Cat. No.: HY-N0669

Stevioside

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Stevioside is an orally active sweetener that can be isolated from Stevia rebaudiana, with antihypertensive, antihyperglycemic, antioxidant, anti-inflammatory and anti-tumor activities.

For research use only. We do not sell to patients.

Purity: 99.32%
CAS No.: 57817-89-7
Formula: C₃₈H₆₀O₁₈
Molecular Weight:804.87

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Stevioside Chemical Structure

CAS No.: 57817-89-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Stevioside is an orally active sweetener that can be isolated from Stevia rebaudiana, with antihypertensive, antihyperglycemic, antioxidant, anti-inflammatory and anti-tumor activities^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
Raji	IC₅₀	360 μM Compound: 1	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique	[PMID: 19131254]

In Vitro

Stevioside (10 μM, 24-48 h) can enhance cytotoxicity of 5-Fluorouracil (5-Fu, HY-90006) to MDA-MB-231 and SKBR3cells^[1].
Stevioside (10 μM, 48 h) combinated with 5-Fu (HY-90006) could increase the ratio of Bax to Bcl-2 in MDA-MB-231 cells, thereby triggering apoptosis^[1].
Stevioside (10-1000 μM, 6 h) induces TNF-α and IL-1β release in THP-1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231
Concentration:

10 μM
Incubation Time:

24 h
Result:

Increased the rate of apoptosis in cells with combination of 5-Fu (HY-90006).
Increased ROS level significantly in MDA-MB-231.
Combinated with 5-Fu (HY-90006) could trigger early and late apoptotic events in cancer cells.

Western Blot Analysis^[2]

Cell Line:

THP-1 cells
Concentration:

1 mM
Incubation Time:

45 min
Result:

Inhibited the expression level of NF-κB and IKKβ.

In Vivo

Stevioside (20 mg/kg, p.o., daily, 10 days) prevents significant increase in glycaemia in NMRI Haan mice^[3].
Stevioside (50-200 mg/kg, i.v., one time) reduces blood pressure in a dose-dependent manner in spontaneously hypertensive rats^[4].
Stevioside (6.25-25 mg/kg, p.o., daily, 15 days) increases the proliferative response of in LPS stimulated B-lymphocytes in Balb/c mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Balb/c mice^[5]
Dosage:

6.25-25 mg/kg
Administration:

p.o., daily, 7 days
Result:

Didn’t show any change in the general behaviour of the test animals.
Increased antibody synthesis of 15.38% and delayed type hypersensitivity (DTH) response of 18% at the dose of 12.5 mg/kg.
Possessed macrophage stimulatory activity and increased phagocytic index.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 57817-89-7
Appearance Solid
Molecular Weight 804.87
Formula C₃₈H₆₀O₁₈
Color White to off-white
SMILES

C[C@]([C@]1([H])CC2)(CCC[C@@]1(C)C(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=O)[C@@]4([H])[C@]2(CC5=C)C[C@@]5(O[C@@](O[C@H](CO)[C@@H](O)[C@@H]6O)([H])[C@@H]6O[C@]([C@@H]([C@@H](O)[C@@H]7O)O)([H])O[C@@H]7CO)CC4
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Plants
- Compositae
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

All Cell Proliferation/Viability AssayCell Imaging/StainingBio/Physico-chemical AssayRT-PCRWB

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Journal Impact Factor

J Orthop Translat

Stevioside attenuates osteoarthritis via regulating Nrf2/HO-1/NF-κB pathway. [Abstract]

2022 Nov 15:38:190-202. PMID: 36439628

Stevioside purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Nov 15:38:190-202. [Abstract]

Stevioside (STE; 10, 20, 40 µM) significantly decreases the expression of iNOS and COX-2 in a dose-dependent manner compared to stimulation with IL-1β.
Mediators Inflamm

Selenium Nanoparticles Decorated With Stevioside Potentially Attenuate Fructose Palmitate Induced Lipid Accumulation in HepG2 Cells. [Abstract]

2025 Feb 13:2025:7942947. PMID: 39981401

Stevioside purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2025 Feb 13:2025:7942947. [Abstract]

Stevioside (SV, 2.5-5 μM). CCK8 assay of cell viability of HFP-induced-HepG2 cells treated with different concentrations of SeNPs and SV-SeNPs for 48 h.

Stevioside purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2025 Feb 13:2025:7942947. [Abstract]

Stevioside (SV, 2.5-5 μM). The changes of lipid droplets in HFP-induced-HepG2 cells after SeNPs and SV-SeNPs treatment for 48 h were tested by oil red O staining.

Stevioside purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2025 Feb 13:2025:7942947. [Abstract]

Stevioside (SV, 2.5-5 μM). Determination of TG content in HFP-induced-HepG2 cells incubated with SeNPs and SV-SeNPs for 48 h. The data represent the mean ± SD of three independent experiments.

Stevioside purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2025 Feb 13:2025:7942947. [Abstract]

Stevioside (SV, 2.5-5 μM). SV-SeNPs relieve cell steatosis by modulation of lipid metabolic gene expression in HFP-treated HepG2 cells. HFP-induced-HepG2 cells were incubated with SeNPs and SV-SeNPs for 48 h and followed by q-PCR analysis of the mRNA expression of ACC1, FASN, SCD1, SREBP1.

Stevioside purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2025 Feb 13:2025:7942947. [Abstract]

Stevioside (SV, 2.5-5 μM). Effects of SV-SeNPs on PI3K/AKT/Nrf2 signaling pathway and PPARα in HFP-treated HepG2 cells. (A) HFP-induced-HepG2 cells were incubated with SeNPs and SV-SeNPs for 48 h and followed by western blot analysis of expression of P-PI3K, P-AKT, Nrf2, and PPARα.
Microbiol Spectr

Potential quorum-sensing inhibitor of Hafnia alvei H4-theaflavin-3,3´-digallate analyzed by virtual screening and molecular simulation. [Abstract]

2023 Sep 21;11(5):e0267123. PMID: 37732782
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 175 mg/mL (217.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2424 mL

6.2122 mL

12.4244 mL

5 mM

0.2485 mL

1.2424 mL

2.4849 mL

10 mM

0.1242 mL

0.6212 mL

1.2424 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Khare N, et al. Stevioside mediated chemosensitization studies and cytotoxicity assay on breast cancer cell lines MDA-MB-231 and SKBR3. Saudi J Biol Sci. 2019 Nov;26(7):1596-1601. [Content Brief]

[2]. Boonkaewwan C, Toskulkao C, Vongsakul M. Anti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. J Agric Food Chem. 2006 Feb 8;54(3):785-9. [Content Brief]

[3]. Ilić V, Vukmirović S, Stilinović N, Čapo I, Arsenović M, Milijašević B. Insight into anti-diabetic effect of low dose of stevioside. Biomed Pharmacother. 2017 Jun;90:216-221. [Content Brief]

[4]. Chan P, et al. The effect of stevioside on blood pressure and plasma catecholamines in spontaneously hypertensive rats. Life Sci. [Content Brief]

[5]. Sehar I, Kaul A, Bani S, Pal HC, Saxena AK. Immune up regulatory response of a non-caloric natural sweetener, stevioside. Chem Biol Interact. 2008 May 28;173(2):115-21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2424 mL	6.2122 mL	12.4244 mL	31.0609 mL
	5 mM	0.2485 mL	1.2424 mL	2.4849 mL	6.2122 mL
	10 mM	0.1242 mL	0.6212 mL	1.2424 mL	3.1061 mL
	15 mM	0.0828 mL	0.4141 mL	0.8283 mL	2.0707 mL
	20 mM	0.0621 mL	0.3106 mL	0.6212 mL	1.5530 mL
	25 mM	0.0497 mL	0.2485 mL	0.4970 mL	1.2424 mL
	30 mM	0.0414 mL	0.2071 mL	0.4141 mL	1.0354 mL
	40 mM	0.0311 mL	0.1553 mL	0.3106 mL	0.7765 mL
	50 mM	0.0248 mL	0.1242 mL	0.2485 mL	0.6212 mL
	60 mM	0.0207 mL	0.1035 mL	0.2071 mL	0.5177 mL
	80 mM	0.0155 mL	0.0777 mL	0.1553 mL	0.3883 mL
	100 mM	0.0124 mL	0.0621 mL	0.1242 mL	0.3106 mL

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Stevioside

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Publications Citing Use of MedChemExpress (MCE) Stevioside

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Stevioside Related Classifications

HY-N0669 Related Classifications