2-Fluoroadenosine
Based on 1 Customer Validation
2-Fluoroadenosine (2FA) is a nucleoside analogue. 2-Fluoroadenosine has antiparasite activity with EC50 value of 0.842 mM for Cryptosporidium parvum. 2-Fluoroadenosine has a highly specific structural basis for MtADK. 2-Fluoroadenosine is commonly used in the study of parasitic conditions.
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- Pureza: 99.82%
- No. CAS: 146-78-1
- Fòrmula: C10H12FN5O4
- Peso molecular:285.24
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Anti-Infection Compound Library
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Antiparasitic Compound library
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Actividad biológica
2-Fluoroadenosine (0.01-1000 µM, 24 h) exhibits strong anti Cryptosporidium activity, with an IC50 of 1.18 µM against HCT-8 host cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 146-78-1
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Appearance Solid
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Peso molecular 285.24
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Fòrmula C10H12FN5O4
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(N=C(F)N=C3N)=C3N=C2)O[C@@H]1CO
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Synonyms
2FA
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
DMSO : 100 mg/mL (350.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Arai T, et al. In vitro assessment of anticryptosporidial efficacy and cytotoxicity of adenosine analogues using a SYBR Green real-time PCR method. J Antimicrob Chemother. 2011 Mar;66(3):560-3. [Content Brief]
[2]. Reddy MCM, et al. High resolution crystal structures of Mycobacterium tuberculosis adenosine kinase: insights into the mechanism and specificity of this novel prokaryotic enzyme. J Biol Chem. 2007 Sep 14;282(37):27334-27342. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5058 mL | 17.5291 mL | 35.0582 mL | 87.6455 mL |
| 5 mM | 0.7012 mL | 3.5058 mL | 7.0116 mL | 17.5291 mL | |
| 10 mM | 0.3506 mL | 1.7529 mL | 3.5058 mL | 8.7645 mL | |
| 15 mM | 0.2337 mL | 1.1686 mL | 2.3372 mL | 5.8430 mL | |
| 20 mM | 0.1753 mL | 0.8765 mL | 1.7529 mL | 4.3823 mL | |
| 25 mM | 0.1402 mL | 0.7012 mL | 1.4023 mL | 3.5058 mL | |
| 30 mM | 0.1169 mL | 0.5843 mL | 1.1686 mL | 2.9215 mL | |
| 40 mM | 0.0876 mL | 0.4382 mL | 0.8765 mL | 2.1911 mL | |
| 50 mM | 0.0701 mL | 0.3506 mL | 0.7012 mL | 1.7529 mL | |
| 60 mM | 0.0584 mL | 0.2922 mL | 0.5843 mL | 1.4608 mL | |
| 80 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0956 mL | |
| 100 mM | 0.0351 mL | 0.1753 mL | 0.3506 mL | 0.8765 mL |
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