Boserolimab
Based on 1 Customer Validation
Boserolimab (MK-5890) is a humanized agonist monoclonal antibody that binds to CD27 to provide a costimulatory signal that enhances T-cell–mediated responses.
For research use only. We do not sell to patients.
- Purity : 99.00%
- CAS No.: 2444297-08-7
- Molecular Weight:144.91 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
CD27
In Vitro
Boserolimab (0-20 µg/mL, 3 days) inhibits the binding of CD27 to CD70 with an IC50 of 0.24 nM, activates NF-κB signaling pathway, thereby promoting the activation of T cell, improving the cytokine (IFNγ) secretion[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse MC38 xenograft models[1]
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Dosage:5 mg/kg
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Administration:ip, 3 times weekly for 3-7 doses
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Result:Inhibited tumor growth.
Reduced the number of CD4+, CD8+ T cells and Treg cells in peripheral blood.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Human IgG1 kappa
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1X106 Raji cells with Boserolimab (HY-P990018, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Loaded CD27/TNFRSF7 Protein (HEK293,His-Fc, HY-P72742) on NTA biosensor, can bind Boserolimab with an affinity constant of 8.578E-10 M as determined in BLI assay.
Chemical Information
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CAS No. 2444297-08-7
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Appearance Liquid
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Molecular Weight 144.91 kDa
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Color Colorless to light yellow
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SMILES
[Boserolimab]
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Synonyms
MK-5890
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (231 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)