Ankaflavin
Based on 1 Customer Validation
Ankaflavin, isolated from Monascus-Fermented red rice, is an orally active PPARγ agonist. Ankaflavin exhibits selective cytotoxic effect and induces cell death through apoptosis on cancer cells. Ankaflavin has anti-inflammatory, anti-cancer, antiatherosclerotic, and hypolipidemic effects.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.22%
- No. CAS: 50980-32-0
- Fòrmula: C23H30O5
- Peso molecular:386.48
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
PPARγ[2].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
7.05 μM
Compound: 6
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Antiproliferative activity against human A431 cells after 72 hrs
Antiproliferative activity against human A431 cells after 72 hrs
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[PMID: 20655237] |
| IGROV-1 | IC50 |
5.08 μM
Compound: 6
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Antiproliferative activity against human IGROV1 cells after 72 hrs
Antiproliferative activity against human IGROV1 cells after 72 hrs
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[PMID: 20655237] |
| NCI-H460 | IC50 |
30 μM
Compound: 6
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Antiproliferative activity against human NCI-H460 cells after 72 hrs
Antiproliferative activity against human NCI-H460 cells after 72 hrs
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[PMID: 20655237] |
Ankaflavin (0-50 µg/mL, 48 h) shows cytotoxicity against cancer cells with no significant toxicity toward normal cells[1].
Ankaflavin (0-30 µg/mL, 0-48 h) arrests Hep G2 cell cycle at sub-G1 phase in a dose- and time-dependent manner[1].
Ankaflavin (25 µg/mL, 48 h) induces Hep G2 cell apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549, Hep G2, MRC-5 and WI-38
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Concentration:1, 10, 25, and 50 µg/mL
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Incubation Time:48 h
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Result:Showed cytotoxicity against A549 and Hep G2 cells in a dose-dependent manner with no significant toxicity toward normal cells (MRC-5 and WI-38).
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Cell Line:Hep G2 cells
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Concentration:15, 20, 25, and 30 µg/mL
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Incubation Time:12, 24, 36, and 48 h
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Result:Induced a distinct sub-G1 peak in Hep G2 cells in a dose- and time-dependent manner.
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Cell Line:Hep G2 cells
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Concentration:25 µg/mL
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Incubation Time:48 h
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Result:Exhibited significant chromatin condensation (fluorescent spot) through Hoechst staining.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats (4 weeks of age), diabetes was induced by treating them with Methylglyoxal (MG) (600 mg/kg; oral) for 4 weeks[2]
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Dosage:10 mg/kg
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Administration:Oral administration for 28 days
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Result:Exerted PPARγ agonist activity. Effectively reduced AGE (advanced glycation end-products) levels in serum, liver, and pancreas of MG-induced rats.
Chemical Information
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No. CAS 50980-32-0
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Appearance Solid
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Peso molecular 386.48
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Fòrmula C23H30O5
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Color Light yellow to yellow
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SMILES
O=C([C@@H]1C(CCCCCCC)=O)O[C@]2(C)[C@]1([H])CC(C=C(/C=C/C)OC3)=C3C2=O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
DMSO : 50 mg/mL (129.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.23 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.23 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Su NW, et al. Ankaflavin from Monascus-fermented red rice exhibits selective cytotoxic effect and induces cell death on Hep G2 cells. J Agric Food Chem. 2005 Mar 23;53(6):1949-54. [Content Brief]
[2]. Lee BH, et al. Ankaflavin: a natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. Free Radic Biol Med. 2012 Dec 1;53(11):2008-16. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5875 mL | 12.9373 mL | 25.8746 mL | 64.6864 mL |
| 5 mM | 0.5175 mL | 2.5875 mL | 5.1749 mL | 12.9373 mL | |
| 10 mM | 0.2587 mL | 1.2937 mL | 2.5875 mL | 6.4686 mL | |
| 15 mM | 0.1725 mL | 0.8625 mL | 1.7250 mL | 4.3124 mL | |
| 20 mM | 0.1294 mL | 0.6469 mL | 1.2937 mL | 3.2343 mL | |
| 25 mM | 0.1035 mL | 0.5175 mL | 1.0350 mL | 2.5875 mL | |
| 30 mM | 0.0862 mL | 0.4312 mL | 0.8625 mL | 2.1562 mL | |
| 40 mM | 0.0647 mL | 0.3234 mL | 0.6469 mL | 1.6172 mL | |
| 50 mM | 0.0517 mL | 0.2587 mL | 0.5175 mL | 1.2937 mL | |
| 60 mM | 0.0431 mL | 0.2156 mL | 0.4312 mL | 1.0781 mL | |
| 80 mM | 0.0323 mL | 0.1617 mL | 0.3234 mL | 0.8086 mL | |
| 100 mM | 0.0259 mL | 0.1294 mL | 0.2587 mL | 0.6469 mL |
Ankaflavin Related Classifications
HY-N6642 Related Classifications
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