Cat. No.: HY-W009338

H-Val-Tyr-OH

(Synonyms: Val-Tyr)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

H-Val-Tyr-OH (Val-Tyr) is a dipeptide formed by the connection of valine and tyrosine via a peptide bond. H-Val-Tyr-OH is a potential IKKα inhibitor and can be used in the research of cancer and inflammatory diseases.

For research use only. We do not sell to patients.

Purity: 99.11%
CAS No.: 3061-91-4
Formula: C₁₄H₂₀N₂O₄
Molecular Weight:280.32

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

H-Val-Tyr-OH Chemical Structure

CAS No.: 3061-91-4

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Biological Activity
- Description
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All IKK Isoforms :

All NF-κB Isoforms :

In Vitro

H-Val-Tyr-OH (100 μM; 5 h) can reduce the phosphorylation level of IκBα in RAW 264.7 cells stimulated with LPS (HY-D1056), thereby inhibiting the activation of the NF-κB signaling pathway, but its inhibitory effect is weaker than that of the known IKKα inhibitor BMS-345541 (HY-10519)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW 264.7 cells stimulated with LPS (HY-D1056)
Concentration:

100 μM
Incubation Time:

Pretreated with 1 h, then co-incubation for 4 h
Result:

Inhibited the IκBα phosphorylation levels.

Chemical Information

CAS No. 3061-91-4
Appearance Solid
Molecular Weight 280.32
Formula C₁₄H₂₀N₂O₄
Color Off-white to light yellow
SMILES

OC1=CC=C(C=C1)C[C@@H](C(O)=O)NC([C@H](C(C)C)N)=O
Synonyms

Val-Tyr
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Molecules

Investigating Natural Product Inhibitors of IKKα: Insights from Integrative In Silico and Experimental Validation. [Abstract]

2025 May 2;30(9):2025. PMID: 40363830

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (356.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 17.5 mg/mL (62.43 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5674 mL

17.8368 mL

35.6735 mL

5 mM

0.7135 mL

3.5674 mL

7.1347 mL

10 mM

0.3567 mL

1.7837 mL

3.5674 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.92 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yasir M, et al. Investigating Natural Product Inhibitors of IKKα: Insights from Integrative In Silico and Experimental Validation. Molecules. 2025 May 2;30(9):2025. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.5674 mL	17.8368 mL	35.6735 mL	89.1838 mL
	5 mM	0.7135 mL	3.5674 mL	7.1347 mL	17.8368 mL
	10 mM	0.3567 mL	1.7837 mL	3.5674 mL	8.9184 mL
	15 mM	0.2378 mL	1.1891 mL	2.3782 mL	5.9456 mL
	20 mM	0.1784 mL	0.8918 mL	1.7837 mL	4.4592 mL
	25 mM	0.1427 mL	0.7135 mL	1.4269 mL	3.5674 mL
	30 mM	0.1189 mL	0.5946 mL	1.1891 mL	2.9728 mL
	40 mM	0.0892 mL	0.4459 mL	0.8918 mL	2.2296 mL
	50 mM	0.0713 mL	0.3567 mL	0.7135 mL	1.7837 mL
	60 mM	0.0595 mL	0.2973 mL	0.5946 mL	1.4864 mL
DMSO	80 mM	0.0446 mL	0.2230 mL	0.4459 mL	1.1148 mL
DMSO	100 mM	0.0357 mL	0.1784 mL	0.3567 mL	0.8918 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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H-Val-Tyr-OH

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

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Publications Citing Use of MedChemExpress (MCE) H-Val-Tyr-OH

All IKK Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

H-Val-Tyr-OH Related Classifications

HY-W009338 Related Classifications