CU-T12-9
Based on 13 publication(s) in Google Scholar
CU-T12-9 is a specific TLR1/2 agonist with EC50 of 52.9 nM in HEK-Blue hTLR2 SEAP assay. CU-T12-9 activates both the innate and the adaptive immune systems. CU-T12-9 selectively activates the TLR1/2 heterodimer, not TLR2/6. CU-T12-9 signals through NF-κB and invokes an elevation of the downstream effectors TNF-α, IL-10, and iNOS.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 1821387-73-8
- Formula: C17H13F3N4O2
- Molecular Weight:362.31
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) CU-T12-9
More- Nat Commun. 2024 Feb 6;15(1):1122. [Abstract]
- J Hazard Mater. 2023 Jun 15:452:131262. [Abstract]
- Cancer Lett. 2024 May 28:590:216839. [Abstract]
- Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
- Phytomedicine. 2026 Apr:153:157971. [Abstract]
- Front Immunol. 2026 Feb 9:16:1762269. [Abstract]
- Int Immunopharmacol. 2026 May 1:176:116462. [Abstract]
- Int Immunopharmacol. 2024 Aug 1:140:112702. [Abstract]
- Sci Rep. 2024 Nov 29;14(1):29740. [Abstract]
- Mol Immunol. 2025 Mar 28:182:1-10. [Abstract]
- Immun Inflamm Dis. 2023 Oct;11(10):e970. [Abstract]
- Mol Biotechnol. 2025 May 1. [Abstract]
- Res Sq. 2025 Sep 1.
Customer Validation & Images
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In Vivo Efficacy Study
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Histological Imaging/Staining
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WB
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RT-PCR
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Apoptosis Analysis
Biological Activity
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TLR2 52.9 nM (EC50, in HEK-Blue cells) |
TLR1 52.9 nM (EC50, in HEK-Blue cells) |
CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. By binding to both TLR1 and TLR2, CU-T12-9 facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream signaling[1].
CU-T12-9 activates the TLR1/2 pathway by inducing NF-κB activation to trigger downstream signaling, such as secreted embryonic alkaline phosphatase (SEAP), NO, and TNF-α[1].
CU-T12-9 (0.39-100 μM; 24 hours) does not produce toxicity up to 100 μM in HEK-Blue hTLR2 and Raw 264.7 cells[1].
CU-T12-9 up-regulates the mRNA levels of TLR1, TLR2, TNF, IL-10, and iNOS. CU-T12-9 (0.1-10 μM) activates TLR1 mRNA and iNOS mRNA after Raw 264.7 cells are treated for 24 hours. CU-T12-9 (0.1-10 μM) activates TLR2 and IL-10 mRNA after Raw 264.7 cells are treated for 2 hours. CU-T12-9 (0.1-10 μM) activates TNF mRNA after Raw 264.7 cells are treated for 8 hours[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK-Blue hTLR2 and Raw 264.7 macrophage cells
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Concentration:0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 μM
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Incubation Time:24 hours
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Result:No toxicity was seen up to 100 μM.
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Cell Line:Raw 264.7 cells
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Concentration:0.1, 1, 10 μM
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Incubation Time:24 hours for TLR1 and iNOS mRNA assay
2 hours for TLR2 and IL-10 mRNA assay
8 hours for TNF mRNA assay -
Result:Triggered TLR1 mRNA and iNOS mRNA at 24 hours dose-dependently.
Dose-dependent activation of TLR2 mRNA and IL-10 mRNA at 2 hours.
Showed dose-dependent activation of TNF mRNA at 8 hours.
Chemical Information
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CAS No. 1821387-73-8
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Appearance Solid
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Molecular Weight 362.31
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Formula C17H13F3N4O2
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Color Light yellow to yellow
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SMILES
CNC1=CC=C(C=C1N2C=C(N=C2)C3=CC=C(C=C3)C(F)(F)F)[N+]([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (13)
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Journal Impact Factor
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Most Recent
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Nat Commun
AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation. [Abstract]2024 Feb 6;15(1):1122. PMID: 38321056 -
J Hazard Mater
Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1. [Abstract]2023 Jun 15:452:131262. PMID: 36989784 -
Cancer Lett
JAML promotes the antitumor role of tumor-resident CD8+ T cells by facilitating their innate-like function in human lung cancer. [Abstract]2024 May 28:590:216839. PMID: 38570084 -
Cell Commun Signal
Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction. [Abstract]2025 Jan 31;23(1):58. PMID: 39891142
CU-T12-9 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
Testis sizes comparisons among the control group, IR group, Amifostine group, NF-κB activator 2 group, and CU-T12-9 (5 mg/kg, i.p.) group.
CU-T12-9 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
HE staining of testis among the control group, IR group, Amifostine group, NF-κB activator 2 group, and CU-T12-9 (5 mg/kg, i.p.) group.
CU-T12-9 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
Protein level alterations of Bclaf1, P-Rela, Rela, Sycp3, and β-actin were analyzed among the control group, IR group, Amifostine group, NF-κB activator 2 group, and CU-T12-9 (5 mg/kg, i.p.) group.
CU-T12-9 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jan 31;23(1):58. [Abstract]
RT-qPCR analysis of Spliceosome-related gene expression levels across the control group, IR group, Amifostine group, NF-κB activator 2 group, and CU-T12-9 (5 mg/kg, i.p.) group.
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Phytomedicine
Xanthoxylin alleviates dextran sulfate sodium (DSS)-induced colitis by targeting macrophage infiltration via the tumor necrosis factor (TNF) /nuclear factor-kappa B (NF-κB) signaling pathway. [Abstract]2026 Apr:153:157971. PMID: 41720014 -
Front Immunol
Activation of NF-κB signaling in tissue-resident memory T cells promotes recurrent psoriasis in mice. [Abstract]2026 Feb 9:16:1762269. PMID: 41737494 -
Int Immunopharmacol
2026 May 1:176:116462. PMID: 41797086 -
Int Immunopharmacol
Electroacupuncture and methotrexate cooperate to ameliorate psoriasiform skin inflammation by regulating the immune balance of Th17/Treg. [Abstract]2024 Aug 1:140:112702. PMID: 39094355 -
Sci Rep
Triptolide induces apoptosis of glioma cells by inhibiting NF-κB activation during oxidative stress. [Abstract]2024 Nov 29;14(1):29740. PMID: 39614071
CU-T12-9 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 29;14(1):29740. [Abstract]
The apoptosis level was evaluated by TUNEL assay after CU-T12-9 (20 μM) treatment.
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Mol Immunol
Photobiomodulation regulates inflammation and autophagy in spinal cord injury through NLRP3/Caspase-1/IL-1β pathway by targeting TLR2. [Abstract]2025 Mar 28:182:1-10. PMID: 40157277 -
Immun Inflamm Dis
TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation. [Abstract]2023 Oct;11(10):e970. PMID: 37904691 -
Mol Biotechnol
Curcumin Inhibits Lipopolysaccharide-Induced Inflammation Through the HMGB1/NF-κB Signaling Pathway to Promote the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. [Abstract]2025 May 1. PMID: 40310592 -
Solvent & Solubility
DMSO : 250 mg/mL (690.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7601 mL | 13.8003 mL | 27.6007 mL | 69.0017 mL |
| 5 mM | 0.5520 mL | 2.7601 mL | 5.5201 mL | 13.8003 mL | |
| 10 mM | 0.2760 mL | 1.3800 mL | 2.7601 mL | 6.9002 mL | |
| 15 mM | 0.1840 mL | 0.9200 mL | 1.8400 mL | 4.6001 mL | |
| 20 mM | 0.1380 mL | 0.6900 mL | 1.3800 mL | 3.4501 mL | |
| 25 mM | 0.1104 mL | 0.5520 mL | 1.1040 mL | 2.7601 mL | |
| 30 mM | 0.0920 mL | 0.4600 mL | 0.9200 mL | 2.3001 mL | |
| 40 mM | 0.0690 mL | 0.3450 mL | 0.6900 mL | 1.7250 mL | |
| 50 mM | 0.0552 mL | 0.2760 mL | 0.5520 mL | 1.3800 mL | |
| 60 mM | 0.0460 mL | 0.2300 mL | 0.4600 mL | 1.1500 mL | |
| 80 mM | 0.0345 mL | 0.1725 mL | 0.3450 mL | 0.8625 mL | |
| 100 mM | 0.0276 mL | 0.1380 mL | 0.2760 mL | 0.6900 mL |
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