Coenzyme A
Based on 2 publication(s) in Google Scholar
Coenzyme A (CoASH) is a ubiquitous and essential cofactor, which is an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism. Coenzyme A plays a central role in the oxidation of pyruvate in the citric acid cycle and the metabolism of carboxylic acids, including short- and long-chain fatty acids.
For research use only. We do not sell to patients.
- Purity: 96.81%
- CAS No.: 85-61-0
- Formula: C21H36N7O16P3S
- Molecular Weight:767.53
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Coenzyme A
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Customer Validation & Images
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WB
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IHC
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Flow Cytometry
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IHC
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WB
All Endogenous Metabolite Isoforms
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Biological Activity
Coenzyme A functions to generate metabolically active thioesters, while it also has the potential to act as a low-molecular-weight antioxidant in response to oxidative and metabolic stress in exponentially growing bacteria[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 85-61-0
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Appearance Solid
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Molecular Weight 767.53
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Formula C21H36N7O16P3S
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Color White to light yellow
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SMILES
CC([C@H](C(NCCC(NCCS)=O)=O)O)(C)COP(OP(OC[C@H]1O[C@@H](N2C3=NC=NC(N)=C3N=C2)[C@H](O)[C@@H]1OP(O)(O)=O)(O)=O)(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (2)
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Journal Impact Factor
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Most Recent
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Nature
2025 Nov;647(8090):706-715. PMID: 41034585
Coenzyme A purchased from MedChemExpress. Usage Cited in: Nature. 2025 Nov;647(8090):706-715. [Abstract]
CoA was provided through the drinking water at 500 mg/L and i.p. injection (50 mg/kg) three times per week for 30 days. Immunoblots detection for HMGCS2 in small intestinal crypts from control and CoA-treated mice.
Coenzyme A purchased from MedChemExpress. Usage Cited in: Nature. 2025 Nov;647(8090):706-715. [Abstract]
CoA was provided through the drinking water at 500 mg/L and i.p. injection (50 mg/kg) three times per week for 30 days. IHC detection for HMGCS2 in small intestinal crypts from control and CoA-treated mice. Scale bar, 20 µm.
Coenzyme A purchased from MedChemExpress. Usage Cited in: Nature. 2025 Nov;647(8090):706-715. [Abstract]
Flow cytometry and quantification of IL-22 expression in CD8αβ+ cells after 24 h of CoA (500 µM) treatment (n = 8 per group).
Coenzyme A purchased from MedChemExpress. Usage Cited in: Nature. 2025 Nov;647(8090):706-715. [Abstract]
CoA was provided through the drinking water at 500 mg/L and i.p. injection (50 mg/kg) three times per week for 30 days. IHC for regenerating crypts using OLFM4 as a stem cell marker in control and CoA-treated mice.
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J Adv Res
c-Myc-PANK3-EMT axis regulates the structure and function of intestinal barrier in ulcerative colitis. [Abstract]2025 Dec 8:S2090-1232(25)00996-8. PMID: 41371401
Coenzyme A purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Dec 8:S2090-1232(25)00996-8. [Abstract]
phosphorylation level of PI3K, AKT and ERK in HT29 cells treated with TNF-α/IFN-γ combined with 250 µM CoA.
Solvent & Solubility
H2O : 100 mg/mL (130.29 mM; ultrasonic and warming and heat to 60°C)
DMSO : 100 mg/mL (130.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.63 mg/mL (2.12 mM); Clear solution
This protocol yields a clear solution of ≥ 1.63 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.63 mg/mL (2.12 mM); Clear solution
This protocol yields a clear solution of ≥ 1.63 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 25 mg/mL (32.57 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.3029 mL | 6.5144 mL | 13.0288 mL | 32.5720 mL |
| 5 mM | 0.2606 mL | 1.3029 mL | 2.6058 mL | 6.5144 mL | |
| 10 mM | 0.1303 mL | 0.6514 mL | 1.3029 mL | 3.2572 mL | |
| 15 mM | 0.0869 mL | 0.4343 mL | 0.8686 mL | 2.1715 mL | |
| 20 mM | 0.0651 mL | 0.3257 mL | 0.6514 mL | 1.6286 mL | |
| 25 mM | 0.0521 mL | 0.2606 mL | 0.5212 mL | 1.3029 mL | |
| 30 mM | 0.0434 mL | 0.2171 mL | 0.4343 mL | 1.0857 mL | |
| 40 mM | 0.0326 mL | 0.1629 mL | 0.3257 mL | 0.8143 mL | |
| 50 mM | 0.0261 mL | 0.1303 mL | 0.2606 mL | 0.6514 mL | |
| 60 mM | 0.0217 mL | 0.1086 mL | 0.2171 mL | 0.5429 mL | |
| 80 mM | 0.0163 mL | 0.0814 mL | 0.1629 mL | 0.4072 mL | |
| 100 mM | 0.0130 mL | 0.0651 mL | 0.1303 mL | 0.3257 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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