dTAG-47-NEG
Based on 1 Customer Validation
dTAG-47-NEG is an inactive bifunctional negative control PROTAC and also the diastereomer of dTAGV-1 (HY-145514D). dTAG-47-NEG does not reduce the level of BCL11A-FKBP12F36V, nor does it induce the degradation of BCL11A-FKBP12F36V, proteins tagged with FKBP12F36V, wild-type FKBP12, FKBP12F36V-KRASG12V or FKBP12F36V-EWS/FLI. dTAG-47-NEG exerts no antiproliferative effect in cancer cells.
(Pink: FKBP12 ligand (HY-114420); Blue: E3 ligase ligand; Black: linker (HY-42773)).
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.93%
- Formule: C59H75N5O13
- Masse moléculaire:1062.25
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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FKBP12F36V |
dTAG-47-NEG (0.5 μM; 24 h) does not induce degradation of BCL11A-FKBP12F36V in CRBN knockout HEK293T cells cotransfected with TRIM10-CRBN CULT or TRIM58-CRBN CULT fusion constructs[1].
dTAG-47-NEG (0.15 μM) does not reactivate HBG1/2 transcription in differentiating BCL11ADDBP/DDBP HUDEP-2 cells[2].
dTAG-47-NEG does not degrade LACZ-FKBP12F36V in PATU-8902 LACZ-FKBP12F36V cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CRISPR/Cas9-generated CRBN knockout HEK293T cells
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Concentration:0.5 μM
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Incubation Time:24 h
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Result:Failed to reduce the level of BCL11A-FKBP12F36V protein.
Remained comparable to control conditions across wild-type, mutant, truncated, and empty vector cotransfection groups for both TRIM10-CRBN CULT and TRIM58-CRBN CULT systems.
Chemical Information
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Appearance Solid
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Masse moléculaire 1062.25
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Formule C59H75N5O13
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Color Light yellow to yellow
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SMILES
O=C1N(C2C(NCCC2)=O)C(C3=CC=C(NCCCCCCCCNC(COC4=C(C=CC=C4)[C@@H](CCC5=CC(OC)=C(C=C5)OC)OC([C@H]6N(CCCC6)C([C@H](C7=CC(OC)=C(C(OC)=C7)OC)CC)=O)=O)=O)C=C31)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (94.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Yang H, et al. Redirecting E3 ubiquitin ligases for targeted protein degradation with heterologous recognition domains. The Journal of biological chemistry. 2025 Jan;301(1):108077. [Content Brief]
[2]. Mehta S, et al. Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells. Cell chemical biology. 2022 Aug 18;29(8):1273-1287.e8. [Content Brief]
[3]. Nabet B, et al. Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nature communications. 2020 Sep 18;11(1):4687. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9414 mL | 4.7070 mL | 9.4140 mL | 23.5349 mL |
| 5 mM | 0.1883 mL | 0.9414 mL | 1.8828 mL | 4.7070 mL | |
| 10 mM | 0.0941 mL | 0.4707 mL | 0.9414 mL | 2.3535 mL | |
| 15 mM | 0.0628 mL | 0.3138 mL | 0.6276 mL | 1.5690 mL | |
| 20 mM | 0.0471 mL | 0.2353 mL | 0.4707 mL | 1.1767 mL | |
| 25 mM | 0.0377 mL | 0.1883 mL | 0.3766 mL | 0.9414 mL | |
| 30 mM | 0.0314 mL | 0.1569 mL | 0.3138 mL | 0.7845 mL | |
| 40 mM | 0.0235 mL | 0.1177 mL | 0.2353 mL | 0.5884 mL | |
| 50 mM | 0.0188 mL | 0.0941 mL | 0.1883 mL | 0.4707 mL | |
| 60 mM | 0.0157 mL | 0.0784 mL | 0.1569 mL | 0.3922 mL | |
| 80 mM | 0.0118 mL | 0.0588 mL | 0.1177 mL | 0.2942 mL |
dTAG-47-NEG Related Classifications
HY-147099 Related Classifications
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