Cat. No.: HY-14302

Salmeterol

(Synonyms: GR33343X)

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Salmeterol (GR33343X) is a potent and selective human β2 adrenoceptor agonist. Salmeterol shows potent stimulation of cAMP accumulation in CHO cells expressing human β2, β1 and β3 adrenoceptors with pEC₅₀s of 9.6, 6.1, and 5.9, respectively.

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Pureté: 99.69%
CAS No.: 89365-50-4
Formule: C₂₅H₃₇NO₄
Masse moléculaire:415.57

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Salmeterol Chemical Structure

CAS No.: 89365-50-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Essai clinique
Chemical Information
Publications (8)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]

β2 adrenoceptor

9.6 (pEC₅₀)

β1 adrenoceptor

6.1 (pEC₅₀)

β3 adrenoceptor

5.9 (pEC₅₀)

Voir tous les produits spécifiques à Isoform Adrenergic Receptor :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.07 nM Compound: Salmeterol	Agonist activity at human recombinant beta2 adrenergic receptor expressed in CHO cells assessed as elevation in cAMP level after 1 hr by flashplate method Agonist activity at human recombinant beta2 adrenergic receptor expressed in CHO cells assessed as elevation in cAMP level after 1 hr by flashplate method	[PMID: 20804199]
CHO	EC₅₀	0.07 nM Compound: salmeterol	Agonist activity at human recombinant beta-2 adrenoceptor expressed in CHO cells assessed as elevation of cAMP Agonist activity at human recombinant beta-2 adrenoceptor expressed in CHO cells assessed as elevation of cAMP	[PMID: 17498952]
CHO	EC₅₀	0.07 nM Compound: salmeterol	Agonist activity at human recombinant adrenergic beta-2 receptor expressed in CHO cells assessed as elevation in cAMP levels Agonist activity at human recombinant adrenergic beta-2 receptor expressed in CHO cells assessed as elevation in cAMP levels	[PMID: 17897826]
HEK293	EC₅₀	0.33 nM Compound: 2	Agonist activity at human beta2-adrenoceptor expressed in HEK cells assessed as increase of cAMP level after 10 mins by radioimmunoassay Agonist activity at human beta2-adrenoceptor expressed in HEK cells assessed as increase of cAMP level after 10 mins by radioimmunoassay	[PMID: 21310610]

In Vitro

Salmeterol (0.001-25 μM) inhibits human T lymphocyte proliferation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

Human T lymphocytes (THP-1 cells)
Concentration:

0.001, 0.01, 0.05, 0.2, 1, 5, and 25 µM
Incubation Time:
Result:

The proliferation of Th2 cells was inhibited in a concentration dependent manner.

In Vivo

Salmeterol (0.16 mg/kg), Formoterol (0.32 mg/kg) and combined treatment have therapeutic effects in mice with chronic obstructive pulmonary disease (COPD)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6 mice (6-8 weeks old, body weight: 32-35 g)^[3]
Dosage:

Salmeterol (0.16 mg/kg) and/or Formoterol (0.32 mg/kg)
Administration:

The therapeutic efficacy of co-treatment was investigated in this model over a 56-day-long observation period.
Result:

COPD assessment test scores were markedly improved in mice with COPD.

Essai clinique

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 89365-50-4
Appearance Solid
Masse moléculaire 415.57
Formule C₂₅H₃₇NO₄
Color White to off-white
SMILES

OC1=CC=C(C(O)CNCCCCCCOCCCCC2=CC=CC=C2)C=C1CO
Synonyms

GR33343X
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

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Journal Impact Factor

Nat Commun

DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. [Abstract]

2020 Sep 25;11(1):4857. PMID: 32978402
Neuron

Structural basis of psychedelic LSD recognition at dopamine D1 receptor. [Abstract]

2024 Jul 25:S0896-6273(24)00494-X. PMID: 39094559
Mol Ther

Renal tubular epithelial IGFBP7 interacts with PKM2 to drive renal lipid accumulation and fibrosis. [Abstract]

2025 May 8:S1525-0016(25)00382-X. PMID: 40346800
J Pharm Anal

Baicalin reduces chronic stress-induced breast cancer metastasis via directly targeting β2-adrenergic receptor. [Abstract]

2024 Jul;14(7):100934. PMID: 39139999

Salmeterol purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2024 Jul;14(7):100934. [Abstract]

MDA-MB-231 and 4T1 cells were treated with BA, isoproterenol (ISO)/Salmeterol (SAL), or BA plus ISO/SAL, and the intracellular cyclic adenosine monophosphate (cAMP) level was detected.

Salmeterol purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2024 Jul;14(7):100934. [Abstract]

The protein expression levels of EMT markers (E-cadherin, N-cadherin, MMP2, and vimentin) were detected in 4T1 cells treated with BA, Salmeterol (SAL), or SAL combined with BA.
Cell Rep

Structural Basis of the Diversity of Adrenergic Receptors. [Abstract]

2019 Dec 3;29(10):2929-2935.e4 PMID: 31801060
Neurobiol Dis

Neonatal therapy with clenbuterol and salmeterol restores spinogenesis and dendritic complexity in the dentate gyrus of the Ts65Dn model of Down syndrome. [Abstract]

2020 Jul;140:104874. PMID: 32325119
J Pharm Biomed Anal

Screening method of mildronate and over 300 doping agents by reversed-phase liquid chromatography-high resolution mass spectrometry. [Abstract]

2021 Feb 20:195:113870. PMID: 33453569
Drug Test Anal

High-throughput liquid chromatography tandem mass spectrometry assay as initial testing procedure for analysis of total urinary fraction. [Abstract]

2021 Feb;13(2):283-298. PMID: 32852861

Solvant et solubilité

In Vitro:

DMSO : ≥ 100 mg/mL (240.63 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4063 mL

12.0317 mL

24.0633 mL

5 mM

0.4813 mL

2.4063 mL

4.8127 mL

10 mM

0.2406 mL

1.2032 mL

2.4063 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Panayiotis A Procopiou, et al. The discovery of long-acting saligenin β₂ adrenergic receptor agonists incorporating a urea group. Bioorg Med Chem. 2011 Oct 15;19(20):6026-32. [Content Brief]

[2]. Malcolm Johnson. Effects of beta2-agonists on resident and infiltrating inflammatory cells. J Allergy Clin Immunol. 2002 Dec;110(6 Suppl):S282-90. [Content Brief]

[3]. Zhiyuan Wang, et al. Efficacy of salmeterol and formoterol combination treatment in mice with chronic obstructive pulmonary disease. Exp Ther Med. 2018 Feb;15(2):1538-1545. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4063 mL	12.0317 mL	24.0633 mL	60.1583 mL
	5 mM	0.4813 mL	2.4063 mL	4.8127 mL	12.0317 mL
	10 mM	0.2406 mL	1.2032 mL	2.4063 mL	6.0158 mL
	15 mM	0.1604 mL	0.8021 mL	1.6042 mL	4.0106 mL
	20 mM	0.1203 mL	0.6016 mL	1.2032 mL	3.0079 mL
	25 mM	0.0963 mL	0.4813 mL	0.9625 mL	2.4063 mL
	30 mM	0.0802 mL	0.4011 mL	0.8021 mL	2.0053 mL
	40 mM	0.0602 mL	0.3008 mL	0.6016 mL	1.5040 mL
	50 mM	0.0481 mL	0.2406 mL	0.4813 mL	1.2032 mL
	60 mM	0.0401 mL	0.2005 mL	0.4011 mL	1.0026 mL
	80 mM	0.0301 mL	0.1504 mL	0.3008 mL	0.7520 mL
	100 mM	0.0241 mL	0.1203 mL	0.2406 mL	0.6016 mL

Keywords

Salmeterol

8 Cited Publications

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Salmeterol

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Publications Citing Use of MedChemExpress (MCE) Salmeterol

Voir tous les produits spécifiques à Isoform Adrenergic Receptor

Activité biologique

Chemical Information

Publications (8)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Salmeterol Related Classifications

HY-14302 Related Classifications