Dosage:30 μg
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Administration:bilateral paraventricular nucleus injection
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Result:Attenuated the hyperphagia following fasting, especially during the first 2 h of refeeding.
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Animal Model:7-week-old C57BL/6JAusb mice[2]
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Dosage:0.5 μM
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Administration:p.o.
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Result:Significantly increased serum insulin levels.
Chemical Information
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CAS No. 191868-14-1
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Appearance Solid
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Molecular Weight 643.66
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Formula C31H36F3N7O5
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Color White to off-white
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SMILES
O=C(N[C@@H](C(NCC1=CC=C(CNC(N)=O)C=C1)=O)CCCNC(N)=N)C(C2=CC=CC=C2)C3=CC=CC=C3.O=C(O)C(F)(F)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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Nat Commun
Neuropeptide Y neurons mediate opioid-induced itch by disinhibiting GRP-GRPR microcircuits in the spinal cord. [Abstract]2025 Aug 1;16(1):7074. PMID: 40750771
BIBO3304 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 1;16(1):7074. [Abstract]
Intrathecal injection of NPY1R selective antagonist BIBO3304 (BIBO, 10 μg, 20 min before) blocked the effects of LP-NPY (3 μg) on morphine-induced itch, one-way ANOVA, Dunnett’s multiple comparisons.
BIBO3304 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 1;16(1):7074. [Abstract]
NPY (10 μM) induced outward currents were recorded and inhibited by BIBO3304(2 μM) from GRP+ interneurons in the SDH from GrpCre;Ai9 mice.
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Nat Commun
2024 Sep 1;15(1):7603. PMID: 39217143 -
Brain
2026 Feb 25:awag077. PMID: 41738433 -
In Vivo
Ghrelin Induces the Production of Hypothalamic NPY Through the AMPK-mTOR Pathway to Alleviate Cancer-induced Bone Pain. [Abstract]2024 May-Jun;38(3):1133-1142. PMID: 38688635
BIBO3304 purchased from MedChemExpress. Usage Cited in: In Vivo. 2024 May-Jun;38(3):1133-1142. [Abstract]
Hypothalamic NPY exerted an antinociceptive effect by binding to Y1R and Y2R in cancer-bearing rats. PWT was measured using von Frey filaments (BIBO3304: 3 μg).
Solvent & Solubility
DMSO : 100 mg/mL (155.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wieland HA, et al. Subtype selectivity of the novel nonpeptide neuropeptide Y Y1 receptor antagonist BIBO 3304 and its effect on feeding in rodents. Br J Pharmacol. 1998 Oct;125(3):549-55. [Content Brief]
[2]. Loh K, et al. Inhibition of Y1 receptor signaling improves islet transplant outcome. Nat Commun. 2017 Sep 8;8(1):490. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5536 mL | 7.7681 mL | 15.5362 mL | 38.8404 mL |
| 5 mM | 0.3107 mL | 1.5536 mL | 3.1072 mL | 7.7681 mL | |
| 10 mM | 0.1554 mL | 0.7768 mL | 1.5536 mL | 3.8840 mL | |
| 15 mM | 0.1036 mL | 0.5179 mL | 1.0357 mL | 2.5894 mL | |
| 20 mM | 0.0777 mL | 0.3884 mL | 0.7768 mL | 1.9420 mL | |
| 25 mM | 0.0621 mL | 0.3107 mL | 0.6214 mL | 1.5536 mL | |
| 30 mM | 0.0518 mL | 0.2589 mL | 0.5179 mL | 1.2947 mL | |
| 40 mM | 0.0388 mL | 0.1942 mL | 0.3884 mL | 0.9710 mL | |
| 50 mM | 0.0311 mL | 0.1554 mL | 0.3107 mL | 0.7768 mL | |
| 60 mM | 0.0259 mL | 0.1295 mL | 0.2589 mL | 0.6473 mL | |
| 80 mM | 0.0194 mL | 0.0971 mL | 0.1942 mL | 0.4855 mL | |
| 100 mM | 0.0155 mL | 0.0777 mL | 0.1554 mL | 0.3884 mL |
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