CDr17
CDr17 is a GLUT1 substrate and selective fluorescent dye staining M1 microphages. CDr17 utilizes the Gating-Oriented Live-cell Distinction (GOLD) mechanism to enter M1 macrophages (Ex/Em = 646/662 nm).
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- No. CAS: 2991899-29-5
- Fòrmula: C39H52IN3O6
- Peso molecular:785.75
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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GLUT1 |
Guidelines (The following is our recommended protocol; this protocol serves as a guideline only and should be modified to suit your specific needs.)
1. Preparation of Stock and Working Solutions
a. Prepare a 10 mM CDr17 stock solution in anhydrous DMSO.
b. Dilute the stock solution using pre-warmed, serum-free cell culture medium or PBS to prepare a 1 μM CDr17 working solution.
Note: Please adjust the concentration of the CDr17 working solution according to your specific experimental requirements, and prepare it immediately before use.
2. Cell Staining
a. Culture cells on sterile coverslips.
b. Remove the coverslips from the culture medium and aspirate any excess medium.
c. Add the working solution, gently agitating to ensure it completely covers the cells, and incubate at room temperature for 30 minutes-1 hour.
d. Aspirate the dye working solution, then wash 2–3 times with culture medium (5 minutes per wash). Observe using a fluorescence microscope or flow cytometer.
Note: If analysis via flow cytometry is required, the cells must be detached using trypsin digestion and resuspended prior to staining.
CDr17 (1 μM; 1 h) selectively stains M1 macrophages differentiated from RAW264.7 cells with 5-fold higher intensity than M0 or M2 macrophages[1].
CDr17 (1 μM; 30 min) uptake by M1 macrophages differentiated from RAW264.7 cells is competitively inhibited by D-glucose (HY-B0389) in a dose-dependent manner, but not by L-glucose (HY-W010042)[1].
CDr17 (1 μM) uptake by M1 macrophages differentiated from RAW264.7 cells is dose-dependently inhibited by the general GLUT inhibitor Cytochalasin B (HY-16928)[1].
CDr17 (1 μM; 30 min) uptake by M1 macrophages differentiated from RAW264.7 cells is reduced by 80% in GLUT1-knockout cells, confirming GLUT1 as the target transporter[1].
CDr17 (0.5 μM; 30 min) selectively stains M1 macrophages differentiated from THP-1 human cells[1].
CDr17 selectively stains primary mouse M1 peritoneal macrophages over M0 and M2 primary macrophages[1].
CDr17 (30 min) staining intensity in HeLa cells correlates with GLUT1 expression levels, with brighter staining in GLUT1-overexpressing cells and dimmer staining in GLUT1-suppressed cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
a. Take 10 mM CDr17 (dissolved in DMSO) stock solution and dilute to 1 mM working solution with sterile PBS.
b. Sterilize the solution through a 0.2 μm filter. Use immediately or in single-use aliquots and store at -20 °C, avoiding freeze-thaw cycles and exposure to sunlight.
c. Administer CDr17 working solution intravenously at the concentration of 0.5-1 mM 15 minutes before imaging.
Note: CDr17 kinetic studies should be performed for each animal model to determine peak signal time.
CDr17 (500 μM; i.v.; single dose) selectively visualizes M1 macrophages in LPS (HY-D1056)-induced acute paw inflammation in C57BL/6J mice[1].
CDr17 (1 mM in 100 μL; i.v.; single dose) selectively visualizes inflamed joint regions in CAIA-induced rheumatoid arthritis in BALB/c mice, with fluorescence intensity correlating with disease severity[1].
CDr17 (1 mM; i.v.; single dose) selectively visualizes inflamed joint regions in CIA-induced rheumatoid arthritis in DBA/1J mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J (male, 6 to 8 weeks old)[1]
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Dosage:500 μM
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Administration:i.v.; single dose
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Result:Stained significantly more cells in LPS-injected inflamed paw tissues than in PBS-injected control tissues.
Confirmed stained cells to be M1 macrophages via anti-CD86 co-staining.
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Animal Model:BALB/c (female, 9 or 10 weeks old)[1]
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Dosage:1 mM
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Administration:i.v.; single dose
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Result:Reached fluorescence intensity peak at 15 minutes post-injection, with significantly higher intensity in inflamed joint regions of RA mice compared to control mice.
Showed a strong positive correlation (R2 = 0.8220) between joint swelling grade and CDr17 fluorescence intensity.
Confirmed CDr17 signal colocalized with CD68-positive macrophages in RA synovium.
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Animal Model:DBA/1J (female, 9 weeks old)[1]
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Dosage:1 mM
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Administration:i.v.; single dose
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Result:Successfully distinguished CIA-induced rheumatoid arthritis joints from normal control joints via selective fluorescence signal.
Chemical Information
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No. CAS 2991899-29-5
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Peso molecular 785.75
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Fòrmula C39H52IN3O6
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SMILES
O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1NC(CCCC[N+]2=C(/C=C/C=C/C=C3N(CCC)C(C=CC=C4)=C4C/3(C)C)C(C)(C)C5=C2C=CC=C5)=O.[I-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CDr17 Related Classifications
HY-D3410 Related Classifications
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