Ganglioside GQ1b (bovine) sodium
Based on 1 Customer Validation
Ganglioside GQ1b (Tetrasialoganglioside GQ1b) (bovine) sodium is a central and peripheral nervous system ganglioside and an immunostimulator. Ganglioside GQ1b (bovine) sodium modulates NMDA receptor signaling via ERK1/2, PKA, CREB, NR2A, NR2B, and GSK3β, and increases BDNF expression. Ganglioside GQ1b (bovine) sodium reduces Aβ pathology, tau phosphorylation, and APP levels. Ganglioside GQ1b (bovine) sodium can be used for the research of Alzheimer’s disease.
For research use only. We do not sell to patients.
- Purity : 98%
- CAS No.: 68652-37-9
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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HY-W747597
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Biological Activity
Description
IC50 & Target
[1]|
Human Endogenous Metabolite |
In Vitro
Ganglioside GQ1b (0.01-10 μM; 1-12 h) sodium increases mRNA and protein expression of NMDA receptor subunits NR2A and NR2B in a time-dependent manner in H19-7 rat hippocampal cells[1].
Ganglioside GQ1b (0.01-10 μM; 12 h) sodium induces phosphorylation of CREB at Ser-133 in H19-7 rat hippocampal cells via the NMDA receptor signaling pathway[1].
Ganglioside GQ1b (1 μM; 12 h) sodium induces phosphorylation of PKA, ERK1/2, and CREB in H19-7 rat hippocampal cells via the NMDA receptor signaling pathway[1].
Ganglioside GQ1b (0.001-1 μM; 1-24 h) sodium upregulates BDNF expression in rat primary cortical neurons and restores oligomeric Aβ1-42 (HY-P1363A)-induced cell death via BDNF/TrkB signaling, while also reversing Aβ-induced BDNF reduction independent of TrkB inhibition[2].
Ganglioside GQ1b (0.01-10 μM; 1-24 h) sodium increases BDNF mRNA and protein expression in GQ1b-null SH-SY5Y human neuroblastoma cells[3].
Ganglioside GQ1b (1 μM; 1-24 h) sodium increases BDNF mRNA and protein expression in rat primary cortical neurons via the NMDA receptor signaling pathway[3].
Ganglioside GQ1b (0.1-100 µmol/L; 1-6 days) sodium dose-dependently enhances spontaneous IgG, IgM, and IgA production by human PBMCs, without altering PBMC proliferation[6].
Ganglioside GQ1b (0.01-100 µmol/L; 24 h) sodium specifically enhances IL-6 and IL-10 production by purified human T cells, while having no effect on cytokine production by human monocytes, B cells, or overall PBMC production of IL-1α, IL-1β, IL-2, or IL-4[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H19-7 rat hippocampal cells
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Concentration:0.01; 0.1; 1; 10 μM
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Incubation Time:1; 3; 6; 12 h
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Result:Increased NR2A mRNA expression by 4.2-fold relative to control.
Increased NR2B mRNA expression by 5.9-fold relative to control.
Increased NR2A protein expression by 3.2-fold relative to control.
Increased NR2B protein expression by 4.3-fold relative to control.
Elevated mRNA expression of both subunits at 6 and 12 h, with the strongest effect at 12 h.
Identified 1 μM as the most effective concentration for inducing NR2A mRNA expression.
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Cell Line:H19-7 rat hippocampal cells
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Concentration:0.01; 0.1; 1; 10 μM
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Incubation Time:12 h
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Result:Increased pCREB levels significantly relative to control, with no change in total CREB expression.
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Cell Line:H19-7 rat hippocampal cells
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Concentration:1 μM
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Incubation Time:30 min (pre-incubation); 12 h (incubation)
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Result:Increased pPKA and pERK1/2 levels relative to control.
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Cell Line:GQ1b-null SH-SY5Y human neuroblastoma cells
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Concentration:0.01; 0.1; 1; 10 μM
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Incubation Time:1; 3; 6; 12; 24 h
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Result:Increased BDNF mRNA expression most significantly at 1 μM for 12 h relative to vehicle.
Increased BDNF mRNA significantly as early as 1 h post-treatment with 1 μM GQ1b, with maximal effects between 9 and 15 h.
Increased BDNF protein levels significantly at 1 μM for 12 h and 24 h.
Increased BDNF protein expression significantly at 1 μM for 12 h via Western blotting, and co-treatment with 50 μM NeuAc2en did not alter this increase.
In Vivo
Ganglioside GQ1b (1 μg; bilateral intrahippocampal infusion; once daily; 7 days) sodium restores cognitive function of aged 3xTg-AD mice[2].
Ganglioside GQ1b (1 µg; i.c.v.; single injection) sodium increases BDNF expression 2.63-fold in the prefrontal cortex and 3.65-fold in the hippocampus of rats[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley rats (male, 7 weeks old)[1]
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Dosage:1 μg
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Administration:i.c.v.; single dose
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Result:Increased NR2A mRNA and protein expression by 1.96-fold.
Increased NR2B mRNA and protein expression by 1.74-fold.
Increased NR2B Tyr1472 phosphorylation by 1.71-fold.
Increased ERK1/2 phosphorylation by 1.46-fold.
Increased PKA phosphorylation by 2.05-fold.
Increased CREB phosphorylation by 3.1-fold.
All changes were statistically significant compared to controls.
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Animal Model:3xTg-AD mice (18-22 months old); wild-type (age-matched)[2]
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Dosage:1 μg
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Administration:bilateral intrahippocampal infusion; once daily; 7 days
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Result:Increased preferential index for novel object exploration to 68% in 3xTg-AD mice.
Increased interaction time with novel object to 14 s in 3xTg-AD mice.
Restored hippocampal BDNF protein levels to 86% of wild-type aCSF-treated levels.
Increased BDNF fluorescence intensity in CA3 region and dentate gyrus.
Increased hippocampal BDNF concentration.
Significantly increased phospho-TrkB levels compared to aCSF-infused 3xTg-AD mice.
Reduced Aβ plaque counts in CA3 region
Significantly decreased hippocampal Aβ oligomer levels, soluble Aβ1-42 and Aβ1-40 levels, and APP protein levels, while α-, β-, γ-secretase and Aβ-degrading enzyme levels remained unchanged.
Significantly reduced hippocampal phospho-tau levels at residues T181, T205, and T231 compared to aCSF-infused 3xTg-AD mice, while total tau levels remained unchanged.
Restored phospho-GSK3β levels, while PP2A levels remained unchanged.
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Animal Model:Sprague-Dawley rats (male, 7-weeks-old)[3]
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Dosage:1 µg
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Administration:i.c.v.; single injection
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Result:Increased BDNF protein expression 2.63-fold in the prefrontal cortex.
Increased BDNF protein expression 3.65-fold in the hippocampus.
Chemical Information
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CAS No. 68652-37-9
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Appearance Solid
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Color White to off-white
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SMILES
OC[C@@H](O1)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@H]3[C@H](NC(C)=O)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O[C@]5(O[C@@H]([C@H]([C@H](O[C@@]6(C[C@@H]([C@H]([C@@H](O6)[C@@H]([C@@H](CO)O)O)NC(C)=O)O)C(O[Na])=O)CO)O)[C@H](NC(C)=O)[C@@H](O)C5)C(O[Na])=O)[C@H]4O)[C@@H](O)[C@@H](CO)O3)[C@H](O[C@]7(O[C@@H]([C@H]([C@@H](CO)O[C@]8(O[C@@H]([C@@H]([C@@H](CO)O)O)[C@H](NC(C)=O)[C@@H](O)C8)C(O[Na])=O)O)[C@H](NC(C)=O)[C@@H](O)C7)C(O[Na])=O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1OC[Ceramide]
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Synonyms
Tetrasialoganglioside GQ1b sodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
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Data Sheet (318 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Jung WR, et al. The effect of ganglioside GQ1b on the NMDA receptor signaling pathway in H19-7 cells and rat hippocampus. Neuroscience. 2010 Jan 13;165(1):159-67. [Content Brief]
[2]. Shin MK, et al. Ganglioside GQ1b ameliorates cognitive impairments in an Alzheimer's disease mouse model, and causes reduction of amyloid precursor protein. Sci Rep. 2019 Jun 11;9(1):8512. [Content Brief]
[3]. Shin MK, et al. The ganglioside GQ1b regulates BDNF expression via the NMDA receptor signaling pathway. Neuropharmacology. 2014 Feb;77:414-21. [Content Brief]
[4]. Kwak DH, et al. Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells. BMB Rep. 2011 Dec;44(12):799-804. [Content Brief]
[5]. Chiba A, et al. Serum IgG antibody to ganglioside GQ1b is a possible marker of Miller Fisher syndrome. Ann Neurol. 1992 Jun;31(6):677-9. [Content Brief]
[6]. Kanda N, et al. Ganglioside GQ1b enhances Ig production by human PBMCs. J Allergy Clin Immunol. 1998 Nov;102(5):813-20. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ganglioside GQ1b (bovine) sodium Related Classifications
HY-W747599 Related Classifications
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