Mersalyl
Based on 1 Customer Validation
Mersalyl (Salirgan) is a potent vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 (HIF-1) inducer. Mersalyl induces VEGF and ENO1 mRNA expression. Mersalyl shows diuresis effects.
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- Pureté : 89.25%
- CAS No.: 492-18-2
- Formule: C13H16HgNNaO6
- Masse moléculaire:505.85
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Stockage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
Mersalyl (100 µM) induces VEGF and ENO1 mRNA expression but inhibits EPO mRNA expression induced by hypoxia, DFO, or CoCl2[2].
Mersalyl (20 µM; 5 min) is a cell-impermeant organomercurial compound that reacts with free thiol groups in mitochondrial membrane protein[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hep3B cells
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Concentration:100 µM
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Incubation Time:6 h
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Result:Induced VEGF and ENO1 mRNA expression but inhibited EPO mRNA expression induced by hypoxia, DFO, or CoCl2.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rats[3]
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Dosage:2.5-40 mg/kg
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Administration:Intramuscularly injection
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Result:Significantly depressed in sulfhydryl concentration at 5 mg/kg.
Chemical Information
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CAS No. 492-18-2
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Appearance Solid
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Masse moléculaire 505.85
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Formule C13H16HgNNaO6
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Color White to off-white
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SMILES
[O-]C(COC1=C(C(NCC2OC)=[O][Hg+2]([CH2-]2)[OH-])C=CC=C1)=O.[Na+]
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Synonyms
Salirgan; Salurin; Salyrgan
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (197.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Kowaltowski AJ, et al. Mitochondrial membrane protein thiol reactivity with N-ethylmaleimide or mersalyl is modified by Ca2+: correlation with mitochondrial permeability transition. Biochim Biophys Acta. 1997 Feb 15;1318(3):395-402. [Content Brief]
[2]. Agani F, et al. Mersalyl is a novel inducer of vascular endothelial growth factor gene expression and hypoxia-inducible factor 1 activity. Mol Pharmacol. 1998 Nov;54(5):749-54. [Content Brief]
[3]. FARAH A, et al. Histochemical studies on the site of action of mercurial diuretics. J Histochem Cytochem. 1955 Jul;3(4):271-3. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9769 mL | 9.8844 mL | 19.7687 mL | 49.4218 mL |
| 5 mM | 0.3954 mL | 1.9769 mL | 3.9537 mL | 9.8844 mL | |
| 10 mM | 0.1977 mL | 0.9884 mL | 1.9769 mL | 4.9422 mL | |
| 15 mM | 0.1318 mL | 0.6590 mL | 1.3179 mL | 3.2948 mL | |
| 20 mM | 0.0988 mL | 0.4942 mL | 0.9884 mL | 2.4711 mL | |
| 25 mM | 0.0791 mL | 0.3954 mL | 0.7907 mL | 1.9769 mL | |
| 30 mM | 0.0659 mL | 0.3295 mL | 0.6590 mL | 1.6474 mL | |
| 40 mM | 0.0494 mL | 0.2471 mL | 0.4942 mL | 1.2355 mL | |
| 50 mM | 0.0395 mL | 0.1977 mL | 0.3954 mL | 0.9884 mL | |
| 60 mM | 0.0329 mL | 0.1647 mL | 0.3295 mL | 0.8237 mL | |
| 80 mM | 0.0247 mL | 0.1236 mL | 0.2471 mL | 0.6178 mL | |
| 100 mM | 0.0198 mL | 0.0988 mL | 0.1977 mL | 0.4942 mL |
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