Steviol
Based on 1 Customer Validation
Steviol is the main metabolite of steviol glycosides and an inhibitor of AQP2/CFTR. Steviol slows down the growth of renal cysts by inhibiting the activity of CFTR, reducing the expression of AQP2, and promoting the degradation of AQP2 and CFTR. Steviol can be used in the research of polycystic kidney disease.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 471-80-7
- Formula: C20H30O3
- Molecular Weight:318.45
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Membrane Transporter/Ion Channel Compound Library
HY-L011
-
Natural Product Library
HY-L021
-
Macrocyclic Compound Library
HY-L041
-
Lipid Compound Library
HY-L043
-
Medicine Food Homology Compound Library
HY-L055
-
Terpenoids Library
HY-L056
-
Traditional Chinese Medicine Active Compound Library
HY-L065
-
Food-Sourced Compound Library
HY-L094
-
Plant-Sourced Natural Product Library
HY-L115
-
Membrane Protein-targeted Compound Library
HY-L149
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Ion Channel Compound Library
HY-L166
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product Library Plus
HY-L021P
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Bioactive Compound Library
HY-L001
-
Fatty Acids Compound Library
HY-L182
-
RO5 Drug-like Natural Product Library
HY-L200
-
High-Throughput Bioactive Compound Library
HY-L205
-
High-Throughput Natural Product Library
HY-L206
-
Chinese Baijiu Compound Library
HY-L241
-
Natural Product Diversity Scaffold Library
HY-L245
-
Mass Spectrometry Natural Product Library
HY-L262
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>10 μM
Compound: 1
|
Antiproliferative activity human against A549 cells measured after 48 hrs by CCK8 assay
Antiproliferative activity human against A549 cells measured after 48 hrs by CCK8 assay
|
[PMID: 35943432] |
| HCT-116 | IC50 |
>10 μM
Compound: 1
|
Antiproliferative activity against human HCT-116 cells measured after 48 hrs by CCK8 assay
Antiproliferative activity against human HCT-116 cells measured after 48 hrs by CCK8 assay
|
[PMID: 35943432] |
| HeLa | IC50 |
>10 μM
Compound: 1
|
Antiproliferative activity against human HeLa cells measured after 48 hrs by CCK8 assay
Antiproliferative activity against human HeLa cells measured after 48 hrs by CCK8 assay
|
[PMID: 35943432] |
| Huh-7 | IC50 |
>10 μM
Compound: 1
|
Antiproliferative activity against human Huh-7 cells measured after 48 hrs by CCK8 assay
Antiproliferative activity against human Huh-7 cells measured after 48 hrs by CCK8 assay
|
[PMID: 35943432] |
| MCF7 | IC50 |
185 μM
Compound: 1
|
Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by inverted-phase contrast microscopy
Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by inverted-phase contrast microscopy
|
[PMID: 35943432] |
| MCF7 | IC50 |
185 μM
Compound: 2
|
Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay
Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay
|
[PMID: 30059803] |
| MDA-MB-231 | IC50 |
>10 μM
Compound: 1
|
Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by CCK8 assay
Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by CCK8 assay
|
[PMID: 35943432] |
| Raji | IC50 |
340 μM
Compound: 6
|
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique
|
[PMID: 19131254] |
Steviol (100 μM; 2-24 h) can reduce AQP2 protein expression in MDCK cell monolayers[1].
Steviol (100 μM; 17-21 days) can significantly reduce cyst diameter in the cyst model constructed with Pkd1⁻/⁻ mouse renal cystic epithelial cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MDCK cell monolayers
-
Concentration:100 μM
-
Incubation Time:2, 6, 12 and 24 h
-
Result:Inhibited the levels of AQP2 at 6 h and 24 h treatment.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 471-80-7
-
Appearance Solid
-
Molecular Weight 318.45
-
Formula C20H30O3
-
Color White to off-white
-
SMILES
C[C@@]1(C(O)=O)CCC[C@@]2(C)[C@@]3([H])[C@@]4(CC([C@@](CC3)(O)C4)=C)CC[C@]12[H]
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (314.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (280 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Noitem R, et al. Steviol slows renal cyst growth by reducing AQP2 expression and promoting AQP2 degradation. Biomed Pharmacother. 2018 May;101:754-762. [Content Brief]
[2]. Melis MS, et al. Steviol effect, a glycoside of Stevia rebaudiana, on glucose clearances in rats. Braz J Biol. 2009 May;69(2):371-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1402 mL | 15.7011 mL | 31.4021 mL | 78.5053 mL |
| 5 mM | 0.6280 mL | 3.1402 mL | 6.2804 mL | 15.7011 mL | |
| 10 mM | 0.3140 mL | 1.5701 mL | 3.1402 mL | 7.8505 mL | |
| 15 mM | 0.2093 mL | 1.0467 mL | 2.0935 mL | 5.2337 mL | |
| 20 mM | 0.1570 mL | 0.7851 mL | 1.5701 mL | 3.9253 mL | |
| 25 mM | 0.1256 mL | 0.6280 mL | 1.2561 mL | 3.1402 mL | |
| 30 mM | 0.1047 mL | 0.5234 mL | 1.0467 mL | 2.6168 mL | |
| 40 mM | 0.0785 mL | 0.3925 mL | 0.7851 mL | 1.9626 mL | |
| 50 mM | 0.0628 mL | 0.3140 mL | 0.6280 mL | 1.5701 mL | |
| 60 mM | 0.0523 mL | 0.2617 mL | 0.5234 mL | 1.3084 mL | |
| 80 mM | 0.0393 mL | 0.1963 mL | 0.3925 mL | 0.9813 mL | |
| 100 mM | 0.0314 mL | 0.1570 mL | 0.3140 mL | 0.7851 mL |
Steviol Related Classifications
HY-N2057 Related Classifications
Powered by Bioz