Referencia número: HY-146887

USP7-IN-9

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Based on 1 Customer Validation

USP7-IN-9 is a highly potent ubiquitin-specific protease 7 (USP7) inhibitor with an IC₅₀ value of 40.8 nM. USP7-IN-9 can induce apoptosis and arrest cell progression at G0/G1 and S phases in RS4; 11 cells. USP7-IN-9 reduces the protein levels of oncoproteins MDM2 and DNMT1 and increases the protein levels of tumor suppressors p53 and p21.

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Pureza: 98.10%
No. CAS: 2444374-01-8
Fòrmula: C₃₂H₃₃ClF₆N₆O₈
Peso molecular:779.08

Almacenamiento:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

USP7-IN-9 Chemical Structure

No. CAS: 2444374-01-8

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Amount: USD 0.00

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

IC₅₀: 40.8 nM (USP7)^[1]

Ver todos los productos específicos de isoformas Deubiquitinase :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	32.8 μM Compound: L55	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay	[PMID: 32497973]
Huh-7	IC₅₀	3.6 μM Compound: L55	Antiproliferative activity against human HuH-7 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay Antiproliferative activity against human HuH-7 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay	[PMID: 32497973]
LNCaP	IC₅₀	29.6 nM Compound: L55	Antiproliferative activity against human LNCaP cells assessed as reduction in cell viability incubated for 6 days by cell-titer glo assay Antiproliferative activity against human LNCaP cells assessed as reduction in cell viability incubated for 6 days by cell-titer glo assay	[PMID: 32497973]
NB-4	IC₅₀	5 μM Compound: L55	Antiproliferative activity against human NB-4 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay Antiproliferative activity against human NB-4 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay	[PMID: 32497973]
RS4-11	IC₅₀	41.6 nM Compound: L55	Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay	[PMID: 32497973]

In Vitro

USP7-IN-9 (compound L55) (0-50 μM; 3 or 6 days) exhibits inhibitory activity against cancer cells^[1].
USP7-IN-9 (1 μM; 0-72 hours) reduces the proportions of G2/M cells, and does not change the proportions of G0/G1 and S cells^[1].
USP7-IN-9 (0.1-1 μM; 24 hours) reduces the protein levels of MDM2 and DNMT1 in a dose-dependent manner, and increases the protein levels of p53 and p21^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:

LNCaP, RS4; 11, HCT 116, NB4, K562 and HuH-7 cells^[1]
Concentration:

0-50 μM
Incubation Time:

LNCaP, 6 days; RS4; 11, HCT 116, HuH-7, K562 and NB4, 3 days
Result:

Exhibited high inhibitory activity against LNCaP and RS4; 11 cells, with IC₅₀s of 29.6 nM and 41.6 nM, respectively, and weak inhibitory activity on HCT 116, NB4, K562 and HuH-7 cells.

Cell Cycle Analysis

Cell Line:

RS4; 11 cells^[1]
Concentration:

1 μM
Incubation Time:

0, 24, 48 and 72 hours
Result:

Reduced the proportions of G2/M cells, while the proportions of G0/G1 and S cells were not apparently altered.

Western Blot Analysis

Cell Line:

RS4; 11 cells^[1]
Concentration:

0.1, 0.3 and 1 μM
Incubation Time:

24 hours
Result:

Reduced the protein levels of MDM2 and DNMT1 in a dose-dependent manner, and increased the protein levels of p53 and p21.

Chemical Information

No. CAS 2444374-01-8
Appearance Solid
Peso molecular 779.08
Fòrmula C₃₂H₃₃ClF₆N₆O₈
Color Light yellow to yellow
SMILES

OC1(CN(C=NC2=C(C3=CC=C(C[NH3+])C=C3)N(C)N=C24)C4=O)CC[NH+](CC5=CC=C(C(OC)=O)C=C5Cl)CC1.O=C([O-])C(F)(F)F.O=C([O-])C(F)(F)F
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (128.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2836 mL

6.4178 mL

12.8357 mL

5 mM

0.2567 mL

1.2836 mL

2.5671 mL

10 mM

0.1284 mL

0.6418 mL

1.2836 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (273 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Li M, Liu S, Chen H, et al. N-benzylpiperidinol derivatives as novel USP7 inhibitors: Structure-activity relationships and X-ray crystallographic studies. Eur J Med Chem. 2020;199:112279. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2836 mL	6.4178 mL	12.8357 mL	32.0891 mL
	5 mM	0.2567 mL	1.2836 mL	2.5671 mL	6.4178 mL
	10 mM	0.1284 mL	0.6418 mL	1.2836 mL	3.2089 mL
	15 mM	0.0856 mL	0.4279 mL	0.8557 mL	2.1393 mL
	20 mM	0.0642 mL	0.3209 mL	0.6418 mL	1.6045 mL
	25 mM	0.0513 mL	0.2567 mL	0.5134 mL	1.2836 mL
	30 mM	0.0428 mL	0.2139 mL	0.4279 mL	1.0696 mL
	40 mM	0.0321 mL	0.1604 mL	0.3209 mL	0.8022 mL
	50 mM	0.0257 mL	0.1284 mL	0.2567 mL	0.6418 mL
	60 mM	0.0214 mL	0.1070 mL	0.2139 mL	0.5348 mL
	80 mM	0.0160 mL	0.0802 mL	0.1604 mL	0.4011 mL
	100 mM	0.0128 mL	0.0642 mL	0.1284 mL	0.3209 mL

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USP7-IN-9

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Ubiquitination Compound Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Ver todos los productos específicos de isoformas Deubiquitinase

Actividad biológica

Chemical Information

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

USP7-IN-9

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Ubiquitination Compound Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Ver todos los productos específicos de isoformas Deubiquitinase

Actividad biológica

Chemical Information

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

USP7-IN-9 Related Classifications

HY-146887 Related Classifications