Amarogentin
Based on 2 publication(s) in Google Scholar
Amarogentin is a secoiridoid glycoside that is mainly extracted from Swertia and Gentiana roots. Amarogentin exhibits many biological effects, including anti-oxidative, anti-tumour, and anti-diabetic activities. Amarogentin exerts hepatoprotective and immunomodulatory effects. Amarogentin promotes apoptosis, arrests G2/M cell cycle and downregulates of PI3K/Akt/mTOR signalling pathways. Amarogentin exerts beneficial vasculo-metabolic effect by activating AMPK.
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- Pureza: 99.93%
- No. CAS: 21018-84-8
- Fòrmula: C29H30O13
- Peso molecular:586.54
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Amarogentin
MoreVer todos los productos específicos de isoformas AMPK
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Actividad biológica
Amarogentin (0-20 μM, 24 h) shows antiaging effect on yeasts, by inhibiting oxidative stress[3].
Amarogentin (0-10 μM, 24 h) inhibits H2O2-induced oxidative damage (decreased intracellular ROS level and MDA content) in the PC12 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Carbon tetrachloride (HY-Y0298)-induced liver fibrosis mice model[1]
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Dosage:100 mg/kg
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Administration:p.o.
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Result:Decreased hepatic α-SMA and TGF-β1 expression.
Inhibited phosphorylation of JNK, ERK, and p38.
Chemical Information
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No. CAS 21018-84-8
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Appearance Solid
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Peso molecular 586.54
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Fòrmula C29H30O13
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Color White to off-white
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SMILES
C=C[C@H]([C@@]1([H])C(C(OCC1)=O)=CO2)[C@@H]2O[C@@](O[C@H](CO)[C@@H](O)[C@@H]3O)([H])[C@@H]3OC(C4=C(C5=CC(O)=CC=C5)C=C(O)C=C4O)=O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Biochim Biophys Acta Mol Basis Dis
Amarogentin inhibits vascular smooth muscle cell proliferation and migration and attenuates neointimal hyperplasia via AMPK activation. [Abstract]2023 Jun;1869(5):166667. PMID: 36906074 -
Animal Model Exp Med
Anti-inflammatory effects of amarogentin on 2,4-dinitrochlorobenzene-induced atopic dermatitis-like mice and in HaCat cells. [Abstract]2023 Jun;6(3):255-265. PMID: 36131559
Solvente y solubilidad
DMSO : 100 mg/mL (170.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (3.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (3.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Zhang Y, et al. Protective Effects of Amarogentin against Carbon Tetrachloride-Induced Liver Fibrosis in Mice. Molecules. 2017 May 6;22(5). pii: E754. [Content Brief]
[2]. Wölfle U, et al. Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes. Mediators Inflamm. 2015;2015:630128. [Content Brief]
[3]. Disasa D, et al. Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress. Oxid Med Cell Longev. 2020 Aug 1;2020:3184019. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7049 mL | 8.5246 mL | 17.0491 mL | 42.6228 mL |
| 5 mM | 0.3410 mL | 1.7049 mL | 3.4098 mL | 8.5246 mL | |
| 10 mM | 0.1705 mL | 0.8525 mL | 1.7049 mL | 4.2623 mL | |
| 15 mM | 0.1137 mL | 0.5683 mL | 1.1366 mL | 2.8415 mL | |
| 20 mM | 0.0852 mL | 0.4262 mL | 0.8525 mL | 2.1311 mL | |
| 25 mM | 0.0682 mL | 0.3410 mL | 0.6820 mL | 1.7049 mL | |
| 30 mM | 0.0568 mL | 0.2842 mL | 0.5683 mL | 1.4208 mL | |
| 40 mM | 0.0426 mL | 0.2131 mL | 0.4262 mL | 1.0656 mL | |
| 50 mM | 0.0341 mL | 0.1705 mL | 0.3410 mL | 0.8525 mL | |
| 60 mM | 0.0284 mL | 0.1421 mL | 0.2842 mL | 0.7104 mL | |
| 80 mM | 0.0213 mL | 0.1066 mL | 0.2131 mL | 0.5328 mL | |
| 100 mM | 0.0170 mL | 0.0852 mL | 0.1705 mL | 0.4262 mL |
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