D159687
Based on 1 Customer Validation
D159687 is a selective PDE4D inhibitor.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.42%
- No. CAS: 1155877-97-6
- Fòrmula: C21H19ClN2O2
- Peso molecular:366.84
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
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PDE4 |
D159687 (1 μM, 0-24 hours) induces a transient increase in CREB phosphorylation which peaked at 6 hours after treatment[1].
D159687 (0.01-1 μM, 6 hours) causes optimal CREB phosphorylation at 1 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-22 (mouse hippocampal cell line)
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Concentration:1 μM
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Incubation Time:0, 1, 3, 6, 12, 24 hours
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Result:Induced a transient increase in CREB phosphorylation which peaked at 6 hours after treatment.
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Cell Line:HT-22 (mouse hippocampal cell line)
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Concentration:0.01 μM, 0.1 μM, 1 μM
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Incubation Time:6 hours
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Result:CREB phosphorylation was optimal at 1 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female Cynomolgus macaques (4–6 year old) [2]
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Dosage:0.05, 0.5, 5 mg/kg
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Administration:Oral daily for a week
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Result:A potential recruitment or enhancement of synaptic function with increased task difficulty.
Chemical Information
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No. CAS 1155877-97-6
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Appearance Solid
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Peso molecular 366.84
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Fòrmula C21H19ClN2O2
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Color White to off-white
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SMILES
NC(NC1=CC=C(CC2=CC(C3=CC(Cl)=CC=C3)=C(OC)C=C2)C=C1)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
DMSO : 150 mg/mL (408.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7260 mL | 13.6299 mL | 27.2598 mL | 68.1496 mL |
| 5 mM | 0.5452 mL | 2.7260 mL | 5.4520 mL | 13.6299 mL | |
| 10 mM | 0.2726 mL | 1.3630 mL | 2.7260 mL | 6.8150 mL | |
| 15 mM | 0.1817 mL | 0.9087 mL | 1.8173 mL | 4.5433 mL | |
| 20 mM | 0.1363 mL | 0.6815 mL | 1.3630 mL | 3.4075 mL | |
| 25 mM | 0.1090 mL | 0.5452 mL | 1.0904 mL | 2.7260 mL | |
| 30 mM | 0.0909 mL | 0.4543 mL | 0.9087 mL | 2.2717 mL | |
| 40 mM | 0.0681 mL | 0.3407 mL | 0.6815 mL | 1.7037 mL | |
| 50 mM | 0.0545 mL | 0.2726 mL | 0.5452 mL | 1.3630 mL | |
| 60 mM | 0.0454 mL | 0.2272 mL | 0.4543 mL | 1.1358 mL | |
| 80 mM | 0.0341 mL | 0.1704 mL | 0.3407 mL | 0.8519 mL | |
| 100 mM | 0.0273 mL | 0.1363 mL | 0.2726 mL | 0.6815 mL |
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