BMS-243117
BMS-243117 is a potent, and selective benzothiazole based p56Lck inhibitor with an IC50 of 4 nM. BMS-243117 inhibits anti-CD3/anti-CD28 induced PBL (human peripheral blood T-cells) proliferation with an IC50 of 1.1 μM. BMS-243117 binds in an extended conformation to the ATP-binding site of Lck.
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- CAS No.: 225521-80-2
- Formule: C20H21ClN4O2S
- Masse moléculaire:416.92
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
Lck 4 nM (IC50) |
Fyn 32 nM (IC50) |
Blk 84 nM (IC50) |
Src 158 nM (IC50) |
Hck 960 nM (IC50) |
Lyn 330 nM (IC50) |
Fgr 60 nM (IC50) |
Chemical Information
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CAS No. 225521-80-2
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Masse moléculaire 416.92
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Formule C20H21ClN4O2S
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SMILES
O=C(C1=CC=C2N=C(NC(NC(C)(C)C)=O)SC2=C1)NC3=C(C)C=CC=C3Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BMS-243117 Related Classifications
HY-120622 Related Classifications
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