Cat. No.: HY-12238

IWR-1

(Synonyms: endo-IWR 1; IWR-1-endo)

67 Cited Publications

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Based on 67 publication(s) in Google Scholar

IWR-1 (IWR-1-endo) is a tankyrase inhibitor targeting Wnt/β-catenin (IC₅₀ = 180 nM). IWR-1 compromises critical steps of the canonical Wnt signaling, namely translocation of β-catenin to the nucleus and subsequent TCF/LEF activation and expression of Wnt/β-catenin downstream targets. IWR-1 promotes β-catenin phosphorylation by promoting stability of Axin-scaffolded destruction complexes. IWR-1 can be studied in research for anti-tumor purposes, and diseases such as osteosarcoma, colorectal cancer and psoriasis.

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Pureté: 99.60%
CAS No.: 1127442-82-3
Formule: C₂₅H₁₉N₃O₃
Masse moléculaire:409.44

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

IWR-1 Chemical Structure

CAS No.: 1127442-82-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (67)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

IC50: 180 nM (Wnt)

Voir tous les produits spécifiques à Isoform Wnt :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	>100 μM Compound: IWR1	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 24950489]
HEK293	IC₅₀	136 μM Compound: 1, IWR1	Inhibition of Tankyrase in human HEK293 cells assessed as inhibition of Wnt pathway by Wnt3a-induced STF assay Inhibition of Tankyrase in human HEK293 cells assessed as inhibition of Wnt pathway by Wnt3a-induced STF assay	[PMID: 23316926]
HEK-293T	IC₅₀	26 nM Compound: 1, IWR-1	Inhibition of beta-casein-dependent canonical Wnt3 pathway in human HEK293T cells by luciferase reporter gene assay Inhibition of beta-casein-dependent canonical Wnt3 pathway in human HEK293T cells by luciferase reporter gene assay	[PMID: 22191557]
HepG2	GI₅₀	95.4 μM Compound: IWR1	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 24950489]
HT-29	GI₅₀	>100 μM Compound: IWR1	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 24950489]
HT-29	IC₅₀	24.4 μM Compound: IWR1	Inhibition of Wnt signaling in human HT29 cells assessed as inhibition of beta-catenin-mediated Tcf/Lef transcriptional activity after 24 hrs by dual luciferase reporter gene assay relative to control Inhibition of Wnt signaling in human HT29 cells assessed as inhibition of beta-catenin-mediated Tcf/Lef transcriptional activity after 24 hrs by dual luciferase reporter gene assay relative to control	[PMID: 24950489]
HT-29	IC₅₀	9.52 μM Compound: IWR-1	Cytotoxicity against human HT-29 cells assessed as cell viability at 1.563 to 50 uM measured after 72 hrs by plate reader method Cytotoxicity against human HT-29 cells assessed as cell viability at 1.563 to 50 uM measured after 72 hrs by plate reader method	[PMID: 34062253]
LoVo	GI₅₀	63.1 μM Compound: IWR1	Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay	[PMID: 24950489]
SW480	EC₅₀	2.5 μM Compound: 1, IWR1	Inhibition of tankyrase in human SW480 cells assessed as accumulation of axin2 after 24 hrs by Hoechst dye-based method Inhibition of tankyrase in human SW480 cells assessed as accumulation of axin2 after 24 hrs by Hoechst dye-based method	[PMID: 23701517]
SW480	IC₅₀	0.25 μM Compound: 1, IWR1	Inhibition of tankyrase in human SW480 cells assessed as degradation of beta catenin after 40 to 48 hrs Inhibition of tankyrase in human SW480 cells assessed as degradation of beta catenin after 40 to 48 hrs	[PMID: 23701517]

In Vitro

IWR-1 is cytotoxic for osteosarcoma cancer stem-like cells (CSCs)^[1].
IWR-1 suppresses cell migration, invasion, and matrix metalloproteinase activities of colorectal cancer cell lines^[2].
IWR-1 (2.5-10 μM, 48-96 h) is effective in reducing spheres’ viability in a concentration- and time- dependent manner in parental and spheres from MG-63 and MNNG-HOS cell lines^[1].
IWR-1 (10 μM, 96 h) increases the number of TUNEL-positive cells, reaching 4.65- and 15.83-fold differences relative to control at 96 h and promoted the activation of caspases 3/7 reaching 2.15- and 1.27-fold in MG-63 and MNNG-HOS spheres^[1].
IWR-1 (10 μM, 48 h) induces a cell cycle arrest in the G2/M phase in spheres derived from MG-63 and MNNG-HOS cell lines and increases the percentage of cells in the S phase slightly^[1].
IWR-1 (10 μM, 48 h) inhibits secondary sphere-forming efficacy by approximately 53% and 55% of the first-generation 7-day old spheres in MG-6t4 and MNNG-HOS cells^[1].
IWR-1 (5-50 μM, 24-48 h) decreases the proliferation of HCT116 cells in a dose- and time-dependent manner^[2].
IWR-1 (5-50 μM, 24-48 h) inhibits TNF-α-stimulated migration in HCT116 and HT29 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MG-63 and MNNG-HOS spheres and parent cells
Concentration:

10 μM
Incubation Time:

96 h
Result:

Led to an upregulation of Bak and a downregulation of Bcl-2 (key proteins involved in mitochondrial-dependent apoptosis), which contributes to a Bak/Bcl-2 ratio >1 that prone the cells to undergo apoptosis.
Resulted in a higher β-catenin nuclear/cytoplasmic ratio in spheres, indicating an increased Wnt/β-catenin pathway activation in osteosarcoma spheres.
Stabilized Axin2 protein levels.
Diminished the protein expression levels of Cyclin D1 in both parental cells and spheres.

RT-PCR^[1]

Cell Line:

MG-63 and MNNG-HOS spheres and parent cells
Concentration:

10 μM
Incubation Time:

96 h
Result:

Led to an upregulation of Bak and a downregulation of Bcl-2 (key proteins involved in mitochondrial-dependent apoptosis), which contributes to a Bak/Bcl-2 ratio >1 that prone the cells to undergo apoptosis.
Results in a higher β-catenin nuclear/cytoplasmic ratio in spheres, indicating an increased Wnt/β-catenin pathway activation in osteosarcoma spheres.
Stabilized Axin2 protein levels.
Diminished the protein expression levels of Cyclin D1 in both parental cells and spheres.

Cell Viability Assay^[1]

Cell Line:

MG-63 and MNNG-HOS spheres and parent cells
Concentration:

2.5, 5, 7.5, 10 μM
Incubation Time:

48 and 96 h
Result:

Reduced cell viability when concentration is higher than 5 μM.
Elicited more than 70% reduction of cell viability in spheres derived from the two cell lines at 96 h with 10 μM.
Had minimal effect on parental cells since the Wnt/β-catenin signaling is absent in these cells.
Increased the susceptibility of spheres towards Doxorubicin (HY-15142) when treated in combination with increasing concentrations of Doxorubicin (0.01-100 μM).

Western Blot Analysis^[2]

Cell Line:

HCT116 cells
Concentration:

5, 10, 20, 50 μM
Incubation Time:

0, 4, 8, 24, 48 h
Result:

Increased the levels of the epithelial marker E-cadherin whilst decreased the mesenchymal markers N-cadherin, Vimentin, and Snail dose- and time-dependently.
Inhibited the EMT process in HCT116 cells effectively.
Decreased β-catenin expression and inhibited the EMT-like expressional changes whereby decreasing N-cadherin and Snail and increasing E-cadherin expressions, even in the presence of TNF-α (HY-P1860) (10 ng/mL for 24 h)-induced EMT stimulation.
Decreased the phosphorylation of Akt in a concentration- and time-dependent manner.
Decreased the surviving expression in a concentration- and time-dependent manner, thereby promoting tumor proliferation directly or indirectly through regulating cancer cell homeostasis.
Reduced MMP activities only when surviving was suppressed.

Real Time qPCR^[2]

Cell Line:

HCT116 cells
Concentration:

5, 10, 20, 50 μM
Incubation Time:

0, 4, 8, 24, 48 h
Result:

Inhibited EMT in the mRNA levels under the TNF-α-induced EMT stimulation.

In Vivo

IWR-1 (5 mg/kg, intratumorally, each 2 d for 12 d) induces a marked inhibition of tumor growth in osteosarcoma mouse model^[1].
IWR-1 (10 mg/kg, s.c., on days 1, 3, 5) ameliorates IL-36γ (2 μg/mouse on days 1, 3, 5)-mediated exacerbation of psoriatic skin lesions in an Imiquimod (IMQ) (HY-B0180)-induced psoriasis-like mice model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Immunocompromised nude mice (6-week old female Swiss nude) were injected sub-cutaneously the pGL4-transfected MNNG-HOS cells (2×10⁶ cells/100 mL PBS)^[1]
Dosage:

5 mg/kg
Administration:

Intratumorally, each 2 d for 12 d
Result:

Resulted in slower tumor growth rate and reduction in tumor size by 73% and 71% in comparison to control and to Doxorubicin-treated (8 mg/kg, i.p., each 4 d for 12 d) groups.
Enhanced the therapeutic efficacy of Doxorubicin shown by a greater reduction of tumor burden at the end of the treatment in opposite to Doxorubicin alone.

Animal Model:

Balb/c mice (aged 6-8 weeks) with shaven back and treated with a daily topical dose of IMQ cream^[3]
Dosage:

10 mg/kg
Administration:

Subcutaneous injection (s.c.) on days 1, 3, 5
Result:

Increased epidermal thickening with keratinocyte thickness.
Significantly diminished the effect of IMQ on hyperplasia in the IMQ+IWR-1 group.
Ameliorated the pathological changes in IL-36γ-induced psoriasiform skin lesion.
Reversed IL-36γ-mediated upregulation of inflammatory factors (IL-17 A and IFN-γ) in psoriatic lesions.
Reversed IL-36γ-mediated upregulation of β-catenin and DKK1 expression.

Chemical Information

CAS No. 1127442-82-3
Appearance Solid
Masse moléculaire 409.44
Formule C₂₅H₁₉N₃O₃
Color Off-white to yellow
SMILES

O=C(NC1=C2N=CC=CC2=CC=C1)C3=CC=C(N(C([C@]4([H])[C@](C5)([H])C=C[C@]5([H])[C@]64[H])=O)C6=O)C=C3
Synonyms

endo-IWR 1; IWR-1-endo
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (67)

All RT-PCR2D/3D Cell Culture and DifferentiationWBIF

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Journal Impact Factor

Cell Stem Cell

An inducible model of human post-implantation development derived from primed and naive stem cells. [Abstract]

2025 Oct 2;32(10):1509-1527.e9. PMID: 40885193
Cell Stem Cell

Modeling early gastrulation in human blastoids with DNA methylation patterns of natural blastocysts. [Abstract]

2025 Mar 6;32(3):409-425.e8. PMID: 39814012
Adv Funct Mater

Metabolically Glycoengineered Neural Stem Cells Boost Neural Repair After Cardiac Arrest. [Abstract]

2024 Apr 25;34(17):2309866. PMID: 39071865
Nat Commun

Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice. [Abstract]

2024 May 23;15(1):4393. PMID: 38782937

IWR-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 May 23;15(1):4393. [Abstract]

mRNA levels of lipid absorption markers of MODE-K cells treated with the Wnt inhibitor IWR-1 (10 μM).

IWR-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 May 23;15(1):4393. [Abstract]

Organoids derived from WT mice were incubated with 10 μM IWR-1 and 4 mM VPA or DMSO as a negative control for 1 day, n=3.
Cell Death Differ

The E3 ubiquitin ligase Itch plays an essential role in kidney fibrosis by inhibiting Dvl2/GSK3β/β-catenin signaling pathway. [Abstract]

2026 May 22. PMID: 42168361
Adv Sci (Weinh)

PDZK1-ULK1 Axis Triggers Lipophagy to Inhibit Tumor Progression and Sunitinib Resistance in Clear Cell Renal Cell Carcinoma. [Abstract]

2026 Apr;13(23):e11606. PMID: 41698049
Adv Sci (Weinh)

The Pathogenic Roles of Local Vitamin D Metabolism Defect in Valve Inflammation and Calcification. [Abstract]

2025 Oct 13:e01250. PMID: 41082345

IWR-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 13:e01250. [Abstract]

Blockade of β-catenin activation with IWR-1 (20 μM; preincubated FOR 2 h) did not affect Pi-induced VIC osteogenic differentiation, which was verified by staining for Alizarin red (E) and qPCR analysis of osteoblast-specific genes.
Adv Sci (Weinh)

Large-Scale Bioprinting of Human Epiblast-Like Models Featuring Disc-Shaped Morphogenesis and Gastrulation Events. [Abstract]

2025 Jun 5:e05340. PMID: 40470686
J Adv Res

Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis. [Abstract]

2025 Jul 7:S2090-1232(25)00506-5. PMID: 40633837
Nano Today

21 September 2022.
MedComm (2020)

Gene Therapy Targeting Pkp2 Deficiency Attenuates Cardiac Fibrosis: Insights From Single-Cell Transcriptomics in Pkp2-Knockout Rats. [Abstract]

2025 Sep 18;6(10):e70392. PMID: 40979216
Cell Death Dis

Matrix stiffness maintains bladder cancer stemness via integrin-nuclear skeleton axis. [Abstract]

2025 Dec 12;16(1):887. PMID: 41387479
Int J Biol Macromol

SUMO-specific protease 2 governs cardiac function through the regulation of β-catenin SUMOylation. [Abstract]

2026 Jun:369:152797. PMID: 42219101
Acta Pharmacol Sin

Galangin 3-methyl ether alleviates mouse hypertrophic cardiomyopathy via targeting HDAC2 and subsequently inactivating the PI3K-AKT signaling pathway. [Abstract]

2025 Nov 1. PMID: 41176539
Phytomedicine

18β-Glycyrrhetinic acid from Glycyrrhiza Uralensis protects colonic epithelium in ulcerative colitis by activating Wnt/β-catenin pathway to restore tight junction. [Abstract]

2025 May 4:143:156830. PMID: 40450977
Apoptosis

SEMA3E promotes beige adipocyte differentiation and thermogenesis via β-catenin signaling in mice. [Abstract]

2026 Feb 2;31(2):63. PMID: 41627586
Chemosphere

Benzophenone induces cardiac developmental toxicity in zebrafish embryos by upregulating Wnt signaling. [Abstract]

2023 Dec:344:140283. PMID: 37775055
Sci Total Environ

Activation of the Wnt/β-catenin pathway attenuates the acute toxic effects of copper exposure in Penaeus vannamei. [Abstract]

2025 Dec 18:1012:181191. PMID: 41418521
Sci Total Environ

Hematopoietic stem cell and immunotoxicity in zebrafish embryos induced by exposure to Metalaxyl-M. [Abstract]

2022 Feb 25;809:152102. PMID: 34863748
J Orthop Transl

Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote critical size rat calvarial defect healing by enhanced osteogenesis and VEGF-mediated angiogenesis. [Abstract]

2020 Mar 28;23:29-37. PMID: 32477867
Biomed Pharmacother

Potential effects of bexarotene on neural development and function in zebrafish embryos. [Abstract]

2026 Feb:195:119050. PMID: 41579708
Stem Cell Res Ther

Beclin1 deficiency unlocks cardiac lineage commitment through convergent Wnt and BMP signaling activation. [Abstract]

2025 Nov 13;16(1):636. PMID: 41233909
Aging Cell

Lipoteichoic acid restrains macrophage senescence via β-catenin/FOXO1/REDD1 pathway in age-related osteoporosis. [Abstract]

2024 Mar;23(3):e14072. PMID: 38126583
J Genet Genomics

Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification. [Abstract]

2022 Dec;49(12):1101-1113. PMID: 36460297
J Cell Biol

Bistable Otx2-Id1 circuitry governs a developmental transition state in the pluripotency continuum. [Abstract]

2026 Jun 1;225(6):e202504054. PMID: 41995726
J Agric Food Chem

α-Ketoglutarate Restores Intestinal Barrier Function through Promoting Intestinal Stem Cells-Mediated Epithelial Regeneration in Colitis. [Abstract]

2022 Nov 2;70(43):13882-13892. PMID: 36269035
Cell Biosci

Label-free quantitative proteomics identifies transforming growth factor β1 (TGF-β1) as an inhibitor of adipogenic transformation in OP9-DL1 cells and primary thymic stromal cells. [Abstract]

2019 Jun 14:9:48. PMID: 31249661
JCI Insight

WNT signaling contributes to the extrahepatic bile duct proliferative response to obstruction in mice. [Abstract]

2024 Dec 5:e181857. PMID: 39636699
Cancer Cell Int

Silencing expression of PHF14 in glioblastoma promotes apoptosis, mitigates proliferation and invasiveness via Wnt signal pathway. [Abstract]

2019 Nov 27;19:314. PMID: 31798343
Biochem Pharmacol

Extracellular vesicles from bone marrow mesenchymal stem cells alleviate osteoporosis in mice through USP7-mediated YAP1 protein stability and the Wnt/β-catenin pathway. [Abstract]

2023 Nov:217:115829. PMID: 37748664
Chem Biol Interact

Modeling diabetic cardiomyopathy using human cardiac organoids: Effects of high glucose and lipid conditions. [Abstract]

2025 Apr 25:411:111421. PMID: 39984109
Commun Biol

Enhancing reprogramming towards induced human expanded pluripotency through substitution of SOX2 with engineered SOX17 transcription factors. [Abstract]

2026 Apr 9;9(1):780. PMID: 41957290
Life Sci

Bexarotene regulates zebrafish embryonic development by activating Wnt signaling pathway. [Abstract]

2025 Apr 25:123664. PMID: 40288573
Int Immunopharmacol

Curcumin activates the Wnt/β-catenin signaling pathway to alleviate hippocampal neurogenesis abnormalities caused by intermittent hypoxia: A study based on network pharmacology and experimental verification. [Abstract]

2024 Oct 2;143(Pt 1):113299. PMID: 39362017
Toxicology

Dimethyl fumarate induces cardiac developmental toxicity in zebrafish via down-regulation of oxidative stress. [Abstract]

2024 Mar:503:153735. PMID: 38272385
Mol Neurobiol

Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis. [Abstract]

2024 Apr;61(4):1936-1952. PMID: 37819429
FASEB J

Serpina3c deficiency induced necroptosis promotes non-alcoholic fatty liver disease through β-catenin/Foxo1/TLR4 signaling. [Abstract]

2022 May;36(5):e22316. PMID: 35429042
iScience

Evolution and Developmental Diversity of Skin Spines in Pufferfishes. [Abstract]

2019 Sep 27;19:1248-1259. PMID: 31353167
Neurochem Int

Cordycepin alleviates aluminium chloride-induced neurodevelopmental toxicity in zebrafish embryos by modulating inflammation and Wnt signaling. [Abstract]

2026 Sep:198:106207. PMID: 42314834
Neurochem Int

Inhibition of Wnt/β-catenin signaling attenuates axonal degeneration in models of Parkinson's disease. [Abstract]

2022 Oct:159:105389. PMID: 35809720
Oncol Rep

Salinomycin induces apoptosis and differentiation in human acute promyelocytic leukemia cells. [Abstract]

2018 Aug;40(2):877-886. PMID: 29989650

IWR-1 purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2018 Aug;40(2):877-886. [Abstract]

IWR-1 induces cell differentiation. (A) NB4 and (B) HL-60 cells are treated with 0 (DMSO), 5 and 10 μM IWR-1 for three days, and the expression of β-catenin and differentiation marker CD11b is assessed by western blotting.
Cell Signal

Serpina3c regulates adipose differentiation via the Wnt/β-catenin-PPARγ pathway. [Abstract]

2022 May:93:110299. PMID: 35263629
Aquacul Rep

2021, 100774.
Bone

Macrophage migration inhibitory factor promotes heterotopic ossification by mediating ROS/HIF-1α positive feedback loop and activating Wnt/β-catenin signaling pathway. [Abstract]

2024 Nov 14:117331. PMID: 39549900
J Cell Sci

Tbl1 promotes Wnt/β-catenin signaling-induced degradation of the Tcf7l1 protein in mouse ESCs. [Abstract]

2024 May 1;137(9):jcs261241. PMID: 38639717
Exp Cell Res

Up-regulation of Dsg2 confered stem cells with malignancy through wnt/β-catenin signaling pathway. [Abstract]

2022 Nov 11;422(1):113416. PMID: 36375513
Anim Reprod Sci

Pharmacological activation of Wnt/β-catenin signaling is essential for oocyte developmental competence and enhances in vitro embryo production in water buffalo. [Abstract]

2025 Dec 4:285:108070. PMID: 41365046
Stem Cells Int

Long Noncoding RNA EMX2-AS Facilitates Osteoblast Differentiation and Bone Formation by Inhibiting EMX2 Protein Translation and Activating Wnt/ β-Catenin Pathway. [Abstract]

2024 Nov 26:2024:4397807. PMID: 39628661
Hum Mol Genet

TRIM25 activates Wnt/β-catenin signalling by destabilising MAT2A mRNA to drive thoracic aortic aneurysm development. [Abstract]

2024 Aug 31:ddae122. PMID: 39216871
Cell Biol Int

SFRP2 enhanced the adipogenic and neuronal differentiation potentials of stem cells from apical papilla. [Abstract]

2017 May;41(5):534-543. PMID: 28244619

IWR-1 purchased from MedChemExpress. Usage Cited in: Cell Biol Int. 2017 May;41(5):534-543. [Abstract]

SCAPs are cultured with modified neural differentiation medium for the indicated time periods. The neuron-like cells in control group, SFRP2 over-expressed group and 10 uM IWR1-endo treated group are positive for βIII-tubulin (red) expression by using immunofluorescence staining.
Ren Fail

Intermedin protects HUVECs from ischemia reperfusion injury via Wnt/β-catenin signaling pathway. [Abstract]

2019 Nov;41(1):159-166. PMID: 30931679
Exp Eye Res

The effects of pigment epithelium-derived factor and associated peptides on the differentiation of retinal ganglion cells from human-induced pluripotent stem cells. [Abstract]

2025 May 21:110440. PMID: 40409357
Animals (Basel)

The Effect of Inhibiting the Wingless/Integrated (WNT) Signaling Pathway on the Early Embryonic Disc Cell Culture in Chickens. [Abstract]

2024 May 4;14(9):1382. PMID: 38731386
Tissue Cell

Curcumin triggers the Wnt/β-catenin pathway and shields neurons from injury caused by intermittent hypoxia. [Abstract]

2024 Dec:91:102587. PMID: 39454474
Mol Biotechnol

Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development. [Abstract]

2024 Feb;66(2):208-221. PMID: 37071303
Theriogenology

Effect of VD3 on cell proliferation and the Wnt signaling pathway in bovine endometrial epithelial cells treated with lipopolysaccharide. [Abstract]

2022 Nov:193:68-76. PMID: 36156426
Biochem Biophys Res Commun

3D layered co-culture model enhances Trastuzumab Deruxtecan sensitivity and reveals the combined effect with G007-LK in HER2-positive non-small cell lung cancer. [Abstract]

2024 Jun 11:725:150255. PMID: 38897043
Cytotechnology

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis. [Abstract]

2021 Apr;73(2):203-215. PMID: 33911345
Cell Press Blue

2026 Apr 22.
Seoul National University

2029.
SSRN

2025 Sep 30.
SSRN

2024 Apr 30.
Research Square Print

September 27th, 2022.
Ann Transl Med

Activation of the AKT/GSK-3β/β-catenin pathway via photobiomodulation therapy promotes neural stem cell proliferation in neonatal rat models of hypoxic-ischemic brain damage. [Abstract]

2022 Jan;10(2):55. PMID: 35282079
Oxid Med Cell Longev

Study on Protection of Human Umbilical Vein Endothelial Cells from Amiodarone-Induced Damage by Intermedin through Activation of Wnt/ β-Catenin Signaling Pathway. [Abstract]

2021 Aug 14:2021:8889408. PMID: 34434487
Research Square Preprint

2021 Jun.
Curr Protoc Cell Biol

An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids. [Abstract]

2018 Dec;81(1):e62. PMID: 30239150

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (122.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4424 mL

12.2118 mL

24.4236 mL

5 mM

0.4885 mL

2.4424 mL

4.8847 mL

10 mM

0.2442 mL

1.2212 mL

2.4424 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
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The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

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Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (289 KB)
SDS (394 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Sara R. Martins-Neves, et al., IWR-1, a tankyrase inhibitor, attenuates Wnt/β-catenin signaling in cancer stem-like cells and inhibits in vivo the growth of a subcutaneous human osteosarcoma xenograft, Cancer Letters, Volume 414, 2018, Pages 1-15, ISSN 0304-3835. [Content Brief]

[2]. Lee SC, et al., IWR-1 inhibits epithelial-mesenchymal transition of colorectal cancer cells through suppressing Wnt/β-catenin signaling as well as survivin expression. Oncotarget. 2015 Sep 29;6(29):27146-59.

[3]. Wang, W. M., et al., IWR-1 attenuates the promotional effect of IL-36γ in a mouse model of psoriasis. BMC immunology, 25(1), 78. [Content Brief]

[4]. Chen, B., Dodge, M.E., Tang, W., et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat. Chem. Biol. 5(2), 100-107 (2009). [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4424 mL	12.2118 mL	24.4236 mL	61.0590 mL
	5 mM	0.4885 mL	2.4424 mL	4.8847 mL	12.2118 mL
	10 mM	0.2442 mL	1.2212 mL	2.4424 mL	6.1059 mL
	15 mM	0.1628 mL	0.8141 mL	1.6282 mL	4.0706 mL
	20 mM	0.1221 mL	0.6106 mL	1.2212 mL	3.0530 mL
	25 mM	0.0977 mL	0.4885 mL	0.9769 mL	2.4424 mL
	30 mM	0.0814 mL	0.4071 mL	0.8141 mL	2.0353 mL
	40 mM	0.0611 mL	0.3053 mL	0.6106 mL	1.5265 mL
	50 mM	0.0488 mL	0.2442 mL	0.4885 mL	1.2212 mL
	60 mM	0.0407 mL	0.2035 mL	0.4071 mL	1.0177 mL
	80 mM	0.0305 mL	0.1526 mL	0.3053 mL	0.7632 mL
	100 mM	0.0244 mL	0.1221 mL	0.2442 mL	0.6106 mL

Keywords

IWR-1

67 Cited Publications

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IWR-1

Immunology/Inflammation Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Antihypertensive Compound Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Publications Citing Use of MedChemExpress (MCE) IWR-1

Voir tous les produits spécifiques à Isoform Wnt

Activité biologique

Chemical Information

Publications (67)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

IWR-1

Immunology/Inflammation Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Antihypertensive Compound Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Publications Citing Use of MedChemExpress (MCE) IWR-1

Voir tous les produits spécifiques à Isoform Wnt

Activité biologique

Chemical Information

Publications (67)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

IWR-1 Related Classifications

HY-12238 Related Classifications