1D09C3
1D09C3 is a fully human anti-HLA-DR monoclonal antibody. 1D09C3 induces apoptosis and cell death involving a cascade of events, including ROS generation, JNK activation, mitochondrial membrane depolarization, and AIF release from mitochondria. 1D09C3 shows potent anti-tumor activity and increases overall survival and median survival in JVM-2 cells and GRANTA-519 cells xenograft mice models. 1D09C3 can be used for the researches of cancer, such as chronic lymphocytic leukemia (CLL).
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 791073-97-7
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Human
HLA-DR β
1D09C3 (2.5 µg/ mL, 24 h) significantly reduces cell countings and cell survival in HLA-DR+, but not HLA-DR- cell lines[1][2].
1D09C3 (0.1-10 µg/ mL, 4-24 h) induces a potent time- and dose-dependent death of JVM-2 and GRANTA-519 cells[1].
1D09C3 (10 µg/ mL, 4-24 h) induces cell apoptosis through a caspase-independent pathway in JVM-2 and GRANTA-519 cells[1].
1D09C3 (10 µg/ mL, 5-240 min) induces mitochondrial depolarization and generation of ROS in JVM-2 and GRANTA-519 cells[1].
1D09C3 (10 µg/ mL, 0.5-4 h) induces cell death involving activation of the JNK[1].
1D09C3 (2.5 µg/ mL, 4 h) induces cell death B-CLL cell death[1][3].
1D09C3 (10 µg/ mL, 48 h) reduces cell counts by 20% for activated T cells and by 50% for resting and activated B cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HLA-DR+ cell lines JVM-2 and GRANTA-519 and the HLA-DR cell line SU-DHL-1
-
Concentration:2.5 µg/ mL
-
Incubation Time:24 h
-
Result:Reduced the absolute number of viable cells by 3-fold and 48-fold, and the survival of tumor cells by 33-fold and 25-fold for JVM-2 and GRANTA-519, respectively
-
Cell Line:JVM-2 and GRANTA-519 cells
-
Concentration:10 µg/mL
-
Incubation Time:4, 18 and 24 h
-
Result:Showed no significant change of caspase-8, 9, 3 and RARP.
-
Cell Line:GRANTA-519 cells
-
Concentration:10 μg/mL
-
Incubation Time:0.5, 1, 2 and 4 h
-
Result:Upregulated the levels of JNK in mitochondrial and AIF in cytosolic.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:JVM-2 (1 x 106)cells xenograft mice models (NOD/SCID mice, 6-8 weeks)[1]
-
Dosage:0.01 mg/mouse (day 4), 0.1 mg/mouse (day 4)
-
Administration:Intravenous injection
-
Result:Resulted in a 20% overall survival with a median survival time of 64 days.
-
Animal Model:JVM-2 (0.25 x 106) cells xenograft mice models (NOD/SCID mice, 6-8 weeks)[1]
-
Dosage:3 x 1 mg/mouse (day 4, 7, 9) and 1 mg/mouse (day 4) for treatment of early-stage disease; 6 x 1 mg/mouse at 48-hour intervars starting onday 15 for treatment of advanced-stage disease
-
Administration:Intravenous injection
-
Result:Resulted in a 100% survival at dose up to 3 mg/mouse, and a 42% overall survival with a median survival of 99 days.
-
Animal Model:GRANTA-519 cells and KMS-11 cells xenograft mice models (NOD/SCID mice, 6-8 weeks)[1][2][3]
-
Dosage:3 x 1 mg mouse (days 1, 4, and 7) for treatment of early-stage disease and 6 x 1 mg/mouse at 48-hour intervars starting onday 7 for treatment of advanced-stage disease
-
Administration:Intravenous injection
-
Result:Resulted in a significant increase of median survival (108 days), with 27% mice being alive and disease-free at the end of the 120-day observation period
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
-
Product Image
ELISA, FACS, Functional assay
Chemical Information
-
CAS No. 791073-97-7
-
SMILES
N/A
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Carlo-Stella C, et al. The anti-human leukocyte antigen-DR monoclonal antibody 1D09C3 activates the mitochondrial cell death pathway and exerts a potent antitumor activity in lymphoma-bearing nonobese diabetic/severe combined immunodeficient mice. Cancer Res. 2006 Feb 1;66(3):1799-808. [Content Brief]
[2]. Carlo-Stella C, et al. IFN-gamma enhances the antimyeloma activity of the fully human anti-human leukocyte antigen-DR monoclonal antibody 1D09C3. Cancer Res. 2007 Apr 1;67(7):3269-75. [Content Brief]
[3]. Nagy ZA, et al. Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells. Nat Med. 2002 Aug;8(8):801-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
1D09C3 Related Classifications
HY-P991590 Related Classifications
Powered by Bioz