Cat. No.: HY-13524

AG-1478

(Synonyms: Tyrphostin AG-1478; NSC 693255)

51 Cited Publications

Customer Review

Based on 51 publication(s) in Google Scholar

AG-1478 (Tyrphostin AG-1478) is a selective EGFR tyrosine kinase inhibitor with IC₅₀ of 3 nM. AG-1478 has antiviral effects against HCV and encephalomyocarditis virus (EMCV).

For research use only. We do not sell to patients.

Purity: 99.57%
CAS No.: 153436-53-4
Formula: C₁₆H₁₄ClN₃O₂
Molecular Weight:315.75

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

AG-1478 Chemical Structure

CAS No.: 153436-53-4

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (51)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

AG-1478 (Tyrphostin AG-1478) is a selective EGFR tyrosine kinase inhibitor with IC₅₀ of 3 nM. AG-1478 has antiviral effects against HCV and encephalomyocarditis virus (EMCV).

IC₅₀ & Target^[1]^[4]

EGFR

3 nM (IC₅₀)

HCV

EMCV

All EGFR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	0.31 nM Compound: 31	Concentration required to inhibit the phosphorylation of a 14-residue fragment of phospholipase C gamma 1 by Epidermal growth factor receptor from A431 cell vesicles Concentration required to inhibit the phosphorylation of a 14-residue fragment of phospholipase C gamma 1 by Epidermal growth factor receptor from A431 cell vesicles	[PMID: 8568816]
A-431	IC₅₀	20 μM Compound: AG-1478	Tested for antiproliferative activity against human A431 cells Tested for antiproliferative activity against human A431 cells	[PMID: 15109642]
NIH3T3	IC₅₀	28.6 μM Compound: AG-1478	Inhibition of serum stimulated NIH3T3 cell proliferation Inhibition of serum stimulated NIH3T3 cell proliferation	[PMID: 14640561]

In Vitro

AG-1478 (AG1478) is irreversible for growth regulation of human lung (A549) and prostate (DU145) cancer cell lines, cultured in chemically defined DMEM/F12 medium. AG-1478 seems to be more effective at lower concentrations, but is unable to completely inhibit growth of A549 cells^[1]. Inhibition of EGFR by specific tyrosine kinase inhibitor AG-1478 (AG1478) significantly decreases the angiotensin II-mediated synthesis of TGF-β and fibronectin by cardiac fibroblasts. EGFR is pharmacologically inhibited by small-molecule inhibitor AG-1478 with IC₅₀ of 4 nM^[2]. Both Polyfect (PF) and Superfect (SF) treatment lead to increased apoptosis in HEK 293 cells to a similar extent as assessed by flow cytometry. The antioxidant, tempol, significantly reduced dendrimer-mediated apoptosis for both PF and SF. AG-1478 (AG1478), at a 10-fold higher dose (100 μM) than used in signaling studies, is used as a positive control and significantly induced apoptosis in HEK 293 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Administration of AG-1478 (AG1478) significantly reduces myocardial inflammation, fibrosis, apoptosis, and dysfunction in both two obese mouse models. ApoE^-/- mice are first fed with HFD for 8 weeks (ApoE-HFD), and then administrated with AG-1478 (10 mg/kg/day) or 542 (10 mg/kg/day) for another 8 weeks by oral gavage. AG-1478 or 542 treatment blocks HFD induced cardiac EGFR phosphorylation in vivo, without affecting the plasma level of low density lipoprotein (LDL) and total triglyceride (TG)^[2]. Administration of EGF (10 nM) leads to a robust and reproducible elevation in EGFR phosphorylation that can be blocked by AG-1478 (AG1478), a known inhibitor of EGFR phosphorylation. Increasing doses of Polyfect (PF) result in a significant reduction in EGF-induced EGFR phosphorylation (p<0.05) but this is to a lesser extent than observed with AG1478^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 153436-53-4
Appearance Solid
Molecular Weight 315.75
Formula C₁₆H₁₄ClN₃O₂
Color White to off-white
SMILES

COC1=CC2=NC=NC(NC3=CC=CC(Cl)=C3)=C2C=C1OC
Synonyms

Tyrphostin AG-1478; NSC 693255
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (51)

All WBIn Vivo Efficacy StudyCell Imaging/StainingIF

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Journal Impact Factor

Nat Commun

Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly. [Abstract]

2025 May 27;16(1):4887. PMID: 40425567
Nat Commun

Therapeutic potential of the secreted Kazal-type serine protease inhibitor SPINK4 in colitis. [Abstract]

2024 Jul 12;15(1):5874. PMID: 38997284

AG-1478 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 12;15(1):5874. [Abstract]

AG-1478 (10 μM; 48 h) influenced the direct downstream target of the EGFR pathway in NCM460 cells.

AG-1478 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 12;15(1):5874. [Abstract]

Morphological images and expression of proliferative cells (yellow: Ki67), Paneth cells (red: Lyz), and enteroendocrine cells (green: CgA) in organoids derived from WT and cKO mice with or without the stimulation of AG-1478 (10 μM; 48 h).

AG-1478 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jul 12;15(1):5874. [Abstract]

Typical fluorescent images of intestinal organoids from WT and KO mice including MUC2 (green), E-cadherin (red), and DAPI (blue), with AG-1478 (10 μM; 48 h) stimulation under inflammatory conditions (50 ng/mL TNF-α treatment).
Adv Sci (Weinh)

Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis. [Abstract]

2023 Jun;10(16):e2204824. PMID: 37060105

AG-1478 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Jun;10(16):e2204824. [Abstract]

ErbB4 antagonist AG‐1478 (20 nM; i.c.v.) markedly attenuated the effects of rNrg4 on food intake and body weight of HFD‐fed ErbB4 CreER mice.

AG-1478 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Jun;10(16):e2204824. [Abstract]

AG-1478 (20 nM; i.c.v.) inhibited the increased number of c-Fos⁺ cells in the PVN of ErbB4 CreER mice induced by rNrg4.
Cell Death Dis

MAL2 reprograms lipid metabolism in intrahepatic cholangiocarcinoma via EGFR/SREBP-1 pathway based on single-cell RNA sequencing. [Abstract]

2024 Jun 12;15(6):411. PMID: 38866777
Cell Death Dis

Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization. [Abstract]

2021 May 18;12(6):509. PMID: 34006822
Cell Death Dis

Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways. [Abstract]

2020 Jun 15;11(6):459. PMID: 32541671
Cell Death Dis

Protease Nexin I is a feedback regulator of EGF/PKC/MAPK/EGR1 signaling in breast cancer cells metastasis and stemness. [Abstract]

2019 Sep 9;10(9):649. PMID: 31501409
Cell Death Discov

Maternal linoleic acid-rich diet ameliorates bilirubin neurotoxicity in offspring mice. [Abstract]

2024 Jul 19;10(1):329. PMID: 39030174
Nat Struct Mol Biol

ERK-USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth. [Abstract]

2025 May;32(5):853-863. PMID: 39824978
Proc Natl Acad Sci U S A

2026 Jul 7;123(27):e2523789123. PMID: 42384696
Oncogene

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer. [Abstract]

2021 Jul;40(30):4884-4893. PMID: 34163029
Int J Biol Macromol

CXCR2, as a key regulatory gene of HDP-PG-1, maintains intestinal mucosal homeostasis. [Abstract]

2024 Jun;269(Pt 2):132025. PMID: 38704076
Int J Mol Med

IL‑37/IL‑1R8 blocks keratinocyte acantholysis via suppressing ADAM17/EGFR. [Abstract]

2026 May;57(5):122. PMID: 41823548
EMBO J

The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex. [Abstract]

2024 Apr;43(8):1388-1419. PMID: 38514807
Br J Pharmacol

CKLF-like MARVEL transmembrane domain-containing superfamily 8 (CMTM8) promotes Notch signalling to maintain CD8+ T cell- and NK cell-dependent tumour immune escape. [Abstract]

2026 May 31. PMID: 42219547
J Invest Dermatol

Cathelicidin Antimicrobial Peptide LL37 Induces TLR8 and Amplifies IL-36γ and IL-17C in Human Keratinocytes. [Abstract]

2023 May;143(5):832-841.e4. PMID: 36496195
Cancer Cell Int

Construction of a prognostic model with histone modification-related genes and identification of potential drugs in pancreatic cancer. [Abstract]

2021 Jun 5;21(1):291. PMID: 34090418
Cancer Cell Int

Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation. [Abstract]

2019 Sep 23;19:241. PMID: 31572058
Virulence

Streptococcus mutans-derived extracellular vesicles promote herpes simplex virus infection in oral epithelia. [Abstract]

2026 Dec;17(1):2602261. PMID: 41379645
J Agric Food Chem

TLR2/EGFR Are Two Sensors for pBD3 and pEP2C Induction by Sodium Butyrate Independent of HDAC Inhibition. [Abstract]

2020 Jan 15;68(2):512-522. PMID: 31870150
Ecotoxicol Environ Saf

Perinatal triphenyl phosphate exposure induces metabolic dysfunctions through the EGFR/ERK/AKT signaling pathway: Mechanistic in vitro and in vivo studies. [Abstract]

2024 Jan 1:269:115756. PMID: 38056125
Life Sci

MCP-enhanced SOD3 activity inhibits gastric cancer and potentiate chemotherapy via modulating EGFR signaling. [Abstract]

2025 Feb 1:362:123358. PMID: 39746602
Precis Clin Med

Repurposable drugs for SARS-CoV-2 and influenza sepsis with scRNA-seq data targeting post-transcription modifications. [Abstract]

2021 Aug 28;4(4):215-230. PMID: 34993416
Neurobiol Dis

ErbB1-dependent signalling and vesicular trafficking in primary afferent nociceptors associated with hypersensitivity in neuropathic pain. [Abstract]

2020 Aug;142:104961. PMID: 32531343
Front Microbiol

EGFR Activation Impairs Antiviral Activity of Interferon Signaling in Brain Microvascular Endothelial Cells During Japanese Encephalitis Virus Infection. [Abstract]

2022 Jun 30;13:894356. PMID: 35847084
ACS Omega

ATP Promotes Oral Squamous Cell Carcinoma Cell Invasion and Migration by Activating the PI3K/AKT Pathway via the P2Y2-Src-EGFR Axis. [Abstract]

2022 Oct 26;7(44):39760-39771. PMID: 36385800
Sci Rep

Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation. [Abstract]

2017 Jan 19;7:40802. PMID: 28102288

AG-1478 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2017 Jan 19;7:40802. [Abstract]

EGFR participates the process of ACh induced cell proliferation and signaling transduction. After adding 10 μM EGFR specific inhibitor AG1478 2 h prior to ACh addition, protein changes are analyzed by western blot.
Neoplasia

STAMBP promotes lung adenocarcinoma metastasis by regulating the EGFR/MAPK signaling pathway. [Abstract]

2021 Jun;23(6):607-623. PMID: 34102455
Neuropharmacology

Activation of the spinal EGFR signaling pathway in a rat model of cancer-induced bone pain with morphine tolerance. [Abstract]

2021 Sep 15:196:108703. PMID: 34260958
J Cell Mol Med

LncRNA ARAP1-AS2 promotes high glucose-induced human proximal tubular cell injury via persistent transactivation of the EGFR by interacting with ARAP1. [Abstract]

2020 Nov;24(22):12994-13009. PMID: 32969198
Oncol Rep

Upregulated CTHRC1 promotes human epithelial ovarian cancer invasion through activating EGFR signaling. [Abstract]

2016 Dec;36(6):3588-3596. PMID: 27779718
J Inflamm Res

Salvianic Acid A Regulates Ferroptosis by Activating the Nrf2-GPX4 Pathway Through EGFR to Protect Myocardial Ischemia-Reperfusion Injury. [Abstract]

2026 Jun 19:19:596461. PMID: 42345001
J Nutr

Human Milk Oligosaccharides Activate Epidermal Growth Factor Receptor and Protect Against Hypoxia-Induced Injuries in the Mouse Intestinal Epithelium and Caco2 Cells. [Abstract]

2020 Apr 1;150(4):756-762. PMID: 31915826
J Virol

Microfilaments and microtubules alternately coordinate the multi-step endosomal trafficking of Classical Swine Fever Virus. [Abstract]

2021 Feb 24;95(10):e02436-20. PMID: 33627389
Development

Inhibition of HDAC4 in granulosa cells improved co-cultured oocyte maturation in vitro independent of LH in porcine. [Abstract]

2025 Apr 1;152(7):dev204618. PMID: 40066659
Food Sci Biotechnol

Thymol promotes cell proliferation and protects against LPS-induced intestinal inflammation via activation of the EGFR/PI3K pathway. [Abstract]

2025 Feb 24;34(10):2281-2294. PMID: 40351714
J Steroid Biochem Mol Biol

Dihydrotestosterone regulation of cyclooxygenase-2 expression in bovine endometrial epithelium cells by androgen receptor mediated EGFR/PI3K/Akt pathway. [Abstract]

2021 Nov:214:106001. PMID: 34547381
J Cell Biochem

Epiregulin (EREG) is upregulated through an IL-1β autocrine loop in Caco-2 epithelial cells with reduced CFTR function. [Abstract]

2018 Mar;119(3):2911-2922. PMID: 29091309
Vet Microbiol

Curcumin maintains pseudorabies virus latent infection by inhibiting the phosphorylation level of EGFR protein. [Abstract]

2026 Jun 12:320:111114. PMID: 42296914
J Anim Physiol Anim Nutr

Dihydromyricetin Suppresses Lipopolysaccharide-Induced Intestinal Injury Through Reducing Reactive Oxygen Species Generation and NOD-Like Receptor Pyrin Domain Containing 3 Inflammasome Activation. [Abstract]

2025 Mar;109(2):610-622. PMID: 39618421
PLoS One

Morphine promotes microglial activation by upregulating the EGFR/ERK signaling pathway. [Abstract]

2021 Sep 14;16(9):e0256870. PMID: 34520454
Biochem Biophys Res Commun

Extracellular AGR2 activates neighboring fibroblasts through endocytosis and direct binding to β-catenin that requires AGR2 dimerization and adhesion domains. [Abstract]

2021 Oct 8:573:86-92. PMID: 34399098
Sex Med

Hinokiflavone alleviates high-fat diet-induced erectile dysfunction via the EGFR/PI3K/Akt/eNOS signaling pathway. [Abstract]

2025 Aug 6;13(4):qfaf059. PMID: 40808867
ChemistrySelect

2023 Jan 12.
ORL J Otorhinolaryngol Relat Spec

Human Neutrophil Elastase Mediates MUC5AC Hypersecretion via the Tumour Necrosis Factor-α Converting Enzyme-Epidermal Growth Factor Receptor Signalling Pathway in vivo. [Abstract]

2021;83(5):310-318. PMID: 34130299
bioRxiv

2023 Oct 2.
Research Square Preprint

2023 Jul 10.
Environ Toxicol

Comparative study of cigarette smoke, Klebsiella pneumoniae, and their combination on airway epithelial barrier function in mice. [Abstract]

2023 May;38(5):1133-1142. PMID: 36757011

AG-1478 purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 May;38(5):1133-1142. [Abstract]

AG1478 (2 μM) suppresses the phosphorylation of EGFR and ERK1/2 and subsequently upregulates the expression of ZO-1 and occludin in BEAS-2B cells.
bioRxiv

2023 Feb 13, 528249.
Biomed Pharmacother

PI3K inhibitor 3-MA promotes the antiproliferative activity of esomeprazole in gastric cancer cells by downregulating EGFR via the PI3K/FOXO3a pathway. [Abstract]

2022 Apr:148:112665. PMID: 35228068
Research Square Preprint

2020 Dec.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (158.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1671 mL

15.8353 mL

31.6706 mL

5 mM

0.6334 mL

3.1671 mL

6.3341 mL

10 mM

0.3167 mL

1.5835 mL

3.1671 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 1% CMC/0.5% Tween-80 in Saline water

Solubility: 5 mg/mL (15.84 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 1% CMC/0.5% Tween-80 in Saline water

Solubility: 5 mg/mL (15.84 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

DU145 (HTB-81) and A549 (CCL-185) cells are seeded on 96-well plates at concentrations of 4×10³ cells/well in MEM (DU145 cells) or DMEM (A549 cells). Following 24 h of incubation, the culture medium is replaced with serum-free DMEM/F12 (1:1) supplemented with Transferrin (5 mg/mL), sodium selenite (2 ng/mL) and albumin (0.5 mg/mL) [DMEM/F12+]. After an additional 24 h of incubation (Day 0), the medium is replaced by serum-free DMEM/F12+ medium containing tyrosine kinase inhibitors: AG494, AG-1478 respectively in concentration ranges 1-20 μM and 0.1-8 μM. The incubation is continued for the next 24 h at 37°C in a humidified atmosphere. The modified crystal violet staining method (CV) and MTT assay are used to determine the influence of the tyrphostins on the proliferation of target cells. The absorbance is measured using a Tecan multiscan plate recorder^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]^[3]

Mice^[2]
28 C57BL/6 or ApoE^-/- mice are randomly divided into four weight-matched groups. 7 mice are fed with standard animal low-fat diet containing 10 kcal.% fat, 20 kcal.% protein and 70 kcal.% carbohydrate serve as a normal control group (Control or ApoE-LF), while the remaining 21 mice are fed with high-fat diet containing 60 kcal.% fat, 20 kcal.% protein and 20 kcal.% carbohydrate for 16 weeks. Since 9^th week, AG-1478 or 542 are given daily by oral gavage at a dose of 10 mg/kg/day for the next 8 weeks. Mice in the Control and HFD groups are gavaged with vehicle (1% CMC-Na solution) only. At the day before the sacrifice of ApoE^-/- mice, doppler analysis is performed to determine the pathologic cardiac hypertrophy.
Rats^[3]
Male Wistar rats weighing about 300g are used in this study and divided into the following groups (N=5). Group 1: Non-diabetic (Control, C) animals, Group 2: C+PF (10mg/kg administered as a single intraperotoneal (i.p) injection) Group 3: C+SF (10mg/kg i.p); Group 4: C+AG-1478 (1 mg/kg i.p). Group 5: Rats bearing 4 weeks of diabetes (D) induced by a single i.p. injection of streptozotocin (55 mg/kg body weight); Group 6: D+PF (10 mg/kg i.p) Group 7: D+SF (10 mg/kg i.p) and Group 8: D+AG-1478 (1 mg/kg i.p). AG-1478 and dendrimer treatments are administered as single dose for 24h prior to sacrifice. Rat body weight and basal glucose levels are assessed before and after treatments just before sacrificing the animals. An automated blood glucose analyzer is used to assess blood glucose concentrations and rats with a blood glucose concentration above 250 mg/dL (approx. 14 mM) are declared diabetic as in previous studies.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bojko A, et al. The effect of tyrphostins AG494 and AG1478 on the autocrine growth regulation of A549 and DU145 cells. Folia Histochem Cytobiol. 2012 Jul 5;50(2):186-95. [Content Brief]

[2]. Li W, et al. EGFR Inhibition Blocks Palmitic Acid-induced inflammation in cardiomyocytes and Prevents Hyperlipidemia-induced Cardiac Injury in Mice. Sci Rep. 2016 Apr 18;6:24580. [Content Brief]

[3]. Akhtar S, et al. Cationic Polyamidoamine Dendrimers as Modulators of EGFR Signaling In Vitro and In Vivo. PLoS One. 2015 Jul 13;10(7):e0132215. [Content Brief]

[4]. Dorobantu CM, et al. Tyrphostin AG1478 Inhibits Encephalomyocarditis Virus and Hepatitis C Virus by Targeting Phosphatidylinositol 4-Kinase IIIα. Antimicrob Agents Chemother. 2016 Sep 23;60(10):6402-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1671 mL	15.8353 mL	31.6706 mL	79.1766 mL
	5 mM	0.6334 mL	3.1671 mL	6.3341 mL	15.8353 mL
	10 mM	0.3167 mL	1.5835 mL	3.1671 mL	7.9177 mL
	15 mM	0.2111 mL	1.0557 mL	2.1114 mL	5.2784 mL
	20 mM	0.1584 mL	0.7918 mL	1.5835 mL	3.9588 mL
	25 mM	0.1267 mL	0.6334 mL	1.2668 mL	3.1671 mL
	30 mM	0.1056 mL	0.5278 mL	1.0557 mL	2.6392 mL
	40 mM	0.0792 mL	0.3959 mL	0.7918 mL	1.9794 mL
	50 mM	0.0633 mL	0.3167 mL	0.6334 mL	1.5835 mL
	60 mM	0.0528 mL	0.2639 mL	0.5278 mL	1.3196 mL
	80 mM	0.0396 mL	0.1979 mL	0.3959 mL	0.9897 mL
	100 mM	0.0317 mL	0.1584 mL	0.3167 mL	0.7918 mL

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AG-1478

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Antiviral Compound Library

Differentiation Inducing Compound Library

Anti-diabetic Compound Library

Anti-Cardiovascular Disease Compound Library

Endocrinology Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Cytokine Inhibitors Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Non-natural Amino Acid Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Posttranslational Modification Library

RNA Binding Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) AG-1478

All EGFR Isoforms

Biological Activity

Chemical Information

Publications (51)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

AG-1478 Related Classifications

HY-13524 Related Classifications