MC-25B
MC-25B is a DCAF16-recruiting FKBP12 PROTAC degrader with a DC50 of 0.35 μM. MC-25B promotes the formation of a ternary complex with DCAF16 and nuclear-localized FKBP12_NLS, thereby mediating DCAF16-dependent degradation of FKBP12_NLS via the proteasome and Cullin-RING ligase pathway.
(Pink: FKBP12 ligand (HY-170988); Blue: DCAF16 ligand (HY-170986); Black: linker (HY-128844)).
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Formule: C65H92ClN7O14
- Masse moléculaire:1230.92
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
|
FKBP12 0.35 μM (DC50) |
MC-25B (1 μM; 24 h) induces selective, DCAF16-dependent degradation of FKBP12 in HEK293T cells expressing FLAG-FKBP12_NLS, with only one identified DCAF16-independent off-target effect[1].
MC-25B (0.1-5 μM; 8 h) induces dose-dependent degradation of FLAG-FKBP12_NLS in HEK293T cells overexpressing HA-DCAF16, with a DC50 of 0.35 μM and a Dmax of 89%[1].
MC-25B (1-2 μM; 8 h) induces the degradation of FLAG-FKBP12_NLS in HEK293T cells expressing the DCAF16_3Cys mutant (retaining only C177−179), confirming that this cysteine cluster plays a critical role in MC-25B-mediated degradation[1].
MC-25B (incubated for 72 h) inhibits the viability of HEK293T cells, with an IC50 value of 2.2 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HEK293T cells overexpressing HA-DCAF16 and FLAG-FKBP12_NLS
-
Concentration:0.1 μM, 0.2 μM, 0.5 μM, 1 μM, 2 μM, 5 μM
-
Incubation Time:8 h
-
Result:Induced dose-dependent degradation of FLAG-FKBP12_NLS, with a maximum degradation (Dmax) of 89% and a half-maximal degradation concentration (DC50) of 0.35 μM.
Chemical Information
-
Masse moléculaire 1230.92
-
Formule C65H92ClN7O14
-
SMILES
O=C(CN1CCN(C2=CC=CC(C(N3CCC(N(C4CC4)C(CCl)=O)CC3)=O)=C2)CC1)NCCCOCCOCCOCCCNC(COC5=CC=CC([C@H](OC([C@@H]6CCCCN6C(C(C(C)(C)CC)=O)=O)=O)CCC7=CC(OC)=C(OC)C=C7)=C5)=O
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MC-25B Related Classifications
HY-170983 Related Classifications
Powered by Bioz